Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1910 and SO1911 are separated by 388 nucleotides SO1911 and tesB are separated by 123 nucleotides tesB and SO1913 are separated by 7 nucleotides
SO1910: SO1910 - 1,4-dihydroxy-2-naphthoate octaprenyltransferase, putative (NCBI ptt file), at 2,008,349 to 2,009,224
SO1910
SO1911: SO1911 - oxidoreductase, short chain dehydrogenase/reductase family (NCBI ptt file), at 2,009,613 to 2,010,476
SO1911
SO1912: tesB - acyl-CoA thioesterase II (NCBI ptt file), at 2,010,600 to 2,011,466
tesB
SO1913: SO1913 - conserved hypothetical protein (NCBI ptt file), at 2,011,474 to 2,011,872
SO1913
Position (kb)
2009
2010
2011 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2008.707 kb on + strand, within SO1910 at 2008.722 kb on + strand, within SO1910 at 2008.734 kb on + strand, within SO1910 at 2008.758 kb on - strand, within SO1910 at 2008.800 kb on + strand, within SO1910 at 2008.808 kb on - strand, within SO1910 at 2008.956 kb on + strand, within SO1910 at 2009.146 kb on - strand at 2009.183 kb on + strand at 2009.190 kb on + strand at 2009.228 kb on + strand at 2009.253 kb on + strand at 2009.261 kb on - strand at 2009.303 kb on + strand at 2009.386 kb on - strand at 2009.589 kb on + strand at 2009.589 kb on + strand at 2009.589 kb on + strand at 2009.589 kb on + strand at 2009.589 kb on + strand at 2009.589 kb on + strand at 2009.597 kb on - strand at 2009.597 kb on - strand at 2009.597 kb on - strand at 2009.597 kb on - strand at 2009.597 kb on - strand at 2009.597 kb on - strand at 2009.597 kb on - strand at 2009.599 kb on - strand at 2009.629 kb on + strand at 2009.706 kb on + strand, within SO1911 at 2009.741 kb on - strand, within SO1911 at 2009.871 kb on - strand, within SO1911 at 2009.872 kb on - strand, within SO1911 at 2009.896 kb on - strand, within SO1911 at 2009.913 kb on - strand, within SO1911 at 2009.976 kb on + strand, within SO1911 at 2010.031 kb on - strand, within SO1911 at 2010.080 kb on + strand, within SO1911 at 2010.080 kb on + strand, within SO1911 at 2010.088 kb on - strand, within SO1911 at 2010.088 kb on - strand, within SO1911 at 2010.273 kb on + strand, within SO1911 at 2010.283 kb on + strand, within SO1911 at 2010.362 kb on + strand, within SO1911 at 2010.433 kb on + strand at 2010.538 kb on + strand at 2010.538 kb on + strand at 2010.546 kb on - strand at 2010.548 kb on + strand at 2010.557 kb on + strand at 2010.565 kb on - strand at 2010.577 kb on - strand at 2010.615 kb on + strand at 2010.632 kb on + strand at 2010.713 kb on + strand, within tesB at 2010.721 kb on - strand, within tesB at 2010.735 kb on - strand, within tesB at 2010.762 kb on + strand, within tesB at 2010.978 kb on + strand, within tesB at 2010.984 kb on - strand, within tesB at 2011.085 kb on + strand, within tesB at 2011.151 kb on + strand, within tesB at 2011.164 kb on - strand, within tesB at 2011.340 kb on - strand, within tesB at 2011.351 kb on + strand, within tesB at 2011.382 kb on - strand at 2011.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,008,707 + SO1910 0.41 +2.5 2,008,722 + SO1910 0.43 -0.0 2,008,734 + SO1910 0.44 -1.3 2,008,758 - SO1910 0.47 -0.7 2,008,800 + SO1910 0.51 -0.5 2,008,808 - SO1910 0.52 -1.3 2,008,956 + SO1910 0.69 -0.2 2,009,146 - -0.9 2,009,183 + -0.5 2,009,190 + -0.4 2,009,228 + -0.3 2,009,253 + -0.4 2,009,261 - -2.5 2,009,303 + +0.9 2,009,386 - -0.0 2,009,589 + -0.0 2,009,589 + -0.3 2,009,589 + +0.1 2,009,589 + +0.6 2,009,589 + -0.5 2,009,589 + +0.4 2,009,597 - +0.5 2,009,597 - +0.7 2,009,597 - -0.9 2,009,597 - -0.9 2,009,597 - -2.1 2,009,597 - -0.1 2,009,597 - -1.6 2,009,599 - -1.7 2,009,629 + -0.6 2,009,706 + SO1911 0.11 -1.0 2,009,741 - SO1911 0.15 -1.7 2,009,871 - SO1911 0.30 +0.3 2,009,872 - SO1911 0.30 -0.0 2,009,896 - SO1911 0.33 -0.3 2,009,913 - SO1911 0.35 -0.9 2,009,976 + SO1911 0.42 -0.6 2,010,031 - SO1911 0.48 +0.8 2,010,080 + SO1911 0.54 -0.2 2,010,080 + SO1911 0.54 +0.8 2,010,088 - SO1911 0.55 -1.3 2,010,088 - SO1911 0.55 -1.2 2,010,273 + SO1911 0.76 -1.3 2,010,283 + SO1911 0.78 +0.3 2,010,362 + SO1911 0.87 +0.1 2,010,433 + -2.0 2,010,538 + -0.2 2,010,538 + -0.1 2,010,546 - -0.3 2,010,548 + -0.7 2,010,557 + -1.9 2,010,565 - -0.3 2,010,577 - -0.5 2,010,615 + -0.1 2,010,632 + -0.5 2,010,713 + tesB SO1912 0.13 -0.4 2,010,721 - tesB SO1912 0.14 -0.2 2,010,735 - tesB SO1912 0.16 -0.3 2,010,762 + tesB SO1912 0.19 +0.0 2,010,978 + tesB SO1912 0.44 +0.3 2,010,984 - tesB SO1912 0.44 -0.2 2,011,085 + tesB SO1912 0.56 -1.4 2,011,151 + tesB SO1912 0.64 -1.4 2,011,164 - tesB SO1912 0.65 +1.6 2,011,340 - tesB SO1912 0.85 +1.5 2,011,351 + tesB SO1912 0.87 -0.1 2,011,382 - +0.6 2,011,445 - +0.6
Or see this region's nucleotide sequence