Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1548 and xni are separated by 107 nucleotides xni and SO1550 are separated by 89 nucleotides SO1550 and SO1551 are separated by 56 nucleotides
SO1548: SO1548 - hypothetical protein (NCBI ptt file), at 1,623,349 to 1,623,777
SO1548
SO1549: xni - exodeoxyribonuclease IX (NCBI ptt file), at 1,623,885 to 1,624,595
xni
SO1550: SO1550 - conserved hypothetical protein (NCBI ptt file), at 1,624,685 to 1,626,040
SO1550
SO1551: SO1551 - GGDEF domain protein (NCBI ptt file), at 1,626,097 to 1,627,653
SO1551
Position (kb)
1624
1625
1626
1627 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1623.686 kb on + strand, within SO1548 at 1624.282 kb on - strand, within xni at 1624.312 kb on + strand, within xni at 1624.312 kb on + strand, within xni at 1624.432 kb on + strand, within xni at 1624.561 kb on - strand at 1624.578 kb on - strand at 1624.588 kb on - strand at 1624.612 kb on - strand at 1624.614 kb on - strand at 1624.727 kb on - strand at 1624.737 kb on - strand at 1624.797 kb on + strand at 1624.797 kb on + strand at 1624.799 kb on - strand at 1624.805 kb on - strand at 1624.805 kb on - strand at 1624.822 kb on - strand, within SO1550 at 1624.888 kb on + strand, within SO1550 at 1624.896 kb on - strand, within SO1550 at 1624.945 kb on + strand, within SO1550 at 1625.055 kb on - strand, within SO1550 at 1625.127 kb on - strand, within SO1550 at 1625.303 kb on + strand, within SO1550 at 1625.418 kb on - strand, within SO1550 at 1625.418 kb on - strand, within SO1550 at 1625.418 kb on - strand, within SO1550 at 1625.418 kb on - strand, within SO1550 at 1625.458 kb on - strand, within SO1550 at 1625.577 kb on - strand, within SO1550 at 1625.695 kb on + strand, within SO1550 at 1625.695 kb on + strand, within SO1550 at 1625.703 kb on - strand, within SO1550 at 1625.703 kb on - strand, within SO1550 at 1625.703 kb on - strand, within SO1550 at 1625.703 kb on - strand, within SO1550 at 1625.703 kb on - strand, within SO1550 at 1625.741 kb on - strand, within SO1550 at 1625.792 kb on + strand, within SO1550 at 1625.800 kb on - strand, within SO1550 at 1625.850 kb on - strand, within SO1550 at 1625.900 kb on + strand, within SO1550 at 1626.036 kb on - strand at 1626.152 kb on + strand at 1626.209 kb on + strand at 1626.413 kb on + strand, within SO1551 at 1626.420 kb on - strand, within SO1551 at 1626.428 kb on - strand, within SO1551 at 1626.433 kb on - strand, within SO1551 at 1626.545 kb on - strand, within SO1551 at 1626.572 kb on - strand, within SO1551 at 1626.620 kb on - strand, within SO1551 at 1626.674 kb on - strand, within SO1551 at 1626.707 kb on - strand, within SO1551 at 1626.707 kb on - strand, within SO1551 at 1626.707 kb on - strand, within SO1551 at 1626.726 kb on - strand, within SO1551 at 1626.726 kb on - strand, within SO1551 at 1626.745 kb on - strand, within SO1551 at 1626.747 kb on + strand, within SO1551 at 1626.755 kb on - strand, within SO1551 at 1626.867 kb on + strand, within SO1551 at 1626.867 kb on + strand, within SO1551 at 1626.867 kb on + strand, within SO1551 at 1626.875 kb on - strand, within SO1551 at 1626.886 kb on + strand, within SO1551
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,623,686 + SO1548 0.79 -0.8 1,624,282 - xni SO1549 0.56 -0.8 1,624,312 + xni SO1549 0.60 +0.0 1,624,312 + xni SO1549 0.60 +1.7 1,624,432 + xni SO1549 0.77 -0.8 1,624,561 - -0.3 1,624,578 - -0.3 1,624,588 - -0.6 1,624,612 - -0.1 1,624,614 - +0.2 1,624,727 - +0.5 1,624,737 - -2.6 1,624,797 + -1.8 1,624,797 + -3.2 1,624,799 - -1.5 1,624,805 - +0.2 1,624,805 - -1.3 1,624,822 - SO1550 0.10 -1.2 1,624,888 + SO1550 0.15 -2.8 1,624,896 - SO1550 0.16 -1.7 1,624,945 + SO1550 0.19 -1.0 1,625,055 - SO1550 0.27 -1.5 1,625,127 - SO1550 0.33 -2.6 1,625,303 + SO1550 0.46 -0.5 1,625,418 - SO1550 0.54 -0.3 1,625,418 - SO1550 0.54 -2.0 1,625,418 - SO1550 0.54 -0.9 1,625,418 - SO1550 0.54 -4.1 1,625,458 - SO1550 0.57 -2.0 1,625,577 - SO1550 0.66 -2.0 1,625,695 + SO1550 0.74 -2.3 1,625,695 + SO1550 0.74 -0.7 1,625,703 - SO1550 0.75 -1.5 1,625,703 - SO1550 0.75 -0.4 1,625,703 - SO1550 0.75 -1.5 1,625,703 - SO1550 0.75 -2.5 1,625,703 - SO1550 0.75 -3.5 1,625,741 - SO1550 0.78 -3.9 1,625,792 + SO1550 0.82 +0.0 1,625,800 - SO1550 0.82 -3.3 1,625,850 - SO1550 0.86 -2.6 1,625,900 + SO1550 0.90 -2.3 1,626,036 - -2.3 1,626,152 + -1.3 1,626,209 + -0.8 1,626,413 + SO1551 0.20 +1.7 1,626,420 - SO1551 0.21 +1.3 1,626,428 - SO1551 0.21 +1.0 1,626,433 - SO1551 0.22 +1.6 1,626,545 - SO1551 0.29 +1.6 1,626,572 - SO1551 0.31 -0.1 1,626,620 - SO1551 0.34 +1.1 1,626,674 - SO1551 0.37 -0.3 1,626,707 - SO1551 0.39 +0.1 1,626,707 - SO1551 0.39 +1.1 1,626,707 - SO1551 0.39 +1.5 1,626,726 - SO1551 0.40 +0.1 1,626,726 - SO1551 0.40 +0.1 1,626,745 - SO1551 0.42 +0.3 1,626,747 + SO1551 0.42 -1.5 1,626,755 - SO1551 0.42 +0.6 1,626,867 + SO1551 0.49 +0.9 1,626,867 + SO1551 0.49 +0.3 1,626,867 + SO1551 0.49 +0.4 1,626,875 - SO1551 0.50 +0.9 1,626,886 + SO1551 0.51 +0.1
Or see this region's nucleotide sequence