Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4511 and SO4512 are separated by 379 nucleotides SO4512 and SO4513 are separated by 17 nucleotides SO4513 and fdhB-2 are separated by 29 nucleotides fdhB-2 and SO4515 are separated by 76 nucleotides
SO4511: SO4511 - formate dehydrogenase, C subunit, putative (NCBI ptt file), at 4,703,999 to 4,705,027
SO4511
SO4512: SO4512 - conserved hypothetical protein (NCBI ptt file), at 4,705,407 to 4,705,607
SO4512
SO4513: SO4513 - formate dehydrogenase, alpha subunit (NCBI ptt file), at 4,705,625 to 4,708,477
SO4513
SO4514: fdhB-2 - formate dehydrogenase, iron-sulfur subunit (NCBI ptt file), at 4,708,507 to 4,709,076
fdhB-2
SO4515: SO4515 - formate dehydrogenase, C subunit, putative (NCBI ptt file), at 4,709,153 to 4,710,136
SO4515
Position (kb)
4705
4706
4707
4708
4709 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4704.626 kb on - strand, within SO4511 at 4704.626 kb on - strand, within SO4511 at 4704.651 kb on - strand, within SO4511 at 4704.679 kb on + strand, within SO4511 at 4704.776 kb on - strand, within SO4511 at 4704.798 kb on + strand, within SO4511 at 4704.798 kb on + strand, within SO4511 at 4704.798 kb on + strand, within SO4511 at 4704.798 kb on + strand, within SO4511 at 4704.803 kb on + strand, within SO4511 at 4704.803 kb on + strand, within SO4511 at 4704.806 kb on - strand, within SO4511 at 4704.806 kb on - strand, within SO4511 at 4704.811 kb on - strand, within SO4511 at 4704.826 kb on - strand, within SO4511 at 4704.842 kb on + strand, within SO4511 at 4704.879 kb on - strand, within SO4511 at 4704.925 kb on - strand at 4704.934 kb on - strand at 4705.048 kb on + strand at 4705.280 kb on + strand at 4705.280 kb on + strand at 4705.419 kb on - strand at 4705.509 kb on + strand, within SO4512 at 4705.518 kb on + strand, within SO4512 at 4705.545 kb on - strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.547 kb on + strand, within SO4512 at 4705.555 kb on - strand, within SO4512 at 4705.555 kb on - strand, within SO4512 at 4705.555 kb on - strand, within SO4512 at 4705.574 kb on + strand, within SO4512 at 4705.641 kb on + strand at 4705.687 kb on - strand at 4705.752 kb on + strand at 4705.785 kb on - strand at 4705.785 kb on - strand at 4705.853 kb on - strand at 4705.873 kb on - strand at 4705.886 kb on + strand at 4705.886 kb on + strand at 4705.886 kb on + strand at 4705.886 kb on + strand at 4705.886 kb on + strand at 4705.886 kb on + strand at 4705.887 kb on + strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.894 kb on - strand at 4705.940 kb on - strand, within SO4513 at 4705.940 kb on - strand, within SO4513 at 4705.949 kb on - strand, within SO4513 at 4706.109 kb on - strand, within SO4513 at 4706.112 kb on + strand, within SO4513 at 4706.119 kb on + strand, within SO4513 at 4706.134 kb on - strand, within SO4513 at 4706.144 kb on - strand, within SO4513 at 4706.247 kb on - strand, within SO4513 at 4706.262 kb on + strand, within SO4513 at 4706.304 kb on + strand, within SO4513 at 4706.304 kb on + strand, within SO4513 at 4706.313 kb on - strand, within SO4513 at 4706.519 kb on + strand, within SO4513 at 4706.527 kb on - strand, within SO4513 at 4706.642 kb on + strand, within SO4513 at 4706.642 kb on + strand, within SO4513 at 4706.671 kb on + strand, within SO4513 at 4706.797 kb on + strand, within SO4513 at 4706.847 kb on + strand, within SO4513 at 4706.852 kb on + strand, within SO4513 at 4706.860 kb on - strand, within SO4513 at 4706.876 kb on + strand, within SO4513 at 4706.880 kb on + strand, within SO4513 at 4706.884 kb on - strand, within SO4513 at 4706.893 kb on - strand, within SO4513 at 4706.942 kb on - strand, within SO4513 at 4706.988 kb on + strand, within SO4513 at 4707.047 kb on + strand, within SO4513 at 4707.081 kb on - strand, within SO4513 at 4707.244 kb on - strand, within SO4513 at 4707.246 kb on + strand, within SO4513 at 4707.246 kb on + strand, within SO4513 at 4707.339 kb on + strand, within SO4513 at 4707.437 kb on - strand, within SO4513 at 4707.450 kb on + strand, within SO4513 at 4707.458 kb on - strand, within SO4513 at 4707.458 kb on - strand, within SO4513 at 4707.458 kb on - strand, within SO4513 at 4707.458 kb on - strand, within SO4513 at 4707.458 kb on - strand, within SO4513 at 4707.541 kb on - strand, within SO4513 at 4707.541 kb on - strand, within SO4513 at 4707.547 kb on - strand, within SO4513 at 4707.553 kb on - strand, within SO4513 at 4707.580 kb on - strand, within SO4513 at 4707.609 kb on + strand, within SO4513 at 4707.609 kb on + strand, within SO4513 at 4707.761 kb on - strand, within SO4513 at 4707.870 kb on + strand, within SO4513 at 4707.878 kb on - strand, within SO4513 at 4707.915 kb on - strand, within SO4513 at 4707.923 kb on + strand, within SO4513 at 4707.923 kb on + strand, within SO4513 at 4707.923 kb on + strand, within SO4513 at 4707.932 kb on + strand, within SO4513 at 4707.932 kb on + strand, within SO4513 at 4708.027 kb on + strand, within SO4513 at 4708.027 kb on + strand, within SO4513 at 4708.027 kb on + strand, within SO4513 at 4708.027 kb on + strand, within SO4513 at 4708.035 kb on - strand, within SO4513 at 4708.035 kb on - strand, within SO4513 at 4708.088 kb on - strand, within SO4513 at 4708.131 kb on + strand, within SO4513 at 4708.131 kb on + strand, within SO4513 at 4708.179 kb on - strand, within SO4513 at 4708.241 kb on - strand at 4708.261 kb on + strand at 4708.264 kb on - strand at 4708.296 kb on + strand at 4708.304 kb on - strand at 4708.304 kb on - strand at 4708.362 kb on + strand at 4708.400 kb on + strand at 4708.405 kb on + strand at 4708.408 kb on - strand at 4708.448 kb on + strand at 4708.491 kb on - strand at 4708.491 kb on - strand at 4708.534 kb on + strand at 4708.562 kb on + strand at 4708.685 kb on + strand, within fdhB-2 at 4708.693 kb on - strand, within fdhB-2 at 4708.753 kb on - strand, within fdhB-2 at 4708.880 