Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0748 and SO0749 are separated by 107 nucleotides SO0749 and SO0750 are separated by 11 nucleotides SO0750 and SO0752 are separated by 415 nucleotides SO0752 and SO0753 are separated by 56 nucleotides
SO0748: SO0748 - hypothetical protein (NCBI ptt file), at 765,469 to 766,146
SO0748
SO0749: SO0749 - conserved hypothetical protein (NCBI ptt file), at 766,254 to 766,847
SO0749
SO0750: SO0750 - glutamate synthase, putative (NCBI ptt file), at 766,859 to 768,349
SO0750
SO0752: SO0752 - hypothetical protein (NCBI ptt file), at 768,765 to 768,971
SO0752
SO0753: SO0753 - hypothetical protein (NCBI ptt file), at 769,028 to 769,285
SO0753
Position (kb) 766
767
768
769 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 766.025 kb on + strand, within SO0748 at 766.025 kb on + strand, within SO0748 at 766.025 kb on + strand, within SO0748 at 766.030 kb on + strand, within SO0748 at 766.035 kb on + strand, within SO0748 at 766.038 kb on - strand, within SO0748 at 766.152 kb on - strand at 766.216 kb on - strand at 766.363 kb on + strand, within SO0749 at 766.363 kb on + strand, within SO0749 at 766.363 kb on + strand, within SO0749 at 766.363 kb on + strand, within SO0749 at 766.363 kb on + strand, within SO0749 at 766.363 kb on + strand, within SO0749 at 766.371 kb on - strand, within SO0749 at 766.371 kb on - strand, within SO0749 at 766.371 kb on - strand, within SO0749 at 766.456 kb on + strand, within SO0749 at 766.496 kb on - strand, within SO0749 at 766.560 kb on + strand, within SO0749 at 766.700 kb on + strand, within SO0749 at 766.708 kb on - strand, within SO0749 at 766.708 kb on - strand, within SO0749 at 766.839 kb on + strand at 766.845 kb on - strand at 766.946 kb on + strand at 767.098 kb on - strand, within SO0750 at 767.120 kb on - strand, within SO0750 at 767.120 kb on - strand, within SO0750 at 767.172 kb on + strand, within SO0750 at 767.180 kb on - strand, within SO0750 at 767.351 kb on + strand, within SO0750 at 767.357 kb on - strand, within SO0750 at 767.359 kb on - strand, within SO0750 at 767.359 kb on - strand, within SO0750 at 767.372 kb on - strand, within SO0750 at 767.394 kb on - strand, within SO0750 at 767.406 kb on + strand, within SO0750 at 767.415 kb on - strand, within SO0750 at 767.430 kb on - strand, within SO0750 at 767.430 kb on - strand, within SO0750 at 767.462 kb on + strand, within SO0750 at 767.462 kb on + strand, within SO0750 at 767.601 kb on + strand, within SO0750 at 767.609 kb on - strand, within SO0750 at 767.637 kb on + strand, within SO0750 at 767.654 kb on + strand, within SO0750 at 767.831 kb on - strand, within SO0750 at 767.887 kb on - strand, within SO0750 at 767.970 kb on + strand, within SO0750 at 767.986 kb on - strand, within SO0750 at 768.018 kb on + strand, within SO0750 at 768.023 kb on + strand, within SO0750 at 768.028 kb on + strand, within SO0750 at 768.036 kb on - strand, within SO0750 at 768.085 kb on + strand, within SO0750 at 