Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1894 and SO1895 overlap by 1 nucleotides SO1895 and pccB-1 are separated by 46 nucleotides pccB-1 and ivd are separated by 116 nucleotides
SO1894: SO1894 - acetyl-CoA carboxylase, biotin carboxylase, putative (NCBI ptt file), at 1,990,428 to 1,992,512
SO1894
SO1895: SO1895 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,992,512 to 1,993,378
SO1895
SO1896: pccB-1 - 3-methylcrotonyl CoA carboxylase, beta subunit (NCBI ptt file), at 1,993,425 to 1,995,032
pccB-1
SO1897: ivd - isovaleryl-CoA dehydrogenase (NCBI ptt file), at 1,995,149 to 1,996,318
ivd
Position (kb)
1993
1994
1995
1996 Strain fitness (log2 ratio)
-2
-1
0
1 at 1992.427 kb on - strand at 1992.495 kb on + strand at 1992.584 kb on + strand at 1992.663 kb on + strand, within SO1895 at 1992.826 kb on + strand, within SO1895 at 1992.968 kb on + strand, within SO1895 at 1992.980 kb on + strand, within SO1895 at 1992.980 kb on + strand, within SO1895 at 1993.056 kb on + strand, within SO1895 at 1993.228 kb on + strand, within SO1895 at 1993.261 kb on + strand, within SO1895 at 1993.312 kb on + strand at 1993.355 kb on + strand at 1993.583 kb on + strand at 1993.890 kb on - strand, within pccB-1 at 1993.892 kb on + strand, within pccB-1 at 1993.892 kb on + strand, within pccB-1 at 1994.079 kb on - strand, within pccB-1 at 1994.261 kb on + strand, within pccB-1 at 1994.269 kb on - strand, within pccB-1 at 1994.269 kb on - strand, within pccB-1 at 1994.469 kb on + strand, within pccB-1 at 1994.472 kb on - strand, within pccB-1 at 1994.477 kb on - strand, within pccB-1 at 1994.503 kb on + strand, within pccB-1 at 1994.927 kb on - strand at 1995.137 kb on + strand at 1995.142 kb on + strand at 1995.169 kb on - strand at 1995.204 kb on - strand at 1995.218 kb on + strand at 1995.218 kb on + strand at 1995.419 kb on + strand, within ivd at 1995.427 kb on - strand, within ivd at 1995.427 kb on - strand, within ivd at 1995.427 kb on - strand, within ivd at 1995.563 kb on - strand, within ivd at 1995.651 kb on + strand, within ivd at 1995.681 kb on - strand, within ivd at 1995.681 kb on - strand, within ivd at 1995.776 kb on - strand, within ivd at 1995.987 kb on + strand, within ivd
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 1,992,427 - -1.0 1,992,495 + +0.6 1,992,584 + -0.4 1,992,663 + SO1895 0.17 -1.7 1,992,826 + SO1895 0.36 +0.3 1,992,968 + SO1895 0.53 -1.5 1,992,980 + SO1895 0.54 -1.9 1,992,980 + SO1895 0.54 -1.8 1,993,056 + SO1895 0.63 -1.6 1,993,228 + SO1895 0.83 -1.2 1,993,261 + SO1895 0.86 -1.5 1,993,312 + -2.7 1,993,355 + -0.4 1,993,583 + -1.4 1,993,890 - pccB-1 SO1896 0.29 -2.2 1,993,892 + pccB-1 SO1896 0.29 -1.9 1,993,892 + pccB-1 SO1896 0.29 -2.1 1,994,079 - pccB-1 SO1896 0.41 -2.4 1,994,261 + pccB-1 SO1896 0.52 -1.4 1,994,269 - pccB-1 SO1896 0.52 -2.3 1,994,269 - pccB-1 SO1896 0.52 -2.7 1,994,469 + pccB-1 SO1896 0.65 -0.4 1,994,472 - pccB-1 SO1896 0.65 -2.2 1,994,477 - pccB-1 SO1896 0.65 -2.3 1,994,503 + pccB-1 SO1896 0.67 -1.7 1,994,927 - -2.2 1,995,137 + -2.5 1,995,142 + -0.7 1,995,169 - -0.8 1,995,204 - -1.9 1,995,218 + -0.6 1,995,218 + -0.1 1,995,419 + ivd SO1897 0.23 -0.2 1,995,427 - ivd SO1897 0.24 +0.4 1,995,427 - ivd SO1897 0.24 -0.4 1,995,427 - ivd SO1897 0.24 -0.4 1,995,563 - ivd SO1897 0.35 -0.7 1,995,651 + ivd SO1897 0.43 -0.4 1,995,681 - ivd SO1897 0.45 -0.9 1,995,681 - ivd SO1897 0.45 +0.5 1,995,776 - ivd SO1897 0.54 -0.4 1,995,987 + ivd SO1897 0.72 -0.5
Or see this region's nucleotide sequence