Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phhA and Sama_2223 are separated by 358 nucleotides Sama_2223 and Sama_2224 are separated by 2 nucleotides
Sama_2222: phhA - phenylalanine 4-monooxygenase (RefSeq), at 2,685,807 to 2,686,598
phhA
Sama_2223: Sama_2223 - UTP--glucose-1-phosphate uridylyltransferase (RefSeq), at 2,686,957 to 2,687,865
_2223
Sama_2224: Sama_2224 - UDP-glucose 4-epimerase (RefSeq), at 2,687,868 to 2,688,878
_2224
Position (kb)
2686
2687
2688 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2685.957 kb on - strand, within phhA at 2686.010 kb on + strand, within phhA at 2686.010 kb on + strand, within phhA at 2686.010 kb on + strand, within phhA at 2686.024 kb on + strand, within phhA at 2686.025 kb on - strand, within phhA at 2686.064 kb on - strand, within phhA at 2686.064 kb on - strand, within phhA at 2686.067 kb on + strand, within phhA at 2686.067 kb on + strand, within phhA at 2686.067 kb on + strand, within phhA at 2686.068 kb on - strand, within phhA at 2686.112 kb on + strand, within phhA at 2686.113 kb on - strand, within phhA at 2686.135 kb on + strand, within phhA at 2686.135 kb on + strand, within phhA at 2686.135 kb on + strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.177 kb on + strand, within phhA at 2686.177 kb on + strand, within phhA at 2686.178 kb on - strand, within phhA at 2686.178 kb on - strand, within phhA at 2686.178 kb on - strand, within phhA at 2686.264 kb on + strand, within phhA at 2686.265 kb on - strand, within phhA at 2686.265 kb on - strand, within phhA at 2686.290 kb on - strand, within phhA at 2686.465 kb on + strand, within phhA at 2686.465 kb on + strand, within phhA at 2686.466 kb on - strand, within phhA at 2686.466 kb on - strand, within phhA at 2686.466 kb on - strand, within phhA at 2686.510 kb on + strand, within phhA at 2686.511 kb on - strand, within phhA at 2686.511 kb on - strand, within phhA at 2686.554 kb on + strand at 2686.555 kb on - strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.592 kb on + strand at 2686.592 kb on + strand at 2686.592 kb on + strand at 2686.634 kb on + strand at 2686.635 kb on - strand at 2686.660 kb on - strand at 2686.675 kb on + strand at 2686.693 kb on + strand at 2686.763 kb on - strand at 2686.875 kb on + strand at 2686.875 kb on + strand at 2686.875 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.891 kb on - strand at 2686.946 kb on + strand at 2687.112 kb on + strand, within Sama_2223 at 2687.555 kb on + strand, within Sama_2223 at 2687.555 kb on + strand, within Sama_2223 at 2687.556 kb on - strand, within Sama_2223 at 2687.612 kb on + strand, within Sama_2223 at 2687.613 kb on - strand, within Sama_2223 at 2687.772 kb on + strand, within Sama_2223 at 2687.863 kb on + strand at 2687.863 kb on + strand at 2688.249 kb on + strand, within Sama_2224 at 2688.528 kb on + strand, within Sama_2224 at 2688.561 kb on + strand, within Sama_2224 at 2688.639 kb on + strand, within Sama_2224 at 2688.727 kb on + strand, within Sama_2224
