Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1814 and SO1815 are separated by 21 nucleotides SO1815 and SO1816 are separated by 5 nucleotides SO1816 and SO1817 are separated by 9 nucleotides SO1817 and SO1818 overlap by 10 nucleotides
SO1814: SO1814 - hypothetical protein (NCBI ptt file), at 1,904,444 to 1,904,722
SO1814
SO1815: SO1815 - histone deacetylase/AcuC/AphA family protein (NCBI ptt file), at 1,904,744 to 1,905,658
SO1815
SO1816: SO1816 - conserved hypothetical protein (NCBI ptt file), at 1,905,664 to 1,906,344
SO1816
SO1817: SO1817 - primosomal replication protein n'', putative (NCBI ptt file), at 1,906,354 to 1,907,022
SO1817
SO1818: SO1818 - conserved hypothetical protein (NCBI ptt file), at 1,907,013 to 1,907,786
SO1818
Position (kb)
1905
1906
1907 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1904.892 kb on - strand, within SO1815 at 1904.897 kb on - strand, within SO1815 at 1904.899 kb on + strand, within SO1815 at 1904.907 kb on - strand, within SO1815 at 1904.926 kb on - strand, within SO1815 at 1904.930 kb on + strand, within SO1815 at 1904.972 kb on - strand, within SO1815 at 1904.973 kb on - strand, within SO1815 at 1905.014 kb on + strand, within SO1815 at 1905.027 kb on - strand, within SO1815 at 1905.165 kb on - strand, within SO1815 at 1905.233 kb on - strand, within SO1815 at 1905.276 kb on + strand, within SO1815 at 1905.320 kb on - strand, within SO1815 at 1905.325 kb on - strand, within SO1815 at 1905.341 kb on + strand, within SO1815 at 1905.430 kb on + strand, within SO1815 at 1905.468 kb on + strand, within SO1815 at 1905.476 kb on - strand, within SO1815 at 1905.530 kb on - strand, within SO1815 at 1905.568 kb on + strand at 1905.604 kb on + strand at 1905.750 kb on + strand, within SO1816 at 1905.750 kb on + strand, within SO1816 at 1905.765 kb on + strand, within SO1816 at 1905.829 kb on - strand, within SO1816 at 1905.907 kb on - strand, within SO1816 at 1905.975 kb on - strand, within SO1816 at 1905.982 kb on + strand, within SO1816 at 1905.982 kb on + strand, within SO1816 at 1905.990 kb on - strand, within SO1816 at 1905.990 kb on - strand, within SO1816 at 1905.990 kb on - strand, within SO1816 at 1906.085 kb on - strand, within SO1816 at 1906.278 kb on - strand at 1906.374 kb on - strand at 1906.557 kb on - strand, within SO1817 at 1906.932 kb on - strand, within SO1817 at 1907.001 kb on + strand at 1907.076 kb on - strand at 1907.164 kb on - strand, within SO1818 at 1907.185 kb on - strand, within SO1818 at 1907.230 kb on + strand, within SO1818 at 1907.238 kb on - strand, within SO1818
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,904,892 - SO1815 0.16 -1.9 1,904,897 - SO1815 0.17 -2.5 1,904,899 + SO1815 0.17 -1.9 1,904,907 - SO1815 0.18 -1.9 1,904,926 - SO1815 0.20 -2.4 1,904,930 + SO1815 0.20 -0.3 1,904,972 - SO1815 0.25 -0.5 1,904,973 - SO1815 0.25 -3.4 1,905,014 + SO1815 0.30 -0.7 1,905,027 - SO1815 0.31 -1.5 1,905,165 - SO1815 0.46 -0.9 1,905,233 - SO1815 0.53 -2.2 1,905,276 + SO1815 0.58 -2.3 1,905,320 - SO1815 0.63 -2.3 1,905,325 - SO1815 0.63 -2.7 1,905,341 + SO1815 0.65 -0.7 1,905,430 + SO1815 0.75 -3.7 1,905,468 + SO1815 0.79 -1.5 1,905,476 - SO1815 0.80 -1.7 1,905,530 - SO1815 0.86 -0.9 1,905,568 + -2.6 1,905,604 + -1.9 1,905,750 + SO1816 0.13 +1.0 1,905,750 + SO1816 0.13 -0.3 1,905,765 + SO1816 0.15 -2.0 1,905,829 - SO1816 0.24 -0.7 1,905,907 - SO1816 0.36 -1.2 1,905,975 - SO1816 0.46 +0.4 1,905,982 + SO1816 0.47 +0.1 1,905,982 + SO1816 0.47 -0.2 1,905,990 - SO1816 0.48 -3.8 1,905,990 - SO1816 0.48 -0.1 1,905,990 - SO1816 0.48 -1.6 1,906,085 - SO1816 0.62 -1.3 1,906,278 - -0.3 1,906,374 - +1.2 1,906,557 - SO1817 0.30 -0.2 1,906,932 - SO1817 0.86 +0.4 1,907,001 + +2.1 1,907,076 - +3.0 1,907,164 - SO1818 0.20 +2.8 1,907,185 - SO1818 0.22 +3.3 1,907,230 + SO1818 0.28 +3.4 1,907,238 - SO1818 0.29 +3.2
Or see this region's nucleotide sequence