Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2295 and Dsui_2296 are separated by 304 nucleotides Dsui_2296 and Dsui_2297 are separated by 158 nucleotides
Dsui_2295: Dsui_2295 - transcriptional regulator, at 2,449,202 to 2,449,843
_2295
Dsui_2296: Dsui_2296 - chemotaxis protein, at 2,450,148 to 2,450,747
_2296
Dsui_2297: Dsui_2297 - acetoacetyl-CoA synthase, at 2,450,906 to 2,452,876
_2297
Position (kb)
2450
2451 Strain fitness (log2 ratio)
-2
-1
0
1 at 2449.162 kb on + strand at 2449.263 kb on + strand at 2449.506 kb on + strand, within Dsui_2295 at 2449.890 kb on - strand at 2449.898 kb on + strand at 2450.150 kb on - strand at 2450.187 kb on + strand at 2450.214 kb on + strand, within Dsui_2296 at 2450.420 kb on + strand, within Dsui_2296 at 2450.487 kb on + strand, within Dsui_2296 at 2450.563 kb on + strand, within Dsui_2296 at 2450.674 kb on - strand at 2450.741 kb on + strand at 2450.839 kb on - strand at 2450.938 kb on + strand at 2450.992 kb on + strand at 2450.992 kb on - strand at 2451.061 kb on - strand at 2451.139 kb on + strand, within Dsui_2297 at 2451.139 kb on + strand, within Dsui_2297 at 2451.407 kb on + strand at 2451.437 kb on + strand, within Dsui_2297 at 2451.528 kb on + strand, within Dsui_2297 at 2451.563 kb on + strand, within Dsui_2297 at 2451.574 kb on + strand, within Dsui_2297
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,449,162 + +0.6 2,449,263 + +1.6 2,449,506 + Dsui_2295 0.47 -0.5 2,449,890 - +0.6 2,449,898 + +0.3 2,450,150 - -0.5 2,450,187 + -0.5 2,450,214 + Dsui_2296 0.11 -0.7 2,450,420 + Dsui_2296 0.45 -0.4 2,450,487 + Dsui_2296 0.56 -1.0 2,450,563 + Dsui_2296 0.69 -0.2 2,450,674 - +0.4 2,450,741 + -0.1 2,450,839 - -2.4 2,450,938 + -0.3 2,450,992 + +0.3 2,450,992 - -0.9 2,451,061 - -1.9 2,451,139 + Dsui_2297 0.12 -0.3 2,451,139 + Dsui_2297 0.12 -0.6 2,451,407 + +0.6 2,451,437 + Dsui_2297 0.27 -2.0 2,451,528 + Dsui_2297 0.32 +0.1 2,451,563 + Dsui_2297 0.33 +0.7 2,451,574 + Dsui_2297 0.34 +0.6
Or see this region's nucleotide sequence