Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0620 and Dshi_0621 are separated by 159 nucleotides Dshi_0621 and Dshi_0622 are separated by 73 nucleotides Dshi_0622 and Dshi_0623 are separated by 345 nucleotides
Dshi_0620: Dshi_0620 - short-chain dehydrogenase/reductase SDR (RefSeq), at 612,860 to 613,612
_0620
Dshi_0621: Dshi_0621 - beta-Ig-H3/fasciclin (RefSeq), at 613,772 to 614,260
_0621
Dshi_0622: Dshi_0622 - beta-Ig-H3/fasciclin (RefSeq), at 614,334 to 614,825
_0622
Dshi_0623: Dshi_0623 - oxidoreductase molybdopterin binding (RefSeq), at 615,171 to 616,088
_0623
Position (kb)
614
615 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 613.343 kb on + strand, within Dshi_0620 at 613.356 kb on + strand, within Dshi_0620 at 613.435 kb on + strand, within Dshi_0620 at 613.468 kb on + strand, within Dshi_0620 at 613.468 kb on + strand, within Dshi_0620 at 613.476 kb on - strand, within Dshi_0620 at 613.478 kb on + strand, within Dshi_0620 at 613.591 kb on + strand at 613.709 kb on - strand at 613.710 kb on + strand at 613.715 kb on + strand at 613.723 kb on - strand at 613.819 kb on - strand at 613.887 kb on + strand, within Dshi_0621 at 613.887 kb on - strand, within Dshi_0621 at 613.924 kb on - strand, within Dshi_0621 at 613.931 kb on - strand, within Dshi_0621 at 613.941 kb on - strand, within Dshi_0621 at 613.967 kb on + strand, within Dshi_0621 at 614.001 kb on + strand, within Dshi_0621 at 614.012 kb on + strand, within Dshi_0621 at 614.012 kb on + strand, within Dshi_0621 at 614.208 kb on + strand, within Dshi_0621 at 614.218 kb on + strand at 614.311 kb on - strand at 614.317 kb on + strand at 614.356 kb on - strand at 614.408 kb on - strand, within Dshi_0622 at 614.423 kb on + strand, within Dshi_0622 at 614.448 kb on - strand, within Dshi_0622 at 614.532 kb on + strand, within Dshi_0622 at 614.555 kb on - strand, within Dshi_0622 at 614.631 kb on - strand, within Dshi_0622 at 614.739 kb on - strand, within Dshi_0622 at 614.750 kb on + strand, within Dshi_0622 at 614.768 kb on - strand, within Dshi_0622 at 614.779 kb on - strand at 614.792 kb on - strand at 614.864 kb on + strand at 614.886 kb on + strand at 614.894 kb on - strand at 614.894 kb on - strand at 614.922 kb on - strand at 614.964 kb on - strand at 614.973 kb on + strand at 614.983 kb on - strand at 614.990 kb on - strand at 615.032 kb on + strand at 615.074 kb on - strand at 615.074 kb on - strand at 615.110 kb on + strand at 615.116 kb on + strand at 615.124 kb on - strand at 615.147 kb on - strand at 615.181 kb on + strand at 615.198 kb on + strand at 615.198 kb on + strand at 615.198 kb on + strand at 615.199 kb on - strand at 615.206 kb on - strand at 615.391 kb on - strand, within Dshi_0623 at 615.409 kb on + strand, within Dshi_0623 at 615.409 kb on + strand, within Dshi_0623 at 615.410 kb on - strand, within Dshi_0623 at 615.414 kb on + strand, within Dshi_0623 at 615.444 kb on + strand, within