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 2,685,957 - phhA Sama_2222 0.19 +0.5 2,686,010 + phhA Sama_2222 0.26 +0.2 2,686,010 + phhA Sama_2222 0.26 -1.4 2,686,010 + phhA Sama_2222 0.26 -1.1 2,686,024 + phhA Sama_2222 0.27 +1.5 2,686,025 - phhA Sama_2222 0.28 -0.6 2,686,064 - phhA Sama_2222 0.32 +3.9 2,686,064 - phhA Sama_2222 0.32 -1.7 2,686,067 + phhA Sama_2222 0.33 +0.5 2,686,067 + phhA Sama_2222 0.33 -2.5 2,686,067 + phhA Sama_2222 0.33 -1.1 2,686,068 - phhA Sama_2222 0.33 +0.2 2,686,112 + phhA Sama_2222 0.39 -0.1 2,686,113 - phhA Sama_2222 0.39 -1.1 2,686,135 + phhA Sama_2222 0.41 -0.3 2,686,135 + phhA Sama_2222 0.41 +1.1 2,686,135 + phhA Sama_2222 0.41 -0.4 2,686,136 - phhA Sama_2222 0.42 -1.7 2,686,136 - phhA Sama_2222 0.42 +1.6 2,686,136 - phhA Sama_2222 0.42 +3.7 2,686,136 - phhA Sama_2222 0.42 -0.5 2,686,177 + phhA Sama_2222 0.47 +2.7 2,686,177 + phhA Sama_2222 0.47 -0.5 2,686,178 - phhA Sama_2222 0.47 -1.1 2,686,178 - phhA Sama_2222 0.47 +1.4 2,686,178 - phhA Sama_2222 0.47 -0.5 2,686,264 + phhA Sama_2222 0.58 -2.7 2,686,265 - phhA Sama_2222 0.58 -1.2 2,686,265 - phhA Sama_2222 0.58 -0.6 2,686,290 - phhA Sama_2222 0.61 +1.2 2,686,465 + phhA Sama_2222 0.83 -1.7 2,686,465 + phhA Sama_2222 0.83 +2.3 2,686,466 - phhA Sama_2222 0.83 +2.6 2,686,466 - phhA Sama_2222 0.83 -1.4 2,686,466 - phhA Sama_2222 0.83 +2.5 2,686,510 + phhA Sama_2222 0.89 +0.6 2,686,511 - phhA Sama_2222 0.89 +1.4 2,686,511 - phhA Sama_2222 0.89 -1.5 2,686,554 + +1.4 2,686,555 - +0.8 2,686,585 + -2.1 2,686,585 + +3.7 2,686,585 + -1.7 2,686,585 + +1.4 2,686,585 + -1.5 2,686,585 + -1.0 2,686,586 - -1.6 2,686,586 - +0.6 2,686,586 - -1.3 2,686,586 - -1.3 2,686,586 - -3.4 2,686,586 - -1.3 2,686,586 - +2.0 2,686,586 - +0.7 2,686,586 - -0.5 2,686,586 - +0.3 2,686,592 + -0.8 2,686,592 + +0.3 2,686,592 + -0.9 2,686,634 + -1.8 2,686,635 - -2.2 2,686,660 - -1.3 2,686,675 + +0.3 2,686,693 + +0.0 2,686,763 - -3.1 2,686,875 + +2.0 2,686,875 + -3.4 2,686,875 + -1.5 2,686,890 + -2.8 2,686,890 + +1.8 2,686,890 + -1.4 2,686,890 + -1.0 2,686,890 + +1.8 2,686,890 + +0.1 2,686,890 + -3.2 2,686,890 + -2.9 2,686,890 + -1.4 2,686,891 - +2.5 2,686,946 + -0.2 2,687,112 + Sama_2223 0.17 -2.1 2,687,555 + Sama_2223 0.66 -0.7 2,687,555 + Sama_2223 0.66 -2.7 2,687,556 - Sama_2223 0.66 -1.0 2,687,612 + Sama_2223 0.72 -2.7 2,687,613 - Sama_2223 0.72 -2.2 2,687,772 + Sama_2223 0.90 -1.9 2,687,863 + +1.3 2,687,863 + -0.7 2,688,249 + Sama_2224 0.38 -2.5 2,688,528 + Sama_2224 0.65 +2.1 2,688,561 + Sama_2224 0.69 -2.5 2,688,639 + Sama_2224 0.76 -0.7 2,688,727 + Sama_2224 0.85 -2.5
Or see this region's nucleotide sequence