Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pilD and SO0415 are separated by 165 nucleotides SO0415 and SO0416 are separated by 251 nucleotides
SO0414: pilD - type 4 prepilin-like proteins leader peptide processing enzyme (NCBI ptt file), at 428,993 to 429,919
pilD
SO0415: SO0415 - type IV pilus biogenesis protein PilC (NCBI ptt file), at 430,085 to 431,356
SO0415
SO0416: SO0416 - type IV pilus biogenesis protein PilB (NCBI ptt file), at 431,608 to 433,317
SO0416
Position (kb) 430
431
432 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 429.105 kb on - strand, within pilD at 429.337 kb on + strand, within pilD at 429.355 kb on + strand, within pilD at 429.363 kb on - strand, within pilD at 429.511 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.598 kb on - strand, within pilD at 429.688 kb on - strand, within pilD at 429.993 kb on - strand at 430.106 kb on - strand at 430.126 kb on - strand at 430.143 kb on - strand at 430.217 kb on - strand, within SO0415 at 430.236 kb on + strand, within SO0415 at 430.236 kb on + strand, within SO0415 at 430.242 kb on + strand, within SO0415 at 430.268 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.541 kb on + strand, within SO0415 at 430.542 kb on + strand, within SO0415 at 430.542 kb on + strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.552 kb on + strand, within SO0415 at 430.560 kb on - strand, within SO0415 at 430.562 kb on - strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.788 kb on + strand, within SO0415 at 430.841 kb on + strand, within SO0415 at 430.867 kb on + strand, within SO0415 at 430.896 kb on - strand, within SO0415 at 430.945 kb on + strand, within SO0415 at 431.184 kb on - strand, within SO0415 at 431.184 kb on - strand, within SO0415 at 431.261 kb on + strand at 431.431 kb on - strand at 431.570 kb on + strand at 431.570 kb on + strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.703 kb on + strand at 431.703 kb on - strand at 431.811 kb on + strand, within SO0416 at 431.837 kb on + strand, within SO0416 at 431.837 kb on + strand, within SO0416 at 431.837 kb on + strand, within SO0416 at 431.956 kb on - strand, within SO0416 at 432.028 kb on + strand, within SO0416 at 432.030 kb on - strand, within SO0416 at 432.036 kb on - strand, within SO0416 at 432.166 kb on + strand, within SO0416 at 432.166 kb on + strand, within SO0416 at 432.230 kb on + strand, within SO0416 at 432.230 kb on + strand, within SO0416 at 432.230 kb on + strand, within SO0416 at 432.238 kb on - strand, within SO0416 at 432.239 kb on - strand, within SO0416 at 432.289 kb on + strand, within SO0416 at 432.299 kb on + strand, within SO0416 at 432.299 kb on + strand, within SO0416 at 432.322 kb on - strand, within SO0416 at 432.334 kb on - strand, within SO0416 at 432.334 kb on - strand, within SO0416
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 429,105 - pilD SO0414 0.12 +1.9 429,337 + pilD SO0414 0.37 +3.4 429,355 + pilD SO0414 0.39 +3.5 429,363 - pilD SO0414 0.40 +0.2 429,511 - pilD SO0414 0.56 +1.4 429,588 - pilD SO0414 0.64 +1.0 429,588 - pilD SO0414 0.64 +1.2 429,598 - pilD SO0414 0.65 -0.2 429,688 - pilD SO0414 0.75 +3.6 429,993 - -0.2 430,106 - -1.1 430,126 - +0.4 430,143 - -1.4 430,217 - SO0415 0.10 +0.6 430,236 + SO0415 0.12 +2.0 430,236 + SO0415 0.12 +1.7 430,242 + SO0415 0.12 -2.1 430,268 + SO0415 0.14 +0.5 430,318 + SO0415 0.18 +2.1 430,318 + SO0415 0.18 -0.4 430,318 + SO0415 0.18 +2.4 430,326 - SO0415 0.19 -0.3 430,326 - SO0415 0.19 +0.3 430,326 - SO0415 0.19 -0.9 430,541 + SO0415 0.36 +0.8 430,542 + SO0415 0.36 -1.3 430,542 + SO0415 0.36 +0.8 430,550 - SO0415 0.37 +0.5 430,550 - SO0415 0.37 -1.1 430,550 - SO0415 0.37 +0.7 430,552 + SO0415 0.37 +0.4 430,560 - SO0415 0.37 -0.4 430,562 - SO0415 0.38 -1.6 430,745 + SO0415 0.52 +2.7 430,745 + SO0415 0.52 +1.4 430,788 + SO0415 0.55 +0.6 430,841 + SO0415 0.59 +0.1 430,867 + SO0415 0.61 -0.9 430,896 - SO0415 0.64 +0.3 430,945 + SO0415 0.68 +0.2 431,184 - SO0415 0.86 +0.5 431,184 - SO0415 0.86 -2.1 431,261 + +0.3 431,431 - +0.6 431,570 + +0.9 431,570 + -1.4 431,578 - +0.8 431,578 - -0.8 431,578 - +1.1 431,578 - +0.5 431,578 - -0.6 431,578 - -0.7 431,703 + +1.5 431,703 - +0.9 431,811 + SO0416 0.12 +0.1 431,837 + SO0416 0.13 -1.5 431,837 + SO0416 0.13 +0.3 431,837 + SO0416 0.13 +0.8 431,956 - SO0416 0.20 -1.4 432,028 + SO0416 0.25 -0.5 432,030 - SO0416 0.25 +0.2 432,036 - SO0416 0.25 -1.8 432,166 + SO0416 0.33 -2.4 432,166 + SO0416 0.33 -1.6 432,230 + SO0416 0.36 -0.4 432,230 + SO0416 0.36 +0.8 432,230 + SO0416 0.36 +1.1 432,238 - SO0416 0.37 -0.1 432,239 - SO0416 0.37 +1.4 432,289 + SO0416 0.40 -1.8 432,299 + SO0416 0.40 +1.5 432,299 + SO0416 0.40 -0.3 432,322 - SO0416 0.42 +0.4 432,334 - SO0416 0.42 +2.4 432,334 - SO0416 0.42 -0.8
Or see this region's nucleotide sequence