Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0218 and Dshi_0219 are separated by 3 nucleotides Dshi_0219 and Dshi_0220 are separated by 84 nucleotides Dshi_0220 and Dshi_0221 are separated by 93 nucleotides
Dshi_0218: Dshi_0218 - ATP--cobalamin adenosyltransferase (RefSeq), at 216,003 to 216,575
_0218
Dshi_0219: Dshi_0219 - hypothetical protein (RefSeq), at 216,579 to 216,794
_0219
Dshi_0220: Dshi_0220 - short-chain dehydrogenase/reductase SDR (RefSeq), at 216,879 to 217,712
_0220
Dshi_0221: Dshi_0221 - DNA topoisomerase IV, A subunit (RefSeq), at 217,806 to 220,139
_0221
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 215.952 kb on - strand at 216.009 kb on - strand at 216.079 kb on - strand, within Dshi_0218 at 216.156 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.364 kb on - strand, within Dshi_0218 at 216.564 kb on + strand at 216.564 kb on + strand at 216.579 kb on - strand at 216.579 kb on - strand at 216.782 kb on - strand at 216.830 kb on + strand at 216.833 kb on - strand at 216.834 kb on + strand at 216.838 kb on - strand at 216.927 kb on - strand at 216.985 kb on - strand, within Dshi_0220 at 216.990 kb on - strand, within Dshi_0220 at 217.008 kb on - strand, within Dshi_0220 at 217.018 kb on + strand, within Dshi_0220 at 217.018 kb on + strand, within Dshi_0220 at 217.037 kb on + strand, within Dshi_0220 at 217.045 kb on - strand, within Dshi_0220 at 217.178 kb on + strand, within Dshi_0220 at 217.240 kb on - strand, within Dshi_0220 at 217.290 kb on - strand, within Dshi_0220 at 217.300 kb on - strand, within Dshi_0220 at 217.302 kb on + strand, within Dshi_0220 at 217.310 kb on - strand, within Dshi_0220 at 217.310 kb on - strand, within Dshi_0220 at 217.423 kb on + strand, within Dshi_0220 at 217.468 kb on + strand, within Dshi_0220 at 217.480 kb on + strand, within Dshi_0220 at 217.525 kb on + strand, within Dshi_0220 at 217.529 kb on + strand, within Dshi_0220 at 217.587 kb on - strand, within Dshi_0220 at 217.626 kb on - strand, within Dshi_0220 at 217.677 kb on + strand at 217.687 kb on + strand at 217.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 215,952 - -0.2 216,009 - -0.2 216,079 - Dshi_0218 0.13 -0.8 216,156 - Dshi_0218 0.27 -3.7 216,359 - Dshi_0218 0.62 -0.3 216,359 - Dshi_0218 0.62 -1.1 216,364 - Dshi_0218 0.63 +1.5 216,564 + -0.7 216,564 + -1.8 216,579 - -0.1 216,579 - -0.4 216,782 - -1.6 216,830 + +0.1 216,833 - +0.4 216,834 + +1.2 216,838 - -0.1 216,927 - -0.7 216,985 - Dshi_0220 0.13 -0.3 216,990 - Dshi_0220 0.13 -0.3 217,008 - Dshi_0220 0.15 -1.1 217,018 + Dshi_0220 0.17 -0.1 217,018 + Dshi_0220 0.17 -2.8 217,037 + Dshi_0220 0.19 +0.1 217,045 - Dshi_0220 0.20 -2.4 217,178 + Dshi_0220 0.36 -1.3 217,240 - Dshi_0220 0.43 +0.2 217,290 - Dshi_0220 0.49 -1.5 217,300 - Dshi_0220 0.50 -0.9 217,302 + Dshi_0220 0.51 +0.1 217,310 - Dshi_0220 0.52 -0.3 217,310 - Dshi_0220 0.52 +0.3 217,423 + Dshi_0220 0.65 -0.3 217,468 + Dshi_0220 0.71 +0.4 217,480 + Dshi_0220 0.72 -0.7 217,525 + Dshi_0220 0.77 +0.6 217,529 + Dshi_0220 0.78 -1.3 217,587 - Dshi_0220 0.85 -1.4 217,626 - Dshi_0220 0.90 +0.2 217,677 + +0.0 217,687 + +0.3 217,777 - +0.1
Or see this region's nucleotide sequence