Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0329 and Dshi_0330 are separated by 47 nucleotides Dshi_0330 and Dshi_R0003 are separated by 171 nucleotides Dshi_R0003 and Dshi_0331 are separated by 6 nucleotides Dshi_0331 and Dshi_0332 overlap by 4 nucleotides Dshi_0332 and Dshi_0333 are separated by 62 nucleotides
Dshi_0329: Dshi_0329 - diguanylate cyclase/phosphodiesterase (RefSeq), at 317,056 to 318,645
_0329
Dshi_0330: Dshi_0330 - PP-loop domain protein (RefSeq), at 318,693 to 319,556
_0330
Dshi_R0003: Dshi_R0003 - tRNA-Thr (RefSeq), at 319,728 to 319,803
_R0003
Dshi_0331: Dshi_0331 - protein of unknown function DUF37 (RefSeq), at 319,810 to 320,031
_0331
Dshi_0332: Dshi_0332 - ribonuclease P protein component (RefSeq), at 320,028 to 320,417
_0332
Dshi_0333: Dshi_0333 - ribosomal protein L34 (RefSeq), at 320,480 to 320,614
_0333
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1 at 317.745 kb on - strand, within Dshi_0329 at 317.746 kb on + strand, within Dshi_0329 at 317.749 kb on - strand, within Dshi_0329 at 317.771 kb on + strand, within Dshi_0329 at 317.779 kb on - strand, within Dshi_0329 at 317.841 kb on - strand, within Dshi_0329 at 317.848 kb on - strand, within Dshi_0329 at 317.853 kb on - strand, within Dshi_0329 at 317.870 kb on - strand, within Dshi_0329 at 317.985 kb on + strand, within Dshi_0329 at 318.006 kb on + strand, within Dshi_0329 at 318.141 kb on + strand, within Dshi_0329 at 318.263 kb on + strand, within Dshi_0329 at 318.314 kb on + strand, within Dshi_0329 at 318.323 kb on - strand, within Dshi_0329 at 318.352 kb on - strand, within Dshi_0329 at 318.491 kb on + strand at 318.524 kb on - strand at 318.607 kb on + strand at 318.615 kb on - strand at 318.617 kb on - strand at 318.648 kb on + strand at 318.672 kb on + strand at 318.724 kb on + strand at 318.734 kb on + strand at 318.812 kb on + strand, within Dshi_0330 at 318.812 kb on + strand, within Dshi_0330 at 318.943 kb on + strand, within Dshi_0330 at 319.101 kb on - strand, within Dshi_0330 at 319.204 kb on - strand, within Dshi_0330 at 319.234 kb on + strand, within Dshi_0330 at 319.330 kb on + strand, within Dshi_0330 at 319.330 kb on + strand, within Dshi_0330 at 319.361 kb on + strand at 319.562 kb on - strand at 319.661 kb on + strand at 319.674 kb on - strand at 319.780 kb on - strand, within Dshi_R0003 at 319.872 kb on + strand, within Dshi_0331 at 319.953 kb on + strand, within Dshi_0331 at 319.983 kb on + strand, within Dshi_0331
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 317,745 - Dshi_0329 0.43 -1.2 317,746 + Dshi_0329 0.43 -0.1 317,749 - Dshi_0329 0.44 -2.6 317,771 + Dshi_0329 0.45 +0.5 317,779 - Dshi_0329 0.45 -0.9 317,841 - Dshi_0329 0.49 -0.8 317,848 - Dshi_0329 0.50 +0.0 317,853 - Dshi_0329 0.50 +1.4 317,870 - Dshi_0329 0.51 -1.0 317,985 + Dshi_0329 0.58 -0.4 318,006 + Dshi_0329 0.60 -0.4 318,141 + Dshi_0329 0.68 -1.0 318,263 + Dshi_0329 0.76 -0.1 318,314 + Dshi_0329 0.79 +0.2 318,323 - Dshi_0329 0.80 -0.9 318,352 - Dshi_0329 0.82 -0.1 318,491 + -2.0 318,524 - -0.8 318,607 + +1.1 318,615 - -0.7 318,617 - -0.5 318,648 + +0.2 318,672 + -0.2 318,724 + +0.4 318,734 + -1.0 318,812 + Dshi_0330 0.14 +0.2 318,812 + Dshi_0330 0.14 -1.2 318,943 + Dshi_0330 0.29 -0.4 319,101 - Dshi_0330 0.47 -0.7 319,204 - Dshi_0330 0.59 +0.3 319,234 + Dshi_0330 0.63 -1.2 319,330 + Dshi_0330 0.74 -0.3 319,330 + Dshi_0330 0.74 -0.3 319,361 + +0.7 319,562 - +0.1 319,661 + -0.2 319,674 - -0.0 319,780 - Dshi_R0003 0.68 +0.8 319,872 + Dshi_0331 0.28 -0.8 319,953 + Dshi_0331 0.64 -0.5 319,983 + Dshi_0331 0.78 +0.4
Or see this region's nucleotide sequence