Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panB and folK-1 are separated by 56 nucleotides folK-1 and pcnB overlap by 1 nucleotides pcnB and SO0873 are separated by 274 nucleotides
SO0870: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI ptt file), at 900,169 to 900,963
panB
SO0871: folK-1 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 901,020 to 901,508
folK-1
SO0872: pcnB - polyA polymerase (NCBI ptt file), at 901,508 to 902,824
pcnB
SO0873: SO0873 - glutamyl-tRNA synthetase domain protein (NCBI ptt file), at 903,099 to 903,998
SO0873
Position (kb) 901
902
903 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 900.551 kb on - strand, within panB at 900.740 kb on - strand, within panB at 900.800 kb on - strand, within panB at 900.947 kb on - strand at 900.962 kb on - strand at 901.040 kb on - strand at 901.074 kb on + strand, within folK-1 at 901.571 kb on - strand at 901.752 kb on - strand, within pcnB at 902.032 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.358 kb on - strand, within pcnB at 902.714 kb on - strand at 902.744 kb on - strand at 902.750 kb on + strand at 902.833 kb on - strand at 902.868 kb on - strand at 902.896 kb on - strand at 903.055 kb on - strand at 903.055 kb on - strand at 903.134 kb on - strand at 903.207 kb on + strand, within SO0873 at 903.216 kb on + strand, within SO0873 at 903.227 kb on + strand, within SO0873 at 903.438 kb on - strand, within SO0873 at 903.467 kb on + strand, within SO0873 at 903.591 kb on + strand, within SO0873 at 903.721 kb on + strand, within SO0873 at 903.751 kb on + strand, within SO0873 at 903.768 kb on + strand, within SO0873 at 903.784 kb on - strand, within SO0873
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 900,551 - panB SO0870 0.48 -4.4 900,740 - panB SO0870 0.72 -1.1 900,800 - panB SO0870 0.79 -3.6 900,947 - -1.1 900,962 - -3.9 901,040 - -2.3 901,074 + folK-1 SO0871 0.11 -3.5 901,571 - -2.1 901,752 - pcnB SO0872 0.19 -1.4 902,032 - pcnB SO0872 0.40 -0.7 902,288 - pcnB SO0872 0.59 +1.2 902,288 - pcnB SO0872 0.59 -2.5 902,343 - pcnB SO0872 0.63 -3.5 902,343 - pcnB SO0872 0.63 -2.1 902,343 - pcnB SO0872 0.63 -1.4 902,343 - pcnB SO0872 0.63 -2.1 902,343 - pcnB SO0872 0.63 -1.4 902,343 - pcnB SO0872 0.63 -1.6 902,343 - pcnB SO0872 0.63 -1.8 902,343 - pcnB SO0872 0.63 -0.8 902,343 - pcnB SO0872 0.63 -1.1 902,343 - pcnB SO0872 0.63 -1.3 902,358 - pcnB SO0872 0.65 -1.3 902,714 - -1.8 902,744 - -2.7 902,750 + +0.4 902,833 - +0.0 902,868 - -4.2 902,896 - -3.1 903,055 - +0.1 903,055 - -0.2 903,134 - +0.0 903,207 + SO0873 0.12 +0.0 903,216 + SO0873 0.13 -1.0 903,227 + SO0873 0.14 +0.6 903,438 - SO0873 0.38 +0.6 903,467 + SO0873 0.41 -0.1 903,591 + SO0873 0.55 +0.6 903,721 + SO0873 0.69 -0.3 903,751 + SO0873 0.72 -0.4 903,768 + SO0873 0.74 +1.4 903,784 - SO0873 0.76 -1.1
Or see this region's nucleotide sequence