Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagZ and SO2251 are separated by 81 nucleotides SO2251 and SO2253 are separated by 572 nucleotides SO2253 and SO2254 are separated by 120 nucleotides
SO2250: nagZ - beta-hexosaminidase (NCBI ptt file), at 2,364,234 to 2,365,262
nagZ
SO2251: SO2251 - conserved hypothetical protein (NCBI ptt file), at 2,365,344 to 2,365,883
SO2251
SO2253: SO2253 - acylphosphatase (NCBI ptt file), at 2,366,456 to 2,366,728
SO2253
SO2254: SO2254 - conserved hypothetical protein (NCBI ptt file), at 2,366,849 to 2,367,847
SO2254
Position (kb)
2365
2366 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2364.972 kb on - strand, within nagZ at 2365.297 kb on + strand at 2365.297 kb on + strand at 2365.527 kb on + strand, within SO2251 at 2365.744 kb on + strand, within SO2251 at 2365.754 kb on + strand, within SO2251 at 2365.875 kb on - strand at 2365.875 kb on - strand at 2365.885 kb on - strand at 2365.909 kb on - strand at 2366.024 kb on + strand at 2366.303 kb on - strand at 2366.407 kb on + strand at 2366.422 kb on + strand at 2366.489 kb on + strand, within SO2253 at 2366.494 kb on + strand, within SO2253 at 2366.661 kb on + strand, within SO2253 at 2366.762 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 2,364,972 - nagZ SO2250 0.72 +0.1 2,365,297 + +1.2 2,365,297 + -0.3 2,365,527 + SO2251 0.34 -1.8 2,365,744 + SO2251 0.74 -0.9 2,365,754 + SO2251 0.76 -0.4 2,365,875 - -0.8 2,365,875 - -0.1 2,365,885 - +0.3 2,365,909 - +1.0 2,366,024 + +0.4 2,366,303 - +2.0 2,366,407 + +0.4 2,366,422 + +0.4 2,366,489 + SO2253 0.12 -0.2 2,366,494 + SO2253 0.14 -0.2 2,366,661 + SO2253 0.75 +0.1 2,366,762 + +1.3
Or see this region's nucleotide sequence