Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2589 and Dsui_2590 are separated by 2 nucleotides Dsui_2590 and Dsui_2591 are separated by 612 nucleotides
Dsui_2589: Dsui_2589 - type II secretory pathway, component PulD, at 2,742,273 to 2,743,994
_2589
Dsui_2590: Dsui_2590 - Pilin accessory protein (PilO), at 2,743,997 to 2,745,409
_2590
Dsui_2591: Dsui_2591 - type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 2,746,022 to 2,747,608
_2591
Position (kb)
2743
2744
2745
2746 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2743.312 kb on - strand at 2743.472 kb on + strand, within Dsui_2589 at 2743.552 kb on + strand, within Dsui_2589 at 2743.560 kb on - strand, within Dsui_2589 at 2743.641 kb on - strand, within Dsui_2589 at 2743.652 kb on - strand, within Dsui_2589 at 2743.654 kb on + strand, within Dsui_2589 at 2743.662 kb on - strand, within Dsui_2589 at 2743.666 kb on + strand, within Dsui_2589 at 2743.676 kb on + strand, within Dsui_2589 at 2743.678 kb on + strand, within Dsui_2589 at 2743.678 kb on + strand, within Dsui_2589 at 2743.679 kb on - strand, within Dsui_2589 at 2743.768 kb on + strand, within Dsui_2589 at 2743.768 kb on + strand, within Dsui_2589 at 2744.026 kb on - strand at 2744.408 kb on + strand, within Dsui_2590 at 2744.497 kb on - strand, within Dsui_2590 at 2744.571 kb on - strand, within Dsui_2590 at 2744.573 kb on + strand, within Dsui_2590 at 2744.676 kb on - strand, within Dsui_2590 at 2744.732 kb on + strand, within Dsui_2590 at 2744.735 kb on + strand at 2744.752 kb on + strand, within Dsui_2590 at 2744.762 kb on - strand, within Dsui_2590 at 2744.855 kb on + strand, within Dsui_2590 at 2744.932 kb on + strand at 2745.216 kb on - strand, within Dsui_2590 at 2745.221 kb on - strand, within Dsui_2590 at 2745.274 kb on + strand at 2745.394 kb on + strand at 2745.572 kb on - strand at 2745.601 kb on - strand at 2745.787 kb on + strand at 2745.846 kb on + strand at 2745.872 kb on + strand at 2745.914 kb on + strand at 2746.008 kb on + strand at 2746.088 kb on + strand at 2746.142 kb on - strand at 2746.148 kb on + strand at 2746.217 kb on + strand, within Dsui_2591
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,743,312 - -0.8 2,743,472 + Dsui_2589 0.70 +1.5 2,743,552 + Dsui_2589 0.74 +2.1 2,743,560 - Dsui_2589 0.75 +1.6 2,743,641 - Dsui_2589 0.79 +0.6 2,743,652 - Dsui_2589 0.80 +0.6 2,743,654 + Dsui_2589 0.80 +0.1 2,743,662 - Dsui_2589 0.81 -0.8 2,743,666 + Dsui_2589 0.81 -1.3 2,743,676 + Dsui_2589 0.81 +0.6 2,743,678 + Dsui_2589 0.82 +0.1 2,743,678 + Dsui_2589 0.82 -0.7 2,743,679 - Dsui_2589 0.82 +0.1 2,743,768 + Dsui_2589 0.87 -0.5 2,743,768 + Dsui_2589 0.87 -0.3 2,744,026 - +0.7 2,744,408 + Dsui_2590 0.29 -1.5 2,744,497 - Dsui_2590 0.35 +0.0 2,744,571 - Dsui_2590 0.41 +0.5 2,744,573 + Dsui_2590 0.41 -1.5 2,744,676 - Dsui_2590 0.48 -0.0 2,744,732 + Dsui_2590 0.52 -0.3 2,744,735 + +1.6 2,744,752 + Dsui_2590 0.53 +1.5 2,744,762 - Dsui_2590 0.54 +1.3 2,744,855 + Dsui_2590 0.61 -0.4 2,744,932 + +1.5 2,745,216 - Dsui_2590 0.86 -1.7 2,745,221 - Dsui_2590 0.87 -0.5 2,745,274 + +1.5 2,745,394 + +0.6 2,745,572 - -0.0 2,745,601 - -1.6 2,745,787 + +0.9 2,745,846 + -2.7 2,745,872 + -1.0 2,745,914 + +3.0 2,746,008 + +0.2 2,746,088 + -0.2 2,746,142 - +2.0 2,746,148 + +0.4 2,746,217 + Dsui_2591 0.12 +1.0
Or see this region's nucleotide sequence