kb on - strand, within fdhB-2 at 4708.954 kb on + strand, within fdhB-2 at 4708.962 kb on - strand, within fdhB-2 at 4708.962 kb on - strand, within fdhB-2 at 4708.962 kb on - strand, within fdhB-2 at 4708.964 kb on + strand, within fdhB-2 at 4708.964 kb on + strand, within fdhB-2 at 4708.972 kb on - strand, within fdhB-2 at 4709.081 kb on + strand at 4709.109 kb on - strand at 4709.177 kb on + strand at 4709.181 kb on - strand at 4709.185 kb on - strand at 4709.185 kb on - strand at 4709.192 kb on + strand at 4709.197 kb on + strand at 4709.234 kb on - strand at 4709.276 kb on + strand, within SO4515 at 4709.278 kb on + strand, within SO4515 at 4709.278 kb on + strand, within SO4515 at 4709.278 kb on + strand, within SO4515 at 4709.318 kb on - strand, within SO4515 at 4709.343 kb on + strand, within SO4515 at 4709.343 kb on + strand, within SO4515 at 4709.343 kb on + strand, within SO4515 at 4709.343 kb on - strand, within SO4515 at 4709.351 kb on - strand, within SO4515 at 4709.358 kb on + strand, within SO4515 at 4709.358 kb on + strand, within SO4515 at 4709.379 kb on + strand, within SO4515 at 4709.455 kb on + strand, within SO4515
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,704,626 - SO4511 0.61 +1.0 4,704,626 - SO4511 0.61 +0.5 4,704,651 - SO4511 0.63 -0.1 4,704,679 + SO4511 0.66 -0.3 4,704,776 - SO4511 0.76 -0.7 4,704,798 + SO4511 0.78 +0.8 4,704,798 + SO4511 0.78 +1.6 4,704,798 + SO4511 0.78 +1.3 4,704,798 + SO4511 0.78 -0.3 4,704,803 + SO4511 0.78 +1.7 4,704,803 + SO4511 0.78 +0.2 4,704,806 - SO4511 0.78 +1.0 4,704,806 - SO4511 0.78 +0.9 4,704,811 - SO4511 0.79 +0.1 4,704,826 - SO4511 0.80 +2.3 4,704,842 + SO4511 0.82 -0.5 4,704,879 - SO4511 0.86 +2.1 4,704,925 - +0.5 4,704,934 - -0.6 4,705,048 + -0.5 4,705,280 + +0.5 4,705,280 + -0.3 4,705,419 - +0.4 4,705,509 + SO4512 0.51 +1.4 4,705,518 + SO4512 0.55 -1.0 4,705,545 - SO4512 0.69 +1.4 4,705,547 + SO4512 0.70 -0.3 4,705,547 + SO4512 0.70 +0.0 4,705,547 + SO4512 0.70 +0.7 4,705,547 + SO4512 0.70 -0.6 4,705,547 + SO4512 0.70 +1.0 4,705,547 + SO4512 0.70 -1.2 4,705,547 + SO4512 0.70 -2.7 4,705,547 + SO4512 0.70 -0.0 4,705,547 + SO4512 0.70 +2.3 4,705,547 + SO4512 0.70 +0.5 4,705,547 + SO4512 0.70 -1.9 4,705,555 - SO4512 0.74 -0.2 4,705,555 - SO4512 0.74 +1.2 4,705,555 - SO4512 0.74 +1.5 4,705,574 + SO4512 0.83 +0.3 4,705,641 + -1.4 4,705,687 - +0.5 4,705,752 + +1.3 4,705,785 - +1.5 4,705,785 - -1.7 4,705,853 - -2.2 4,705,873 - +0.1 4,705,886 + -1.0 4,705,886 + -0.6 4,705,886 + -0.0 4,705,886 + -1.3 4,705,886 + -1.3 4,705,886 + -0.2 4,705,887 + +0.4 4,705,894 - +0.1 4,705,894 - +0.9 4,705,894 - -0.9 4,705,894 - -0.1 4,705,894 - +0.9 4,705,894 - -0.6 4,705,894 - +0.1 4,705,894 - +0.3 4,705,894 - -0.3 4,705,940 - SO4513 0.11 +1.3 4,705,940 - SO4513 0.11 +0.9 4,705,949 - SO4513 0.11 +0.8 4,706,109 - SO4513 0.17 -0.6 4,706,112 + SO4513 0.17 +0.3 4,706,119 + SO4513 0.17 +1.6 4,706,134 - SO4513 0.18 -0.7 4,706,144 - SO4513 0.18 +0.9 