Experiment: L-Glutamic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4199 and Dshi_4200 are separated by 145 nucleotides Dshi_4200 and Dshi_4201 overlap by 4 nucleotides Dshi_4201 and Dshi_4202 overlap by 4 nucleotides
Dshi_4199: Dshi_4199 - transcriptional regulator, TetR family (RefSeq), at 45,953 to 46,597
_4199
Dshi_4200: Dshi_4200 - Carbon-monoxide dehydrogenase (acceptor) (RefSeq), at 46,743 to 47,576
_4200
Dshi_4201: Dshi_4201 - (2Fe-2S)-binding domain protein (RefSeq), at 47,573 to 48,073
_4201
Dshi_4202: Dshi_4202 - Carbon-monoxide dehydrogenase (acceptor) (RefSeq), at 48,070 to 50,451
_4202
Position (kb) 47
48
49 Strain fitness (log2 ratio) -1
0
1 at 46.771 kb on + strand at 46.982 kb on + strand, within Dshi_4200 at 47.297 kb on + strand, within Dshi_4200 at 47.316 kb on - strand, within Dshi_4200 at 47.331 kb on + strand, within Dshi_4200 at 47.589 kb on - strand at 47.630 kb on + strand, within Dshi_4201 at 47.638 kb on + strand, within Dshi_4201 at 47.638 kb on - strand, within Dshi_4201 at 47.651 kb on + strand at 47.690 kb on - strand, within Dshi_4201 at 47.816 kb on + strand, within Dshi_4201 at 48.159 kb on - strand at 48.231 kb on - strand at 48.328 kb on + strand, within Dshi_4202 at 48.348 kb on - strand, within Dshi_4202
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (C) remove 46,771 + -0.2 46,982 + Dshi_4200 0.29 +0.8 47,297 + Dshi_4200 0.66 -1.0 47,316 - Dshi_4200 0.69 -1.2 47,331 + Dshi_4200 0.71 +0.2 47,589 - -0.2 47,630 + Dshi_4201 0.11 +0.1 47,638 + Dshi_4201 0.13 +1.3 47,638 - Dshi_4201 0.13 +0.7 47,651 + +0.1 47,690 - Dshi_4201 0.23 +0.2 47,816 + Dshi_4201 0.49 -0.2 48,159 - +0.6 48,231 - +0.0 48,328 + Dshi_4202 0.11 -0.5 48,348 - Dshi_4202 0.12 -0.0
Or see this region's nucleotide sequence