Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2457 and Dsui_2458 are separated by 11 nucleotides Dsui_2458 and Dsui_2459 are separated by 16 nucleotides Dsui_2459 and Dsui_2460 overlap by 8 nucleotides Dsui_2460 and Dsui_2461 overlap by 4 nucleotides
Dsui_2457: Dsui_2457 - GTP-binding protein LepA, at 2,615,057 to 2,616,850
_2457
Dsui_2458: Dsui_2458 - signal peptidase I, at 2,616,862 to 2,617,650
_2458
Dsui_2459: Dsui_2459 - hypothetical protein, at 2,617,667 to 2,618,038
_2459
Dsui_2460: Dsui_2460 - ribonuclease III, at 2,618,031 to 2,618,723
_2460
Dsui_2461: Dsui_2461 - GTP-binding protein Era, at 2,618,720 to 2,619,613
_2461
Position (kb)
2617
2618
2619 Strain fitness (log2 ratio)
-2
-1
0
1 at 2617.649 kb on + strand at 2617.670 kb on - strand at 2617.873 kb on + strand, within Dsui_2459 at 2617.887 kb on + strand, within Dsui_2459 at 2618.023 kb on + strand at 2618.023 kb on + strand at 2618.023 kb on + strand at 2618.031 kb on - strand at 2618.031 kb on - strand at 2618.031 kb on - strand at 2618.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,617,649 + -2.6 2,617,670 - +0.9 2,617,873 + Dsui_2459 0.55 +0.0 2,617,887 + Dsui_2459 0.59 -0.7 2,618,023 + -0.4 2,618,023 + -0.5 2,618,023 + +0.1 2,618,031 - +0.9 2,618,031 - +0.1 2,618,031 - -0.1 2,618,031 - +0.4
Or see this region's nucleotide sequence