Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0246 and Dsui_0248 are separated by 844 nucleotides Dsui_0248 and Dsui_0249 overlap by 4 nucleotides Dsui_0249 and Dsui_0250 are separated by 39 nucleotides
Dsui_0246: Dsui_0246 - mraZ protein, at 246,217 to 246,666
_0246
Dsui_0248: Dsui_0248 - Protein of unknown function (DUF3025), at 247,511 to 248,326
_0248
Dsui_0249: Dsui_0249 - hypothetical protein, at 248,323 to 248,832
_0249
Dsui_0250: Dsui_0250 - dihydroorotase, homodimeric type, at 248,872 to 249,906
_0250
Position (kb) 247
248
249 Strain fitness (log2 ratio) -1
0
1
2 at 246.527 kb on - strand, within Dsui_0246 at 246.532 kb on - strand, within Dsui_0246 at 246.538 kb on - strand, within Dsui_0246 at 246.625 kb on - strand at 246.690 kb on - strand at 246.728 kb on - strand at 246.753 kb on - strand at 246.984 kb on + strand at 247.026 kb on - strand at 247.072 kb on - strand at 247.518 kb on + strand at 247.518 kb on + strand at 247.521 kb on - strand at 247.771 kb on - strand, within Dsui_0248 at 247.876 kb on - strand, within Dsui_0248 at 247.914 kb on - strand at 247.932 kb on - strand, within Dsui_0248 at 248.030 kb on + strand, within Dsui_0248 at 248.035 kb on + strand at 248.051 kb on + strand at 248.189 kb on + strand, within Dsui_0248 at 248.427 kb on + strand, within Dsui_0249 at 248.434 kb on + strand, within Dsui_0249 at 248.571 kb on + strand at 248.579 kb on - strand, within Dsui_0249
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 246,527 - Dsui_0246 0.69 -1.5 246,532 - Dsui_0246 0.70 +0.1 246,538 - Dsui_0246 0.71 -0.4 246,625 - -0.1 246,690 - -0.2 246,728 - -0.4 246,753 - -0.3 246,984 + +0.5 247,026 - -0.7 247,072 - +0.4 247,518 + +0.3 247,518 + -0.9 247,521 - +0.3 247,771 - Dsui_0248 0.32 -0.1 247,876 - Dsui_0248 0.45 -0.1 247,914 - +1.8 247,932 - Dsui_0248 0.52 -0.8 248,030 + Dsui_0248 0.64 -1.3 248,035 + +0.0 248,051 + +0.1 248,189 + Dsui_0248 0.83 -0.1 248,427 + Dsui_0249 0.20 -0.4 248,434 + Dsui_0249 0.22 -1.5 248,571 + -1.7 248,579 - Dsui_0249 0.50 -0.5
Or see this region's nucleotide sequence