Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0140 and Dshi_0141 overlap by 11 nucleotides Dshi_0141 and Dshi_0142 are separated by 163 nucleotides Dshi_0142 and Dshi_0143 are separated by 314 nucleotides Dshi_0143 and Dshi_0144 are separated by 34 nucleotides
Dshi_0140: Dshi_0140 - VacJ family lipoprotein (RefSeq), at 139,098 to 139,919
_0140
Dshi_0141: Dshi_0141 - toluene tolerance family protein (RefSeq), at 139,909 to 140,529
_0141
Dshi_0142: Dshi_0142 - penicillin-binding protein, 1A family (RefSeq), at 140,693 to 142,882
_0142
Dshi_0143: Dshi_0143 - nitrogen regulatory protein P-II (RefSeq), at 143,197 to 143,535
_0143
Dshi_0144: Dshi_0144 - ammonium transporter (RefSeq), at 143,570 to 144,883
_0144
Position (kb)
140
141
142
143 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 139.709 kb on - strand at 139.843 kb on + strand at 139.851 kb on - strand at 139.880 kb on + strand at 139.885 kb on + strand at 139.976 kb on + strand, within Dshi_0141 at 139.984 kb on - strand, within Dshi_0141 at 139.994 kb on - strand, within Dshi_0141 at 139.994 kb on - strand, within Dshi_0141 at 140.210 kb on + strand, within Dshi_0141 at 140.251 kb on - strand, within Dshi_0141 at 140.255 kb on + strand, within Dshi_0141 at 140.433 kb on - strand, within Dshi_0141 at 140.546 kb on - strand at 140.691 kb on + strand at 140.834 kb on - strand at 141.533 kb on - strand, within Dshi_0142 at 141.739 kb on - strand, within Dshi_0142 at 142.372 kb on + strand, within Dshi_0142 at 142.841 kb on - strand at 142.917 kb on + strand at 142.942 kb on - strand at 142.950 kb on - strand at 142.979 kb on - strand at 143.005 kb on - strand at 143.187 kb on + strand at 143.300 kb on - strand, within Dshi_0143 at 143.743 kb on + strand, within Dshi_0144 at 143.830 kb on - strand, within Dshi_0144
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 139,709 - +0.3 139,843 + -1.3 139,851 - +0.8 139,880 + -1.6 139,885 + -0.6 139,976 + Dshi_0141 0.11 -0.9 139,984 - Dshi_0141 0.12 -1.4 139,994 - Dshi_0141 0.14 -0.6 139,994 - Dshi_0141 0.14 -3.2 140,210 + Dshi_0141 0.48 -1.0 140,251 - Dshi_0141 0.55 -1.7 140,255 + Dshi_0141 0.56 -1.2 140,433 - Dshi_0141 0.84 +0.9 140,546 - -0.2 140,691 + +0.8 140,834 - -2.2 141,533 - Dshi_0142 0.38 -0.7 141,739 - Dshi_0142 0.48 +0.9 142,372 + Dshi_0142 0.77 +2.5 142,841 - -1.4 142,917 + +0.2 142,942 - -0.1 142,950 - +0.6 142,979 - +0.1 143,005 - -0.9 143,187 + -0.2 143,300 - Dshi_0143 0.30 +0.9 143,743 + Dshi_0144 0.13 -0.4 143,830 - Dshi_0144 0.20 +0.4
Or see this region's nucleotide sequence