Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemB-1 and SO2588 are separated by 713 nucleotides SO2588 and SO2589 are separated by 378 nucleotides
SO2587: hemB-1 - delta-aminolevulinic acid dehydratase (NCBI ptt file), at 2,713,793 to 2,714,794
hemB-1
SO2588: SO2588 - protein-methionine-S-oxide reductase, PilB family (NCBI ptt file), at 2,715,508 to 2,716,413
SO2588
SO2589: SO2589 - oxidoreductase, iron/ascorbate family (NCBI ptt file), at 2,716,792 to 2,717,631
SO2589
Position (kb)
2715
2716
2717 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2714.511 kb on - strand, within hemB-1 at 2714.673 kb on + strand, within hemB-1 at 2714.673 kb on + strand, within hemB-1 at 2714.746 kb on + strand at 2714.801 kb on + strand at 2714.852 kb on - strand at 2715.549 kb on + strand at 2715.794 kb on - strand, within SO2588 at 2715.815 kb on + strand, within SO2588 at 2715.968 kb on + strand, within SO2588 at 2715.968 kb on + strand, within SO2588 at 2715.976 kb on - strand, within SO2588 at 2715.976 kb on - strand, within SO2588 at 2716.056 kb on - strand, within SO2588 at 2716.097 kb on - strand, within SO2588 at 2716.097 kb on - strand, within SO2588 at 2716.319 kb on - strand, within SO2588 at 2716.455 kb on - strand at 2716.455 kb on - strand at 2716.570 kb on + strand at 2716.624 kb on + strand at 2716.624 kb on + strand at 2716.649 kb on - strand at 2716.661 kb on - strand at 2716.661 kb on - strand at 2716.661 kb on - strand at 2716.690 kb on - strand at 2716.843 kb on + strand at 2716.885 kb on - strand, within SO2589 at 2716.914 kb on + strand, within SO2589 at 2716.914 kb on + strand, within SO2589 at 2716.914 kb on + strand, within SO2589 at 2716.914 kb on + strand, within SO2589 at 2716.922 kb on - strand, within SO2589 at 2716.922 kb on - strand, within SO2589 at 2716.922 kb on - strand, within SO2589 at 2717.035 kb on + strand, within SO2589 at 2717.041 kb on - strand, within SO2589 at 2717.052 kb on + strand, within SO2589 at 2717.107 kb on - strand, within SO2589 at 2717.253 kb on + strand, within SO2589 at 2717.261 kb on - strand, within SO2589 at 2717.345 kb on - strand, within SO2589
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,714,511 - hemB-1 SO2587 0.72 +0.2 2,714,673 + hemB-1 SO2587 0.88 -1.0 2,714,673 + hemB-1 SO2587 0.88 +0.4 2,714,746 + -0.7 2,714,801 + -0.7 2,714,852 - -0.7 2,715,549 + -1.8 2,715,794 - SO2588 0.32 -4.2 2,715,815 + SO2588 0.34 -0.8 2,715,968 + SO2588 0.51 -2.4 2,715,968 + SO2588 0.51 -2.6 2,715,976 - SO2588 0.52 -4.7 2,715,976 - SO2588 0.52 -2.3 2,716,056 - SO2588 0.60 -3.5 2,716,097 - SO2588 0.65 -2.7 2,716,097 - SO2588 0.65 -1.8 2,716,319 - SO2588 0.90 -6.2 2,716,455 - +0.9 2,716,455 - -0.3 2,716,570 + +2.2 2,716,624 + +0.3 2,716,624 + +1.0 2,716,649 - +0.9 2,716,661 - +2.5 2,716,661 - +0.3 2,716,661 - -0.5 2,716,690 - +0.4 2,716,843 + +0.8 2,716,885 - SO2589 0.11 +1.9 2,716,914 + SO2589 0.15 +1.8 2,716,914 + SO2589 0.15 +0.5 2,716,914 + SO2589 0.15 +0.1 2,716,914 + SO2589 0.15 +0.4 2,716,922 - SO2589 0.15 -1.3 2,716,922 - SO2589 0.15 +1.5 2,716,922 - SO2589 0.15 -0.2 2,717,035 + SO2589 0.29 +0.2 2,717,041 - SO2589 0.30 -0.1 2,717,052 + SO2589 0.31 -0.5 2,717,107 - SO2589 0.38 -0.4 2,717,253 + SO2589 0.55 +0.2 2,717,261 - SO2589 0.56 +0.0 2,717,345 - SO2589 0.66 +0.4
Or see this region's nucleotide sequence