Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2140 and Dsui_2141 overlap by 1 nucleotides Dsui_2141 and Dsui_2142 overlap by 26 nucleotides
Dsui_2140: Dsui_2140 - hypothetical protein, at 2,268,527 to 2,270,617
_2140
Dsui_2141: Dsui_2141 - flagellar motor protein, at 2,270,617 to 2,271,261
_2141
Dsui_2142: Dsui_2142 - Protein of unknown function (DUF2894), at 2,271,236 to 2,272,111
_2142
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2269.670 kb on - strand, within Dsui_2140 at 2269.892 kb on - strand at 2269.986 kb on - strand at 2270.009 kb on + strand, within Dsui_2140 at 2270.014 kb on + strand, within Dsui_2140 at 2270.132 kb on + strand, within Dsui_2140 at 2270.214 kb on + strand, within Dsui_2140 at 2270.224 kb on + strand, within Dsui_2140 at 2270.381 kb on + strand, within Dsui_2140 at 2270.381 kb on + strand, within Dsui_2140 at 2270.393 kb on + strand, within Dsui_2140 at 2270.580 kb on + strand at 2270.634 kb on - strand at 2270.650 kb on + strand at 2270.699 kb on + strand, within Dsui_2141 at 2270.699 kb on + strand, within Dsui_2141 at 2270.753 kb on - strand, within Dsui_2141 at 2270.753 kb on - strand at 2270.815 kb on + strand, within Dsui_2141 at 2270.889 kb on + strand, within Dsui_2141 at 2271.020 kb on - strand, within Dsui_2141 at 2271.154 kb on - strand at 2271.264 kb on - strand at 2271.866 kb on + strand, within Dsui_2142 at 2271.877 kb on + strand, within Dsui_2142 at 2271.885 kb on - strand at 2272.006 kb on + strand at 2272.019 kb on - strand at 2272.019 kb on - strand at 2272.038 kb on - strand at 2272.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,269,670 - Dsui_2140 0.55 +0.6 2,269,892 - -0.8 2,269,986 - +0.0 2,270,009 + Dsui_2140 0.71 -0.5 2,270,014 + Dsui_2140 0.71 -0.5 2,270,132 + Dsui_2140 0.77 -2.3 2,270,214 + Dsui_2140 0.81 -0.0 2,270,224 + Dsui_2140 0.81 +0.4 2,270,381 + Dsui_2140 0.89 +0.1 2,270,381 + Dsui_2140 0.89 +0.2 2,270,393 + Dsui_2140 0.89 +0.0 2,270,580 + +0.5 2,270,634 - +2.0 2,270,650 + +0.3 2,270,699 + Dsui_2141 0.13 -0.8 2,270,699 + Dsui_2141 0.13 -0.9 2,270,753 - Dsui_2141 0.21 -0.5 2,270,753 - -1.3 2,270,815 + Dsui_2141 0.31 -1.7 2,270,889 + Dsui_2141 0.42 -0.6 2,271,020 - Dsui_2141 0.62 -0.2 2,271,154 - -0.4 2,271,264 - -0.2 2,271,866 + Dsui_2142 0.72 +0.3 2,271,877 + Dsui_2142 0.73 +0.9 2,271,885 - -0.5 2,272,006 + +1.0 2,272,019 - +1.0 2,272,019 - +0.1 2,272,038 - -2.1 2,272,184 - -0.6
Or see this region's nucleotide sequence