768.087 kb on + strand, within SO0750 at 768.159 kb on + strand, within SO0750 at 768.167 kb on - strand, within SO0750 at 768.167 kb on - strand, within SO0750 at 768.212 kb on + strand at 768.219 kb on + strand at 768.219 kb on + strand at 768.227 kb on - strand at 768.246 kb on - strand at 768.246 kb on - strand at 768.270 kb on + strand at 768.325 kb on + strand at 768.690 kb on - strand at 768.797 kb on + strand, within SO0752 at 768.876 kb on + strand, within SO0752 at 769.088 kb on + strand, within SO0753 at 769.135 kb on + strand, within SO0753 at 769.143 kb on - strand, within SO0753 at 769.146 kb on + strand, within SO0753 at 769.146 kb on + strand, within SO0753 at 769.148 kb on + strand, within SO0753 at 769.154 kb on - strand, within SO0753 at 769.216 kb on + strand, within SO0753 at 769.230 kb on + strand, within SO0753 at 769.258 kb on - strand, within SO0753
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 766,025 + SO0748 0.82 +0.0 766,025 + SO0748 0.82 +1.2 766,025 + SO0748 0.82 -0.1 766,030 + SO0748 0.83 -0.5 766,035 + SO0748 0.83 +0.3 766,038 - SO0748 0.84 -1.0 766,152 - -0.8 766,216 - +0.2 766,363 + SO0749 0.18 -3.7 766,363 + SO0749 0.18 +0.4 766,363 + SO0749 0.18 -0.2 766,363 + SO0749 0.18 +1.0 766,363 + SO0749 0.18 -1.3 766,363 + SO0749 0.18 +0.3 766,371 - SO0749 0.20 +0.5 766,371 - SO0749 0.20 -1.1 766,371 - SO0749 0.20 +1.0 766,456 + SO0749 0.34 +0.5 766,496 - SO0749 0.41 -0.7 766,560 + SO0749 0.52 -2.0 766,700 + SO0749 0.75 +0.5 766,708 - SO0749 0.76 -0.3 766,708 - SO0749 0.76 +2.2 766,839 + +1.9 766,845 - +0.9 766,946 + -2.1 767,098 - SO0750 0.16 -0.1 767,120 - SO0750 0.18 -0.5 767,120 - SO0750 0.18 -0.3 767,172 + SO0750 0.21 +0.5 767,180 - SO0750 0.22 -0.3 767,351 + SO0750 0.33 -1.4 767,357 - SO0750 0.33 +0.5 767,359 - SO0750 0.34 +0.5 767,359 - SO0750 0.34 +0.4 767,372 - SO0750 0.34 -0.2 767,394 - SO0750 0.36 +1.6 767,406 + SO0750 0.37 -0.0 767,415 - SO0750 0.37 +0.1 767,430 - SO0750 0.38 -0.3 767,430 - SO0750 0.38 -1.0 767,462 + SO0750 0.40 +2.4 767,462 + SO0750 0.40 +0.8 767,601 + SO0750 0.50 -0.5 767,609 - SO0750 0.50 +0.4 767,637 + SO0750 0.52 +0.0 767,654 + SO0750 0.53 +0.2 767,831 - SO0750 0.65 -0.4 767,887 - SO0750 0.69 +1.6 767,970 + SO0750 0.75 +0.3 767,986 - SO0750 0.76 +0.5 768,018 + SO0750 0.78 +2.0 768,023 + SO0750 0.78 +0.8 768,028 + SO0750 0.78 -1.2 768,036 - SO0750 0.79 +0.3 768,085 + SO0750 0.82 +0.5 768,087 + SO0750 0.82 +1.3 768,159 + SO0750 0.87 +3.9 768,167 - SO0750 0.88 +0.0 768,167 - SO0750 0.88 +1.6 768,212 + -0.7 768,219 + +0.5 768,219 + -0.7 768,227 - -0.3 768,246 - -0.9 768,246 - +2.4 768,270 + -1.0 768,325 + +0.2 768,690 - +0.1 768,797 + SO0752 0.15 -1.0 768,876 + SO0752 0.54 -0.1 769,088 + SO0753 0.23 +1.3 769,135 + SO0753 0.41 -0.3 769,143 - SO0753 0.45 +0.5 769,146 + SO0753 0.46 +1.1 769,146 + SO0753 0.46 -0.2 769,148 + SO0753 0.47 -0.7 769,154 - SO0753 0.49 +0.5 769,216 + SO0753 0.73 +1.4 769,230 + SO0753 0.78 -0.5 769,258 - SO0753 0.89 +2.0
Or see this region's nucleotide sequence