Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4718 and SO4719 are separated by 283 nucleotides SO4719 and SO4720 overlap by 4 nucleotides SO4720 and SO4721 are separated by 3 nucleotides
SO4718: SO4718 - sigma-54 dependent response regulator (NCBI ptt file), at 4,922,525 to 4,923,907
SO4718
SO4719: SO4719 - conserved hypothetical protein (NCBI ptt file), at 4,924,191 to 4,925,015
SO4719
SO4720: SO4720 - ABC transporter, permease protein (NCBI ptt file), at 4,925,012 to 4,925,719
SO4720
SO4721: SO4721 - ABC transporter, ATP-binding protein (NCBI ptt file), at 4,925,723 to 4,926,430
SO4721
Position (kb)
4924
4925
4926 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4923.306 kb on - strand, within SO4718 at 4923.349 kb on - strand, within SO4718 at 4923.376 kb on - strand, within SO4718 at 4923.474 kb on + strand, within SO4718 at 4923.484 kb on + strand, within SO4718 at 4923.492 kb on - strand, within SO4718 at 4923.492 kb on - strand, within SO4718 at 4923.517 kb on + strand, within SO4718 at 4923.522 kb on - strand, within SO4718 at 4923.522 kb on - strand, within SO4718 at 4923.555 kb on - strand, within SO4718 at 4923.672 kb on + strand, within SO4718 at 4923.691 kb on - strand, within SO4718 at 4923.691 kb on - strand, within SO4718 at 4923.695 kb on - strand, within SO4718 at 4923.762 kb on - strand, within SO4718 at 4923.771 kb on - strand at 4923.771 kb on - strand at 4923.780 kb on + strand at 4923.901 kb on + strand at 4923.901 kb on + strand at 4923.901 kb on + strand at 4923.924 kb on - strand at 4924.032 kb on + strand at 4924.143 kb on - strand at 4924.437 kb on + strand, within SO4719 at 4924.444 kb on + strand, within SO4719 at 4924.489 kb on + strand, within SO4719 at 4924.489 kb on + strand, within SO4719 at 4924.490 kb on - strand, within SO4719 at 4924.638 kb on - strand, within SO4719 at 4924.694 kb on + strand, within SO4719 at 4924.694 kb on + strand, within SO4719 at 4924.702 kb on - strand, within SO4719 at 4924.714 kb on - strand, within SO4719 at 4924.716 kb on + strand, within SO4719 at 4924.762 kb on + strand, within SO4719 at 4924.762 kb on + strand, within SO4719 at 4924.768 kb on - strand, within SO4719 at 4924.770 kb on - strand, within SO4719 at 4924.770 kb on - strand, within SO4719 at 4924.770 kb on - strand, within SO4719 at 4924.774 kb on - strand, within SO4719 at 4924.853 kb on + strand, within SO4719 at 4924.901 kb on + strand, within SO4719 at 4924.942 kb on - strand at 4924.966 kb on + strand at 4924.991 kb on + strand at 4924.991 kb on + strand at 4924.991 kb on + strand at 4924.999 kb on - strand at 4925.033 kb on - strand at 4925.033 kb on - strand at 4925.121 kb on - strand, within SO4720 at 4925.133 kb on + strand, within SO4720 at 4925.133 kb on - strand, within SO4720 at 4925.141 kb on - strand, within SO4720 at 4925.141 kb on - strand, within SO4720 at 4925.150 kb on + strand, within SO4720 at 4925.158 kb on - strand, within SO4720 at 4925.245 kb on - strand, within SO4720 at 4925.327 kb on - strand, within SO4720 at 4925.386 kb on - strand, within SO4720 at 4925.386 kb on - strand, within SO4720 at 4925.386 kb on - strand, within SO4720 at 4925.421 kb on + strand, within SO4720 at 