Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0025 and Dsui_0026 are separated by 129 nucleotides Dsui_0026 and Dsui_0027 are separated by 214 nucleotides Dsui_0027 and Dsui_0028 overlap by 4 nucleotides
Dsui_0025: Dsui_0025 - hypothetical protein, at 22,628 to 23,710
_0025
Dsui_0026: Dsui_0026 - CBS domain-containing protein, at 23,840 to 25,126
_0026
Dsui_0027: Dsui_0027 - putative effector of murein hydrolase LrgA, at 25,341 to 25,703
_0027
Dsui_0028: Dsui_0028 - putative effector of murein hydrolase, at 25,700 to 26,455
_0028
Position (kb) 23
24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2 at 22.882 kb on - strand at 22.992 kb on + strand, within Dsui_0025 at 23.197 kb on + strand at 23.322 kb on + strand, within Dsui_0025 at 23.381 kb on + strand, within Dsui_0025 at 23.577 kb on - strand, within Dsui_0025 at 23.589 kb on - strand at 23.753 kb on - strand at 23.787 kb on - strand at 23.807 kb on - strand at 23.825 kb on - strand at 23.858 kb on + strand at 23.892 kb on - strand at 23.965 kb on + strand at 24.096 kb on + strand, within Dsui_0026 at 24.136 kb on + strand, within Dsui_0026 at 24.200 kb on - strand, within Dsui_0026 at 24.204 kb on - strand at 24.215 kb on - strand at 24.230 kb on - strand, within Dsui_0026 at 24.264 kb on - strand, within Dsui_0026 at 24.344 kb on + strand, within Dsui_0026 at 24.364 kb on - strand, within Dsui_0026 at 24.364 kb on - strand, within Dsui_0026 at 24.378 kb on + strand, within Dsui_0026 at 24.386 kb on - strand, within Dsui_0026 at 24.455 kb on - strand at 24.556 kb on + strand at 24.556 kb on - strand at 24.566 kb on - strand, within Dsui_0026 at 24.615 kb on + strand, within Dsui_0026 at 24.637 kb on + strand, within Dsui_0026 at 24.686 kb on + strand at 24.706 kb on + strand, within Dsui_0026 at 24.741 kb on + strand at 24.749 kb on - strand, within Dsui_0026 at 24.817 kb on + strand at 24.823 kb on + strand, within Dsui_0026 at 24.831 kb on - strand, within Dsui_0026 at 24.853 kb on + strand at 24.861 kb on - strand, within Dsui_0026 at 24.918 kb on - strand, within Dsui_0026 at 24.976 kb on - strand, within Dsui_0026 at 25.099 kb on - strand at 25.190 kb on - strand at 25.233 kb on - strand at 25.287 kb on - strand at 25.355 kb on + strand at 25.396 kb on + strand at 25.480 kb on + strand at 25.498 kb on - strand at 25.596 kb on + strand, within Dsui_0027 at 25.660 kb on - strand, within Dsui_0027 at 25.667 kb on - strand at 25.667 kb on - strand at 25.739 kb on + strand at 25.741 kb on - strand at 25.777 kb on - strand, within Dsui_0028 at 25.785 kb on + strand, within Dsui_0028 at 25.793 kb on - strand at 25.828 kb on - strand at 25.979 kb on + strand, within Dsui_0028 at 26.007 kb on - strand, within Dsui_0028 at 26.008 kb on + strand, within Dsui_0028 at 26.008 kb on + strand, within Dsui_0028 at 26.016 kb on - strand, within Dsui_0028 at 26.028 kb on - strand, within Dsui_0028 at 26.028 kb on - strand, within Dsui_0028 at 26.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 22,882 - -0.9 22,992 + Dsui_0025 0.34 -0.2 23,197 + +0.1 23,322 + Dsui_0025 0.64 -0.3 23,381 + Dsui_0025 0.70 -0.2 23,577 - Dsui_0025 0.88 -0.7 23,589 - -1.0 23,753 - -0.9 23,787 - -0.1 23,807 - -0.6 23,825 - -0.9 23,858 + -0.2 23,892 - -0.1 23,965 + -0.2 24,096 + Dsui_0026 0.20 -0.2 24,136 + Dsui_0026 0.23 -1.0 24,200 - Dsui_0026 0.28 -0.2 24,204 - +0.1 24,215 - -0.6 24,230 - Dsui_0026 0.30 -0.3 24,264 - Dsui_0026 0.33 -0.4 24,344 + Dsui_0026 0.39 -0.9 24,364 - Dsui_0026 0.41 -0.1 24,364 - Dsui_0026 0.41 -1.8 24,378 + Dsui_0026 0.42 -0.5 24,386 - Dsui_0026 0.42 +0.1 24,455 - +1.8 24,556 + -1.1 24,556 - +0.6 24,566 - Dsui_0026 0.56 -0.8 24,615 + Dsui_0026 0.60 -0.0 24,637 + Dsui_0026 0.62 +0.0 24,686 + -0.1 24,706 + Dsui_0026 0.67 -0.5 24,741 + -0.5 24,749 - Dsui_0026 0.71 -0.4 24,817 + +0.0 24,823 + Dsui_0026 0.76 -1.3 24,831 - Dsui_0026 0.77 -0.4 24,853 + +0.9 24,861 - Dsui_0026 0.79 -0.6 24,918 - Dsui_0026 0.84 -0.4 24,976 - Dsui_0026 0.88 -0.2 25,099 - -0.5 25,190 - -0.3 25,233 - +0.5 25,287 - +0.7 25,355 + +0.1 25,396 + +0.5 25,480 + +1.1 25,498 - -1.1 25,596 + Dsui_0027 0.70 -0.3 25,660 - Dsui_0027 0.88 +0.7 25,667 - -0.5 25,667 - -0.5 25,739 + +0.1 25,741 - -0.3 25,777 - Dsui_0028 0.10 -0.0 25,785 + Dsui_0028 0.11 -0.3 25,793 - -0.5 25,828 - +0.5 25,979 + Dsui_0028 0.37 +0.0 26,007 - Dsui_0028 0.41 -0.2 26,008 + Dsui_0028 0.41 -0.0 26,008 + Dsui_0028 0.41 -0.3 26,016 - Dsui_0028 0.42 -0.1 26,028 - Dsui_0028 0.43 -0.3 26,028 - Dsui_0028 0.43 -0.9 26,044 + +0.3
Or see this region's nucleotide sequence