Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0313 and Dshi_0314 are separated by 82 nucleotides Dshi_0314 and Dshi_0315 overlap by 4 nucleotides Dshi_0315 and Dshi_0316 overlap by 4 nucleotides Dshi_0316 and Dshi_0317 overlap by 4 nucleotides
Dshi_0313: Dshi_0313 - AAA ATPase central domain protein (RefSeq), at 301,799 to 303,109
_0313
Dshi_0314: Dshi_0314 - Camphor resistance CrcB protein (RefSeq), at 303,192 to 303,521
_0314
Dshi_0315: Dshi_0315 - pseudouridine synthase, RluA family (RefSeq), at 303,518 to 304,564
_0315
Dshi_0316: Dshi_0316 - HAD-superfamily hydrolase, subfamily IA, variant 3 (RefSeq), at 304,561 to 305,241
_0316
Dshi_0317: Dshi_0317 - ATP12 ATPase (RefSeq), at 305,238 to 305,942
_0317
Position (kb)
303
304
305 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 302.613 kb on - strand, within Dshi_0313 at 302.636 kb on - strand, within Dshi_0313 at 302.638 kb on - strand, within Dshi_0313 at 302.721 kb on + strand, within Dshi_0313 at 302.785 kb on + strand, within Dshi_0313 at 302.929 kb on + strand, within Dshi_0313 at 302.932 kb on - strand, within Dshi_0313 at 302.937 kb on + strand, within Dshi_0313 at 302.942 kb on + strand, within Dshi_0313 at 302.948 kb on + strand, within Dshi_0313 at 303.029 kb on + strand at 303.408 kb on - strand, within Dshi_0314 at 303.442 kb on + strand, within Dshi_0314 at 303.497 kb on - strand at 303.672 kb on - strand, within Dshi_0315 at 303.837 kb on - strand, within Dshi_0315 at 303.981 kb on - strand, within Dshi_0315 at 304.212 kb on - strand, within Dshi_0315 at 304.500 kb on + strand at 304.508 kb on - strand at 304.513 kb on + strand at 304.676 kb on - strand, within Dshi_0316 at 304.712 kb on - strand, within Dshi_0316 at 304.814 kb on + strand, within Dshi_0316 at 304.898 kb on - strand, within Dshi_0316 at 304.921 kb on + strand, within Dshi_0316 at 305.017 kb on - strand, within Dshi_0316 at 305.025 kb on + strand, within Dshi_0316 at 305.033 kb on - strand, within Dshi_0316 at 305.033 kb on - strand, within Dshi_0316 at 305.111 kb on + strand, within Dshi_0316 at 305.149 kb on - strand, within Dshi_0316 at 305.150 kb on + strand, within Dshi_0316 at 305.176 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 302,613 - Dshi_0313 0.62 -0.5 302,636 - Dshi_0313 0.64 -1.8 302,638 - Dshi_0313 0.64 -3.2 302,721 + Dshi_0313 0.70 +0.6 302,785 + Dshi_0313 0.75 +0.8 302,929 + Dshi_0313 0.86 +0.9 302,932 - Dshi_0313 0.86 -0.3 302,937 + Dshi_0313 0.87 +0.5 302,942 + Dshi_0313 0.87 +1.2 302,948 + Dshi_0313 0.88 +0.5 303,029 + +0.7 303,408 - Dshi_0314 0.65 -3.3 303,442 + Dshi_0314 0.76 +1.7 303,497 - -0.1 303,672 - Dshi_0315 0.15 -0.5 303,837 - Dshi_0315 0.30 -2.6 303,981 - Dshi_0315 0.44 -0.7 304,212 - Dshi_0315 0.66 -1.0 304,500 + -1.0 304,508 - -0.0 304,513 + -0.6 304,676 - Dshi_0316 0.17 +1.3 304,712 - Dshi_0316 0.22 -0.1 304,814 + Dshi_0316 0.37 +0.3 304,898 - Dshi_0316 0.49 +0.4 304,921 + Dshi_0316 0.53 -0.3 305,017 - Dshi_0316 0.67 +0.1 305,025 + Dshi_0316 0.68 +0.2 305,033 - Dshi_0316 0.69 -0.5 305,033 - Dshi_0316 0.69 +0.7 305,111 + Dshi_0316 0.81 +0.8 305,149 - Dshi_0316 0.86 +0.1 305,150 + Dshi_0316 0.86 +0.3 305,176 + +0.3
Or see this region's nucleotide sequence