Dshi_0623 at 615.464 kb on + strand, within Dshi_0623 at 615.535 kb on + strand, within Dshi_0623 at 615.535 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.537 kb on + strand, within Dshi_0623 at 615.545 kb on - strand, within Dshi_0623 at 615.545 kb on - strand, within Dshi_0623 at 615.545 kb on - strand, within Dshi_0623 at 615.594 kb on + strand, within Dshi_0623 at 615.638 kb on - strand, within Dshi_0623 at 615.652 kb on + strand, within Dshi_0623 at 615.708 kb on + strand, within Dshi_0623 at 615.716 kb on - strand, within Dshi_0623 at 615.733 kb on - strand, within Dshi_0623 at 615.775 kb on + strand, within Dshi_0623 at 615.792 kb on + strand, within Dshi_0623
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 613,343 + Dshi_0620 0.64 -0.6 613,356 + Dshi_0620 0.66 -2.5 613,435 + Dshi_0620 0.76 -0.8 613,468 + Dshi_0620 0.81 -0.4 613,468 + Dshi_0620 0.81 -1.7 613,476 - Dshi_0620 0.82 -1.3 613,478 + Dshi_0620 0.82 +0.3 613,591 + -0.2 613,709 - -0.1 613,710 + -0.1 613,715 + -0.5 613,723 - +0.7 613,819 - +0.5 613,887 + Dshi_0621 0.24 +1.0 613,887 - Dshi_0621 0.24 -0.2 613,924 - Dshi_0621 0.31 +0.1 613,931 - Dshi_0621 0.33 +0.3 613,941 - Dshi_0621 0.35 -0.0 613,967 + Dshi_0621 0.40 +0.5 614,001 + Dshi_0621 0.47 -0.0 614,012 + Dshi_0621 0.49 +0.7 614,012 + Dshi_0621 0.49 -0.5 614,208 + Dshi_0621 0.89 +0.0 614,218 + +0.8 614,311 - -0.8 614,317 + -0.1 614,356 - -0.4 614,408 - Dshi_0622 0.15 -0.3 614,423 + Dshi_0622 0.18 -0.8 614,448 - Dshi_0622 0.23 -0.5 614,532 + Dshi_0622 0.40 -0.1 614,555 - Dshi_0622 0.45 -0.5 614,631 - Dshi_0622 0.60 -0.8 614,739 - Dshi_0622 0.82 -0.9 614,750 + Dshi_0622 0.85 -1.6 614,768 - Dshi_0622 0.88 -0.1 614,779 - +1.2 614,792 - -2.8 614,864 + +0.8 614,886 + -2.1 614,894 - +0.6 614,894 - -0.1 614,922 - -0.5 614,964 - -0.6 614,973 + -0.4 614,983 - +0.1 614,990 - -0.9 615,032 + -0.7 615,074 - -0.1 615,074 - -0.3 615,110 + -0.6 615,116 + -0.5 615,124 - -0.2 615,147 - -0.3 615,181 + +0.4 615,198 + +0.1 615,198 + -0.1 615,198 + +0.7 615,199 - +0.2 615,206 - +0.3 615,391 - Dshi_0623 0.24 -0.3 615,409 + Dshi_0623 0.26 -0.8 615,409 + Dshi_0623 0.26 -0.3 615,410 - Dshi_0623 0.26 +1.2 615,414 + Dshi_0623 0.26 +0.5 615,444 + Dshi_0623 0.30 +0.4 615,464 + Dshi_0623 0.32 -0.2 615,535 + Dshi_0623 0.40 -0.4 615,535 + Dshi_0623 0.40 +1.9 615,537 + Dshi_0623 0.40 +1.2 615,537 + Dshi_0623 0.40 -0.2 615,537 + Dshi_0623 0.40 +0.3 615,537 + Dshi_0623 0.40 +1.6 615,537 + Dshi_0623 0.40 +0.1 615,537 + Dshi_0623 0.40 -1.0 615,537 + Dshi_0623 0.40 -1.7 615,537 + Dshi_0623 0.40 -0.9 615,537 + Dshi_0623 0.40 -1.0 615,545 - Dshi_0623 0.41 -1.3 615,545 - Dshi_0623 0.41 +1.9 615,545 - Dshi_0623 0.41 +1.4 615,594 + Dshi_0623 0.46 +0.3 615,638 - Dshi_0623 0.51 -0.0 615,652 + Dshi_0623 0.52 -0.3 615,708 + Dshi_0623 0.58 +2.0 615,716 - Dshi_0623 0.59 -0.8 615,733 - Dshi_0623 0.61 -0.6 615,775 + Dshi_0623 0.66 -0.6 615,792 + Dshi_0623 0.68 +1.5
Or see this region's nucleotide sequence