Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemE and SO0437 are separated by 659 nucleotides SO0437 and SO0438 are separated by 75 nucleotides SO0438 and SO0439 overlap by 4 nucleotides
SO0435: hemE - uroporphyrinogen decarboxylase (NCBI ptt file), at 459,487 to 460,551
hemE
SO0437: SO0437 - sensory box protein (NCBI ptt file), at 461,211 to 463,781
SO0437
SO0438: SO0438 - oxidoreductase, short chain dehydrogenase/reductase family (NCBI ptt file), at 463,857 to 464,660
SO0438
SO0439: SO0439 - hypothetical protein (NCBI ptt file), at 464,657 to 464,932
SO0439
Position (kb) 461
462
463
464 Strain fitness (log2 ratio) -2
-1
0
1
2 at 460.546 kb on + strand at 460.550 kb on + strand at 460.581 kb on - strand at 460.604 kb on - strand at 460.610 kb on + strand at 461.055 kb on - strand at 461.079 kb on + strand at 461.099 kb on + strand at 461.174 kb on - strand at 461.252 kb on + strand at 461.278 kb on + strand at 461.284 kb on + strand at 461.418 kb on + strand at 461.426 kb on - strand at 461.435 kb on - strand at 461.484 kb on - strand, within SO0437 at 461.484 kb on - strand, within SO0437 at 461.500 kb on + strand, within SO0437 at 461.500 kb on + strand, within SO0437 at 461.508 kb on - strand, within SO0437 at 461.508 kb on - strand, within SO0437 at 461.556 kb on + strand, within SO0437 at 461.593 kb on - strand, within SO0437 at 461.593 kb on - strand, within SO0437 at 461.600 kb on + strand, within SO0437 at 461.600 kb on + strand, within SO0437 at 461.608 kb on - strand, within SO0437 at 461.709 kb on - strand at 461.756 kb on - strand, within SO0437 at 461.756 kb on - strand, within SO0437 at 461.778 kb on + strand, within SO0437 at 461.878 kb on + strand, within SO0437 at 461.948 kb on - strand, within SO0437 at 461.981 kb on - strand, within SO0437 at 462.139 kb on + strand, within SO0437 at 462.217 kb on - strand, within SO0437 at 462.219 kb on - strand, within SO0437 at 462.243 kb on - strand, within SO0437 at 462.340 kb on - strand, within SO0437 at 462.582 kb on - strand, within SO0437 at 462.582 kb on - strand, within SO0437 at 462.622 kb on - strand, within SO0437 at 462.625 kb on + strand, within SO0437 at 462.712 kb on + strand, within SO0437 at 462.737 kb on + strand, within SO0437 at 462.771 kb on - strand, within SO0437 at 462.818 kb on - strand, within SO0437 at 462.858 kb on - strand, within SO0437 at 462.888 kb on - strand, within SO0437 at 463.002 kb on + strand, within SO0437 at 463.029 kb on - strand, within SO0437 at 463.098 kb on - strand, within SO0437 at 463.171 kb on - strand, within SO0437 at 463.183 kb on + strand, within SO0437 at 463.191 kb on - strand, within SO0437 at 463.430 kb on + strand, within SO0437 at 463.475 kb on + strand, within SO0437 at 463.577 kb on + strand at 463.577 kb on + strand at 463.774 kb on + strand at 463.775 kb on - strand at 463.791 kb on + strand at 463.810 kb on + strand at 463.990 kb on + strand, within SO0438 at 463.998 kb on - strand, within SO0438 at 464.053 kb on + strand, within SO0438 at 464.149 kb on + strand, within SO0438 at 464.258 kb on + strand, within SO0438 at 464.260 kb on + strand, within SO0438 at 464.291 kb on + strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.456 kb on + strand, within SO0438 at 464.481 kb on + strand, within SO0438 at 464.511 kb on - strand, within SO0438 at 464.593 kb on + strand at 464.601 kb on - strand at 464.601 kb on - strand at 464.646 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 460,546 + +0.4 460,550 + +1.1 460,581 - -0.1 460,604 - -0.4 460,610 + -0.4 461,055 - -0.1 461,079 + +0.9 461,099 + +1.6 461,174 - +0.6 461,252 + -1.6 461,278 + +0.3 461,284 + -1.1 461,418 + +0.6 461,426 - -0.1 461,435 - +1.0 461,484 - SO0437 0.11 +0.6 461,484 - SO0437 0.11 -0.1 461,500 + SO0437 0.11 -0.0 461,500 + SO0437 0.11 +0.0 461,508 - SO0437 0.12 -0.0 461,508 - SO0437 0.12 -0.6 461,556 + SO0437 0.13 -1.4 461,593 - SO0437 0.15 -0.1 461,593 - SO0437 0.15 +2.3 461,600 + SO0437 0.15 +0.4 461,600 + SO0437 0.15 +0.4 461,608 - SO0437 0.15 -0.1 461,709 - +0.0 461,756 - SO0437 0.21 -0.1 461,756 - SO0437 0.21 +0.1 461,778 + SO0437 0.22 -0.7 461,878 + SO0437 0.26 -0.4 461,948 - SO0437 0.29 -0.8 461,981 - SO0437 0.30 +0.0 462,139 + SO0437 0.36 +2.4 462,217 - SO0437 0.39 +0.4 462,219 - SO0437 0.39 +1.4 462,243 - SO0437 0.40 +0.3 462,340 - SO0437 0.44 +1.2 462,582 - SO0437 0.53 +1.3 462,582 - SO0437 0.53 +0.9 462,622 - SO0437 0.55 -0.6 462,625 + SO0437 0.55 +0.3 462,712 + SO0437 0.58 -0.7 462,737 + SO0437 0.59 +0.4 462,771 - SO0437 0.61 +0.3 462,818 - SO0437 0.63 +0.7 462,858 - SO0437 0.64 -0.2 462,888 - SO0437 0.65 -0.0 463,002 + SO0437 0.70 +1.4 463,029 - SO0437 0.71 -2.1 463,098 - SO0437 0.73 -0.5 463,171 - SO0437 0.76 -0.6 463,183 + SO0437 0.77 +1.4 463,191 - SO0437 0.77 +0.8 463,430 + SO0437 0.86 +0.6 463,475 + SO0437 0.88 +0.2 463,577 + -0.8 463,577 + -1.7 463,774 + -0.1 463,775 - +0.1 463,791 + -0.6 463,810 + -0.9 463,990 + SO0438 0.17 +1.3 463,998 - SO0438 0.18 +1.4 464,053 + SO0438 0.24 -0.1 464,149 + SO0438 0.36 +2.1 464,258 + SO0438 0.50 +2.0 464,260 + SO0438 0.50 +0.7 464,291 + SO0438 0.54 +0.7 464,299 - SO0438 0.55 -0.2 464,299 - SO0438 0.55 +0.2 464,299 - SO0438 0.55 -0.3 464,299 - SO0438 0.55 +0.4 464,456 + SO0438 0.75 +0.6 464,481 + SO0438 0.78 -1.6 464,511 - SO0438 0.81 +0.8 464,593 + -0.3 464,601 - +1.2 464,601 - +0.4 464,646 + -0.1
Or see this region's nucleotide sequence