4925.421 kb on + strand, within SO4720 at 4925.421 kb on + strand, within SO4720 at 4925.427 kb on - strand, within SO4720 at 4925.429 kb on - strand, within SO4720 at 4925.429 kb on - strand, within SO4720 at 4925.468 kb on - strand, within SO4720 at 4925.530 kb on - strand, within SO4720 at 4925.556 kb on + strand, within SO4720 at 4925.637 kb on + strand, within SO4720 at 4925.645 kb on - strand, within SO4720 at 4925.645 kb on - strand, within SO4720 at 4925.671 kb on - strand at 4925.720 kb on + strand at 4925.789 kb on + strand at 4925.797 kb on - strand, within SO4721 at 4925.797 kb on - strand, within SO4721 at 4925.834 kb on + strand, within SO4721 at 4925.842 kb on + strand, within SO4721 at 4925.861 kb on - strand, within SO4721 at 4925.861 kb on - strand, within SO4721 at 4925.907 kb on - strand, within SO4721
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,923,306 - SO4718 0.56 +1.0 4,923,349 - SO4718 0.60 +1.5 4,923,376 - SO4718 0.62 -0.7 4,923,474 + SO4718 0.69 +0.7 4,923,484 + SO4718 0.69 -1.3 4,923,492 - SO4718 0.70 +1.2 4,923,492 - SO4718 0.70 +1.2 4,923,517 + SO4718 0.72 +1.0 4,923,522 - SO4718 0.72 +1.4 4,923,522 - SO4718 0.72 -0.5 4,923,555 - SO4718 0.74 +1.6 4,923,672 + SO4718 0.83 +0.7 4,923,691 - SO4718 0.84 +0.4 4,923,691 - SO4718 0.84 +2.6 4,923,695 - SO4718 0.85 +0.4 4,923,762 - SO4718 0.89 +2.4 4,923,771 - +1.0 4,923,771 - +2.0 4,923,780 + +1.7 4,923,901 + +1.0 4,923,901 + +1.2 4,923,901 + +1.7 4,923,924 - -0.6 4,924,032 + +2.0 4,924,143 - +2.1 4,924,437 + SO4719 0.30 -1.8 4,924,444 + SO4719 0.31 +0.8 4,924,489 + SO4719 0.36 +1.8 4,924,489 + SO4719 0.36 +0.3 4,924,490 - SO4719 0.36 +1.4 4,924,638 - SO4719 0.54 +2.2 4,924,694 + SO4719 0.61 +0.5 4,924,694 + SO4719 0.61 +1.3 4,924,702 - SO4719 0.62 +0.5 4,924,714 - SO4719 0.63 +1.1 4,924,716 + SO4719 0.64 +1.2 4,924,762 + SO4719 0.69 +1.4 4,924,762 + SO4719 0.69 +1.5 4,924,768 - SO4719 0.70 +1.6 4,924,770 - SO4719 0.70 +1.1 4,924,770 - SO4719 0.70 -0.6 4,924,770 - SO4719 0.70 +1.4 4,924,774 - SO4719 0.71 +1.6 4,924,853 + SO4719 0.80 +0.3 4,924,901 + SO4719 0.86 +1.1 4,924,942 - +1.0 4,924,966 + +0.2 4,924,991 + -1.4 4,924,991 + -0.1 4,924,991 + +0.5 4,924,999 - +1.6 4,925,033 - +0.7 4,925,033 - +0.3 4,925,121 - SO4720 0.15 +2.0 4,925,133 + SO4720 0.17 +2.4 4,925,133 - SO4720 0.17 +1.7 4,925,141 - SO4720 0.18 +0.6 4,925,141 - SO4720 0.18 +0.9 4,925,150 + SO4720 0.19 +0.7 4,925,158 - SO4720 0.21 -0.1 4,925,245 - SO4720 0.33 +0.0 4,925,327 - SO4720 0.44 +1.7 4,925,386 - SO4720 0.53 +0.9 4,925,386 - SO4720 0.53 +1.2 4,925,386 - SO4720 0.53 +1.4 4,925,421 + SO4720 0.58 +0.9 4,925,421 + SO4720 0.58 +1.1 4,925,421 + SO4720 0.58 +1.8 4,925,427 - SO4720 0.59 +0.2 4,925,429 - SO4720 0.59 +0.5 4,925,429 - SO4720 0.59 +1.6 4,925,468 - SO4720 0.64 -0.3 4,925,530 - SO4720 0.73 +1.5 4,925,556 + SO4720 0.77 +2.2 4,925,637 + SO4720 0.88 +0.7 4,925,645 - SO4720 0.89 +2.2 4,925,645 - SO4720 0.89 -0.2 4,925,671 - +1.2 4,925,720 + +0.0 4,925,789 + +1.1 4,925,797 - SO4721 0.10 +0.0 4,925,797 - SO4721 0.10 +1.0 4,925,834 + SO4721 0.16 -0.7 4,925,842 + SO4721 0.17 +1.0 4,925,861 - SO4721 0.19 +1.3 4,925,861 - SO4721 0.19 +0.6 4,925,907 - SO4721 0.26 +0.1
Or see this region's nucleotide sequence