Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppsA and SO2645 are separated by 125 nucleotides SO2645 and aroH are separated by 182 nucleotides
SO2644: ppsA - phosphoenolpyruvate synthase (NCBI ptt file), at 2,779,864 to 2,782,233
ppsA
SO2645: SO2645 - conserved hypothetical protein (NCBI ptt file), at 2,782,359 to 2,783,171
SO2645
SO2646: aroH - phospho-2-dehydro-3-deoxyheptonate aldolase, trp-sensitive (NCBI ptt file), at 2,783,354 to 2,784,418
aroH
Position (kb)
2782
2783
2784 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2781.560 kb on + strand, within ppsA at 2781.709 kb on + strand, within ppsA at 2781.812 kb on + strand, within ppsA at 2781.863 kb on + strand, within ppsA at 2781.952 kb on - strand, within ppsA at 2781.952 kb on - strand, within ppsA at 2781.997 kb on - strand at 2782.225 kb on + strand at 2782.402 kb on + strand at 2782.418 kb on - strand at 2782.652 kb on + strand, within SO2645 at 2782.677 kb on + strand, within SO2645 at 2782.677 kb on + strand, within SO2645 at 2782.677 kb on + strand, within SO2645 at 2782.677 kb on + strand, within SO2645 at 2782.677 kb on + strand, within SO2645 at 2782.685 kb on - strand, within SO2645 at 2782.685 kb on - strand, within SO2645 at 2782.685 kb on - strand, within SO2645 at 2782.685 kb on - strand, within SO2645 at 2782.685 kb on - strand, within SO2645 at 2782.748 kb on + strand, within SO2645 at 2782.748 kb on + strand, within SO2645 at 2782.756 kb on - strand, within SO2645 at 2782.783 kb on - strand, within SO2645 at 2782.792 kb on + strand, within SO2645 at 2782.792 kb on + strand, within SO2645 at 2782.845 kb on - strand, within SO2645 at 2782.906 kb on + strand, within SO2645 at 2782.906 kb on + strand, within SO2645 at 2782.906 kb on + strand, within SO2645 at 2782.906 kb on + strand, within SO2645 at 2782.906 kb on + strand, within SO2645 at 2782.906 kb on + strand, within SO2645 at 2782.914 kb on - strand, within SO2645 at 2782.914 kb on - strand, within SO2645 at 2782.914 kb on - strand, within SO2645 at 2782.914 kb on - strand, within SO2645 at 2782.914 kb on - strand, within SO2645 at 2783.012 kb on - strand, within SO2645 at 2783.036 kb on - strand, within SO2645 at 2783.157 kb on - strand at 2783.181 kb on + strand at 2783.189 kb on - strand at 2783.189 kb on - strand at 2783.191 kb on + strand at 2783.280 kb on + strand at 2783.280 kb on + strand at 2783.280 kb on + strand at 2783.280 kb on + strand at 2783.288 kb on - strand at 2783.288 kb on - strand at 2783.288 kb on - strand at 2783.367 kb on - strand at 2783.367 kb on - strand at 2783.409 kb on - strand at 2783.480 kb on + strand, within aroH at 2783.583 kb on - strand, within aroH at 2783.758 kb on + strand, within aroH at 2783.859 kb on + strand, within aroH at 2783.867 kb on - strand, within aroH at 2783.867 kb on - strand, within aroH at 2783.887 kb on - strand, within aroH at 2784.131 kb on - strand, within aroH
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,781,560 + ppsA SO2644 0.72 -2.6 2,781,709 + ppsA SO2644 0.78 -3.6 2,781,812 + ppsA SO2644 0.82 -4.0 2,781,863 + ppsA SO2644 0.84 -3.4 2,781,952 - ppsA SO2644 0.88 -1.8 2,781,952 - ppsA SO2644 0.88 -1.8 2,781,997 - -4.0 2,782,225 + -1.4 2,782,402 + -1.4 2,782,418 - -3.1 2,782,652 + SO2645 0.36 -5.9 2,782,677 + SO2645 0.39 -2.2 2,782,677 + SO2645 0.39 -2.6 2,782,677 + SO2645 0.39 -0.8 2,782,677 + SO2645 0.39 -3.7 2,782,677 + SO2645 0.39 -2.5 2,782,685 - SO2645 0.40 -1.0 2,782,685 - SO2645 0.40 -1.7 2,782,685 - SO2645 0.40 -0.2 2,782,685 - SO2645 0.40 -1.6 2,782,685 - SO2645 0.40 +0.8 2,782,748 + SO2645 0.48 -0.3 2,782,748 + SO2645 0.48 -1.2 2,782,756 - SO2645 0.49 -1.4 2,782,783 - SO2645 0.52 +3.0 2,782,792 + SO2645 0.53 -0.1 2,782,792 + SO2645 0.53 -0.4 2,782,845 - SO2645 0.60 -1.5 2,782,906 + SO2645 0.67 +1.0 2,782,906 + SO2645 0.67 +0.1 2,782,906 + SO2645 0.67 -0.2 2,782,906 + SO2645 0.67 +0.4 2,782,906 + SO2645 0.67 -1.4 2,782,906 + SO2645 0.67 +0.2 2,782,914 - SO2645 0.68 -0.8 2,782,914 - SO2645 0.68 -1.2 2,782,914 - SO2645 0.68 -0.2 2,782,914 - SO2645 0.68 +0.2 2,782,914 - SO2645 0.68 -0.6 2,783,012 - SO2645 0.80 -1.4 2,783,036 - SO2645 0.83 +1.0 2,783,157 - -0.8 2,783,181 + -0.7 2,783,189 - +1.8 2,783,189 - -0.3 2,783,191 + +0.4 2,783,280 + -0.8 2,783,280 + -3.0 2,783,280 + -2.0 2,783,280 + +0.0 2,783,288 - +0.8 2,783,288 - -1.8 2,783,288 - -1.9 2,783,367 - -1.6 2,783,367 - -0.2 2,783,409 - -0.5 2,783,480 + aroH SO2646 0.12 -1.1 2,783,583 - aroH SO2646 0.22 +0.2 2,783,758 + aroH SO2646 0.38 +0.1 2,783,859 + aroH SO2646 0.47 -1.6 2,783,867 - aroH SO2646 0.48 +0.4 2,783,867 - aroH SO2646 0.48 +1.5 2,783,887 - aroH SO2646 0.50 +0.5 2,784,131 - aroH SO2646 0.73 -2.5
Or see this region's nucleotide sequence