Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt def-3 and SO2532 are separated by 293 nucleotides SO2532 and SO2533 are separated by 129 nucleotides SO2533 and SO2535 are separated by 139 nucleotides
SO2530: def-3 - polypeptide deformylase (NCBI ptt file), at 2,660,149 to 2,660,640
def-3
SO2532: SO2532 - methylated-DNA--protein-cysteine methyltransferase, putative (NCBI ptt file), at 2,660,934 to 2,661,293
SO2532
SO2533: SO2533 - conserved hypothetical protein (NCBI ptt file), at 2,661,423 to 2,662,223
SO2533
SO2535: SO2535 - hypothetical amidotransferase (NCBI ptt file), at 2,662,363 to 2,663,142
SO2535
Position (kb)
2661
2662
2663 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2660.477 kb on + strand, within def-3 at 2660.477 kb on + strand, within def-3 at 2660.532 kb on - strand, within def-3 at 2660.532 kb on - strand, within def-3 at 2660.581 kb on + strand, within def-3 at 2660.680 kb on + strand at 2660.817 kb on - strand at 2660.933 kb on - strand at 2660.968 kb on + strand at 2661.004 kb on - strand, within SO2532 at 2661.123 kb on - strand, within SO2532 at 2661.317 kb on + strand at 2661.381 kb on - strand at 2661.406 kb on + strand at 2661.406 kb on + strand at 2661.414 kb on - strand at 2661.451 kb on + strand at 2661.451 kb on + strand at 2661.654 kb on - strand, within SO2533 at 2661.683 kb on + strand, within SO2533 at 2661.691 kb on - strand, within SO2533 at 2661.696 kb on + strand, within SO2533 at 2661.766 kb on - strand, within SO2533 at 2661.766 kb on - strand, within SO2533 at 2661.982 kb on - strand, within SO2533 at 2662.085 kb on + strand, within SO2533 at 2662.097 kb on + strand, within SO2533 at 2662.130 kb on + strand, within SO2533 at 2662.253 kb on - strand at 2662.545 kb on - strand, within SO2535 at 2662.569 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.634 kb on - strand, within SO2535 at 2662.634 kb on - strand, within SO2535 at 2662.634 kb on - strand, within SO2535 at 2662.738 kb on - strand, within SO2535 at 2662.761 kb on + strand, within SO2535 at 2662.818 kb on - strand, within SO2535 at 2662.949 kb on - strand, within SO2535 at 2662.954 kb on - strand, within SO2535 at 2663.008 kb on + strand, within SO2535 at 2663.016 kb on - strand, within SO2535 at 2663.025 kb on + strand, within SO2535 at 2663.073 kb on + strand at 2663.073 kb on + strand at 2663.081 kb on - strand at 2663.081 kb on - strand at 2663.082 kb on - strand at 2663.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,660,477 + def-3 SO2530 0.67 +0.1 2,660,477 + def-3 SO2530 0.67 -0.0 2,660,532 - def-3 SO2530 0.78 -0.1 2,660,532 - def-3 SO2530 0.78 +0.0 2,660,581 + def-3 SO2530 0.88 +0.7 2,660,680 + +1.2 2,660,817 - -2.4 2,660,933 - -0.7 2,660,968 + +0.5 2,661,004 - SO2532 0.19 -0.3 2,661,123 - SO2532 0.53 -1.4 2,661,317 + -1.5 2,661,381 - -1.1 2,661,406 + -0.9 2,661,406 + -1.8 2,661,414 - -1.1 2,661,451 + -1.7 2,661,451 + -0.8 2,661,654 - SO2533 0.29 -0.9 2,661,683 + SO2533 0.32 -1.5 2,661,691 - SO2533 0.33 -2.8 2,661,696 + SO2533 0.34 -1.0 2,661,766 - SO2533 0.43 -1.2 2,661,766 - SO2533 0.43 -1.1 2,661,982 - SO2533 0.70 -0.1 2,662,085 + SO2533 0.83 -0.8 2,662,097 + SO2533 0.84 -4.1 2,662,130 + SO2533 0.88 -0.4 2,662,253 - +0.5 2,662,545 - SO2535 0.23 -0.6 2,662,569 + SO2535 0.26 -0.4 2,662,626 + SO2535 0.34 -2.4 2,662,626 + SO2535 0.34 -0.1 2,662,626 + SO2535 0.34 -0.0 2,662,626 + SO2535 0.34 -1.1 2,662,634 - SO2535 0.35 -2.4 2,662,634 - SO2535 0.35 -1.2 2,662,634 - SO2535 0.35 -0.1 2,662,738 - SO2535 0.48 -1.0 2,662,761 + SO2535 0.51 -2.0 2,662,818 - SO2535 0.58 -1.8 2,662,949 - SO2535 0.75 -1.7 2,662,954 - SO2535 0.76 -1.2 2,663,008 + SO2535 0.83 +0.2 2,663,016 - SO2535 0.84 -1.1 2,663,025 + SO2535 0.85 +1.8 2,663,073 + -3.1 2,663,073 + -0.2 2,663,081 - -2.5 2,663,081 - -0.8 2,663,082 - -0.3 2,663,105 + +0.9
Or see this region's nucleotide sequence