Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvI and SO2280 are separated by 450 nucleotides SO2280 and SO2281 are separated by 473 nucleotides
SO2279: ilvI - acetolactate synthase III, large subunit (NCBI ptt file), at 2,389,858 to 2,391,576
ilvI
SO2280: SO2280 - bicyclomycin resistance protein (NCBI ptt file), at 2,392,027 to 2,393,250
SO2280
SO2281: SO2281 - alkaline phosphatase (NCBI ptt file), at 2,393,724 to 2,395,598
SO2281
Position (kb)
2392
2393
2394 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2391.343 kb on + strand, within ilvI at 2391.348 kb on + strand, within ilvI at 2391.356 kb on - strand, within ilvI at 2391.356 kb on - strand, within ilvI at 2391.356 kb on - strand, within ilvI at 2391.531 kb on + strand at 2392.013 kb on + strand at 2392.122 kb on - strand at 2392.122 kb on - strand at 2392.138 kb on - strand at 2392.279 kb on - strand, within SO2280 at 2392.336 kb on + strand, within SO2280 at 2392.336 kb on + strand, within SO2280 at 2392.483 kb on - strand, within SO2280 at 2392.600 kb on - strand, within SO2280 at 2392.658 kb on - strand, within SO2280 at 2392.693 kb on + strand, within SO2280 at 2392.971 kb on - strand, within SO2280 at 2392.995 kb on + strand, within SO2280 at 2393.007 kb on + strand, within SO2280 at 2393.193 kb on + strand at 2393.414 kb on + strand at 2393.427 kb on - strand at 2393.442 kb on + strand at 2393.450 kb on - strand at 2393.469 kb on - strand at 2393.647 kb on - strand at 2393.783 kb on + strand at 2393.853 kb on + strand at 2393.873 kb on + strand at 2393.881 kb on - strand at 2393.995 kb on - strand, within SO2281 at 2394.024 kb on - strand, within SO2281 at 2394.134 kb on + strand, within SO2281 at 2394.174 kb on + strand, within SO2281
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,391,343 + ilvI SO2279 0.86 -4.2 2,391,348 + ilvI SO2279 0.87 -2.8 2,391,356 - ilvI SO2279 0.87 -4.8 2,391,356 - ilvI SO2279 0.87 -3.0 2,391,356 - ilvI SO2279 0.87 -5.2 2,391,531 + -4.1 2,392,013 + -0.3 2,392,122 - -5.2 2,392,122 - -4.4 2,392,138 - -4.4 2,392,279 - SO2280 0.21 -2.1 2,392,336 + SO2280 0.25 -1.4 2,392,336 + SO2280 0.25 -3.7 2,392,483 - SO2280 0.37 -5.2 2,392,600 - SO2280 0.47 -5.0 2,392,658 - SO2280 0.52 -2.4 2,392,693 + SO2280 0.54 -4.7 2,392,971 - SO2280 0.77 -4.7 2,392,995 + SO2280 0.79 -2.4 2,393,007 + SO2280 0.80 -4.0 2,393,193 + -1.8 2,393,414 + -4.2 2,393,427 - -0.2 2,393,442 + -3.7 2,393,450 - -4.4 2,393,469 - -3.8 2,393,647 - +1.3 2,393,783 + +0.1 2,393,853 + -0.6 2,393,873 + -0.7 2,393,881 - -2.1 2,393,995 - SO2281 0.14 +1.2 2,394,024 - SO2281 0.16 +1.0 2,394,134 + SO2281 0.22 -1.2 2,394,174 + SO2281 0.24 -0.2
Or see this region's nucleotide sequence