Strain Fitness in Shewanella oneidensis MR-1 around SO3227

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliI and fliH overlap by 32 nucleotidesfliH and fliG overlap by 14 nucleotidesfliG and fliF overlap by 8 nucleotides SO3225: fliI - flagellum-specific ATP synthase FliI (NCBI ptt file), at 3,363,288 to 3,364,625 fliI SO3226: fliH - flagellar assembly protein FliH (NCBI ptt file), at 3,364,594 to 3,365,544 fliH SO3227: fliG - flagellar motor switch protein FliG (NCBI ptt file), at 3,365,531 to 3,366,577 fliG SO3228: fliF - flagellar M-ring protein FliF (NCBI ptt file), at 3,366,570 to 3,368,231 fliF Position (kb) 3365 3366 3367Strain fitness (log2 ratio) -1 0 1 2 3 4at 3364.553 kb on - strandat 3364.553 kb on - strandat 3365.035 kb on - strand, within fliHat 3365.071 kb on - strand, within fliHat 3365.076 kb on + strand, within fliHat 3365.084 kb on - strand, within fliHat 3365.084 kb on - strand, within fliHat 3365.113 kb on - strand, within fliHat 3365.204 kb on - strand, within fliHat 3365.239 kb on + strand, within fliHat 3365.246 kb on + strand, within fliHat 3365.249 kb on - strand, within fliHat 3365.264 kb on - strand, within fliHat 3365.292 kb on + strand, within fliHat 3365.299 kb on - strand, within fliHat 3365.300 kb on - strand, within fliHat 3365.417 kb on - strand, within fliHat 3365.417 kb on - strand, within fliHat 3365.583 kb on + strandat 3365.644 kb on - strand, within fliGat 3365.744 kb on - strand, within fliGat 3365.777 kb on - strand, within fliGat 3365.783 kb on + strand, within fliGat 3365.791 kb on - strand, within fliGat 3365.926 kb on - strand, within fliGat 3365.972 kb on + strand, within fliGat 3365.980 kb on - strand, within fliGat 3366.027 kb on + strand, within fliGat 3366.035 kb on - strand, within fliGat 3366.266 kb on + strand, within fliGat 3366.274 kb on - strand, within fliGat 3366.292 kb on - strand, within fliGat 3366.393 kb on - strand, within fliGat 3366.430 kb on - strand, within fliGat 3366.432 kb on + strand, within fliGat 3366.439 kb on + strand, within fliGat 3366.475 kb on + strandat 3366.646 kb on + strandat 3366.654 kb on - strandat 3366.694 kb on - strandat 3366.708 kb on + strandat 3366.716 kb on - strandat 3366.716 kb on - strandat 3366.737 kb on + strand, within fliFat 3366.737 kb on + strand, within fliFat 3366.744 kb on + strand, within fliFat 3366.745 kb on - strand, within fliFat 3366.745 kb on - strand, within fliFat 3366.745 kb on - strand, within fliFat 3366.764 kb on - strand, within fliFat 3366.849 kb on - strand, within fliFat 3366.988 kb on + strand, within fliFat 3367.003 kb on - strand, within fliFat 3367.030 kb on - strand, within fliFat 3367.081 kb on - strand, within fliFat 3367.123 kb on - strand, within fliFat 3367.123 kb on - strand, within fliFat 3367.186 kb on - strand, within fliF

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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3,364,553 - +0.9
3,364,553 - +1.2
3,365,035 - fliH SO3226 0.46 +1.5
3,365,071 - fliH SO3226 0.50 +0.5
3,365,076 + fliH SO3226 0.51 +1.9
3,365,084 - fliH SO3226 0.52 +0.3
3,365,084 - fliH SO3226 0.52 -0.5
3,365,113 - fliH SO3226 0.55 +0.7
3,365,204 - fliH SO3226 0.64 -0.1
3,365,239 + fliH SO3226 0.68 +0.7
3,365,246 + fliH SO3226 0.69 +0.9
3,365,249 - fliH SO3226 0.69 +1.0
3,365,264 - fliH SO3226 0.70 +1.0
3,365,292 + fliH SO3226 0.73 +2.6
3,365,299 - fliH SO3226 0.74 +1.4
3,365,300 - fliH SO3226 0.74 +0.4
3,365,417 - fliH SO3226 0.87 +4.0
3,365,417 - fliH SO3226 0.87 +1.2
3,365,583 + +2.9
3,365,644 - fliG SO3227 0.11 +2.1
3,365,744 - fliG SO3227 0.20 +3.7
3,365,777 - fliG SO3227 0.23 +3.3
3,365,783 + fliG SO3227 0.24 +4.2
3,365,791 - fliG SO3227 0.25 +0.9
3,365,926 - fliG SO3227 0.38 +2.5
3,365,972 + fliG SO3227 0.42 +3.4
3,365,980 - fliG SO3227 0.43 +3.5
3,366,027 + fliG SO3227 0.47 +3.5
3,366,035 - fliG SO3227 0.48 +3.1
3,366,266 + fliG SO3227 0.70 +4.3
3,366,274 - fliG SO3227 0.71 +4.5
3,366,292 - fliG SO3227 0.73 +3.6
3,366,393 - fliG SO3227 0.82 +3.0
3,366,430 - fliG SO3227 0.86 +0.5
3,366,432 + fliG SO3227 0.86 +4.0
3,366,439 + fliG SO3227 0.87 +3.7
3,366,475 + +3.5
3,366,646 + +3.6
3,366,654 - +2.6
3,366,694 - +2.8
3,366,708 + +3.5
3,366,716 - +2.7
3,366,716 - +3.0
3,366,737 + fliF SO3228 0.10 +3.5
3,366,737 + fliF SO3228 0.10 +3.0
3,366,744 + fliF SO3228 0.10 +3.5
3,366,745 - fliF SO3228 0.11 +2.4
3,366,745 - fliF SO3228 0.11 +3.7
3,366,745 - fliF SO3228 0.11 +4.0
3,366,764 - fliF SO3228 0.12 +2.0
3,366,849 - fliF SO3228 0.17 +2.5
3,366,988 + fliF SO3228 0.25 +4.4
3,367,003 - fliF SO3228 0.26 +2.5
3,367,030 - fliF SO3228 0.28 +3.0
3,367,081 - fliF SO3228 0.31 +3.6
3,367,123 - fliF SO3228 0.33 +2.3
3,367,123 - fliF SO3228 0.33 +3.0
3,367,186 - fliF SO3228 0.37 +3.0

Or see this region's nucleotide sequence