Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3964 and ptsO overlap by 14 nucleotides ptsO and mgtE-2 are separated by 196 nucleotides mgtE-2 and SO3967 are separated by 82 nucleotides
SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191
SO3964
SO3965: ptsO - phosphocarrier protein NPR (NCBI ptt file), at 4,105,178 to 4,105,453
ptsO
SO3966: mgtE-2 - magnesium transporter (NCBI ptt file), at 4,105,650 to 4,107,014
mgtE-2
SO3967: SO3967 - molybdenum ABC transporter, periplasmic molybdenum-binding protein, putative (NCBI ptt file), at 4,107,097 to 4,107,870
SO3967
Position (kb) 4105
4106
4107
4108 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4104.942 kb on - strand, within SO3964 at 4105.286 kb on - strand, within ptsO at 4105.421 kb on + strand, within ptsO at 4105.443 kb on - strand at 4105.654 kb on - strand at 4105.680 kb on - strand at 4105.906 kb on + strand, within mgtE-2 at 4105.914 kb on - strand, within mgtE-2 at 4106.006 kb on + strand, within mgtE-2 at 4106.048 kb on + strand, within mgtE-2 at 4106.056 kb on - strand, within mgtE-2 at 4106.082 kb on - strand, within mgtE-2 at 4106.165 kb on - strand, within mgtE-2 at 4106.223 kb on - strand, within mgtE-2 at 4106.223 kb on - strand, within mgtE-2 at 4106.496 kb on + strand, within mgtE-2 at 4106.498 kb on + strand, within mgtE-2 at 4106.505 kb on + strand, within mgtE-2 at 4106.505 kb on + strand, within mgtE-2 at 4106.505 kb on + strand, within mgtE-2 at 4106.505 kb on + strand, within mgtE-2 at 4106.527 kb on - strand, within mgtE-2 at 4106.530 kb on + strand, within mgtE-2 at 4106.530 kb on + strand, within mgtE-2 at 4106.793 kb on + strand, within mgtE-2 at 4106.793 kb on + strand, within mgtE-2 at 4106.839 kb on + strand, within mgtE-2 at 4106.844 kb on - strand, within mgtE-2 at 4106.847 kb on - strand, within mgtE-2 at 4106.940 kb on + strand at 4107.009 kb on + strand at 4107.127 kb on - strand at 4107.219 kb on - strand, within SO3967 at 4107.262 kb on - strand, within SO3967 at 4107.351 kb on - strand, within SO3967 at 4107.351 kb on - strand, within SO3967 at 4107.369 kb on + strand, within SO3967 at 4107.369 kb on + strand, within SO3967 at 4107.386 kb on - strand, within SO3967 at 4107.397 kb on - strand, within SO3967 at 4107.428 kb on + strand, within SO3967 at 4107.594 kb on + strand, within SO3967 at 4107.615 kb on - strand, within SO3967 at 4107.733 kb on - strand, within SO3967 at 4107.803 kb on - strand at 4107.814 kb on + strand at 4107.841 kb on - strand at 4107.860 kb on + strand at 4107.865 kb on + strand at 4107.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,104,942 - SO3964 0.71 -0.3 4,105,286 - ptsO SO3965 0.39 +1.3 4,105,421 + ptsO SO3965 0.88 +1.7 4,105,443 - +1.7 4,105,654 - -0.7 4,105,680 - -2.2 4,105,906 + mgtE-2 SO3966 0.19 -0.6 4,105,914 - mgtE-2 SO3966 0.19 -0.8 4,106,006 + mgtE-2 SO3966 0.26 -1.7 4,106,048 + mgtE-2 SO3966 0.29 -0.0 4,106,056 - mgtE-2 SO3966 0.30 -0.1 4,106,082 - mgtE-2 SO3966 0.32 +0.5 4,106,165 - mgtE-2 SO3966 0.38 -0.8 4,106,223 - mgtE-2 SO3966 0.42 -0.6 4,106,223 - mgtE-2 SO3966 0.42 -1.0 4,106,496 + mgtE-2 SO3966 0.62 +0.4 4,106,498 + mgtE-2 SO3966 0.62 -1.3 4,106,505 + mgtE-2 SO3966 0.63 +0.1 4,106,505 + mgtE-2 SO3966 0.63 -0.8 4,106,505 + mgtE-2 SO3966 0.63 -1.0 4,106,505 + mgtE-2 SO3966 0.63 -2.2 4,106,527 - mgtE-2 SO3966 0.64 -3.2 4,106,530 + mgtE-2 SO3966 0.64 -1.9 4,106,530 + mgtE-2 SO3966 0.64 -3.4 4,106,793 + mgtE-2 SO3966 0.84 -1.4 4,106,793 + mgtE-2 SO3966 0.84 -0.8 4,106,839 + mgtE-2 SO3966 0.87 -1.6 4,106,844 - mgtE-2 SO3966 0.87 -0.7 4,106,847 - mgtE-2 SO3966 0.88 -1.8 4,106,940 + -1.8 4,107,009 + -1.6 4,107,127 - -2.5 4,107,219 - SO3967 0.16 -1.1 4,107,262 - SO3967 0.21 -0.1 4,107,351 - SO3967 0.33 +0.1 4,107,351 - SO3967 0.33 +0.1 4,107,369 + SO3967 0.35 -0.8 4,107,369 + SO3967 0.35 -0.0 4,107,386 - SO3967 0.37 +1.9 4,107,397 - SO3967 0.39 +1.0 4,107,428 + SO3967 0.43 +1.7 4,107,594 + SO3967 0.64 -0.7 4,107,615 - SO3967 0.67 +0.4 4,107,733 - SO3967 0.82 -0.8 4,107,803 - +0.9 4,107,814 + +1.1 4,107,841 - +0.1 4,107,860 + -2.8 4,107,865 + +1.5 4,107,921 - +0.6
Or see this region's nucleotide sequence