4,706,247 - SO4513 0.22 +1.3 4,706,262 + SO4513 0.22 -3.2 4,706,304 + SO4513 0.24 -0.9 4,706,304 + SO4513 0.24 -1.6 4,706,313 - SO4513 0.24 +0.3 4,706,519 + SO4513 0.31 +0.6 4,706,527 - SO4513 0.32 -2.8 4,706,642 + SO4513 0.36 -1.6 4,706,642 + SO4513 0.36 -0.2 4,706,671 + SO4513 0.37 -0.3 4,706,797 + SO4513 0.41 -1.2 4,706,847 + SO4513 0.43 -0.3 4,706,852 + SO4513 0.43 +0.1 4,706,860 - SO4513 0.43 +0.1 4,706,876 + SO4513 0.44 -0.1 4,706,880 + SO4513 0.44 -1.6 4,706,884 - SO4513 0.44 +2.2 4,706,893 - SO4513 0.44 -0.0 4,706,942 - SO4513 0.46 +0.7 4,706,988 + SO4513 0.48 -1.9 4,707,047 + SO4513 0.50 +0.1 4,707,081 - SO4513 0.51 -0.5 4,707,244 - SO4513 0.57 -0.0 4,707,246 + SO4513 0.57 +0.0 4,707,246 + SO4513 0.57 -1.1 4,707,339 + SO4513 0.60 +0.0 4,707,437 - SO4513 0.64 -0.4 4,707,450 + SO4513 0.64 -0.4 4,707,458 - SO4513 0.64 +0.9 4,707,458 - SO4513 0.64 +0.3 4,707,458 - SO4513 0.64 -0.2 4,707,458 - SO4513 0.64 -1.9 4,707,458 - SO4513 0.64 -0.2 4,707,541 - SO4513 0.67 +1.2 4,707,541 - SO4513 0.67 +1.0 4,707,547 - SO4513 0.67 -0.0 4,707,553 - SO4513 0.68 +0.5 4,707,580 - SO4513 0.69 +0.3 4,707,609 + SO4513 0.70 -0.8 4,707,609 + SO4513 0.70 +0.1 4,707,761 - SO4513 0.75 +0.0 4,707,870 + SO4513 0.79 -0.5 4,707,878 - SO4513 0.79 -1.6 4,707,915 - SO4513 0.80 -0.3 4,707,923 + SO4513 0.81 -0.6 4,707,923 + SO4513 0.81 -0.6 4,707,923 + SO4513 0.81 -0.3 4,707,932 + SO4513 0.81 -0.7 4,707,932 + SO4513 0.81 -0.6 4,708,027 + SO4513 0.84 -1.0 4,708,027 + SO4513 0.84 -0.5 4,708,027 + SO4513 0.84 -0.5 4,708,027 + SO4513 0.84 -2.6 4,708,035 - SO4513 0.84 +0.3 4,708,035 - SO4513 0.84 -2.0 4,708,088 - SO4513 0.86 -1.8 4,708,131 + SO4513 0.88 -1.8 4,708,131 + SO4513 0.88 +1.2 4,708,179 - SO4513 0.90 -0.1 4,708,241 - +0.2 4,708,261 + +4.2 4,708,264 - -0.2 4,708,296 + -1.0 4,708,304 - -2.2 4,708,304 - +0.2 4,708,362 + -0.1 4,708,400 + +0.5 4,708,405 + -0.2 4,708,408 - -0.4 4,708,448 + +1.7 4,708,491 - +0.2 4,708,491 - -0.3 4,708,534 + +0.9 4,708,562 + +0.7 4,708,685 + fdhB-2 SO4514 0.31 +0.1 4,708,693 - fdhB-2 SO4514 0.33 +0.6 4,708,753 - fdhB-2 SO4514 0.43 +0.8 4,708,880 - fdhB-2 SO4514 0.65 -1.7 4,708,954 + fdhB-2 SO4514 0.78 -1.2 4,708,962 - fdhB-2 SO4514 0.80 +0.6 4,708,962 - fdhB-2 SO4514 0.80 -1.3 4,708,962 - fdhB-2 SO4514 0.80 -0.3 4,708,964 + fdhB-2 SO4514 0.80 -0.6 4,708,964 + fdhB-2 SO4514 0.80 +0.9 4,708,972 - fdhB-2 SO4514 0.82 -0.5 4,709,081 + -1.5 4,709,109 - +1.2 4,709,177 + +0.7 4,709,181 - +1.2 4,709,185 - +0.6 4,709,185 - -0.8 4,709,192 + -0.3 4,709,197 + -0.0 4,709,234 - +0.7 4,709,276 + SO4515 0.12 -0.6 4,709,278 + SO4515 0.13 -1.4 4,709,278 + SO4515 0.13 +0.3 4,709,278 + SO4515 0.13 -1.1 4,709,318 - SO4515 0.17 -1.1 4,709,343 + SO4515 0.19 +0.4 4,709,343 + SO4515 0.19 -0.4 4,709,343 + SO4515 0.19 +0.4 4,709,343 - SO4515 0.19 +0.1 4,709,351 - SO4515 0.20 -1.2 4,709,358 + SO4515 0.21 -1.4 4,709,358 + SO4515 0.21 -0.3 4,709,379 + SO4515 0.23 +0.1 4,709,455 + SO4515 0.31 +0.7
Or see this region's nucleotide sequence