Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acnA and prpC are separated by 80 nucleotides prpC and prpB are separated by 234 nucleotides
SO0343: acnA - aconitate hydratase 1 (NCBI ptt file), at 347,544 to 350,147
acnA
SO0344: prpC - 2-methylcitrate synthase (EC 2.3.3.5) <small>(from data)</small>, at 350,228 to 351,355
prpC
SO0345: prpB - methylisocitrate lyase (NCBI ptt file), at 351,590 to 352,468
prpB
Position (kb) 350
351
352 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 349.291 kb on - strand, within acnA at 349.420 kb on - strand, within acnA at 349.460 kb on + strand, within acnA at 349.460 kb on + strand, within acnA at 349.534 kb on - strand, within acnA at 349.540 kb on + strand, within acnA at 349.815 kb on - strand, within acnA at 350.026 kb on + strand at 350.026 kb on + strand at 350.080 kb on - strand at 350.107 kb on + strand at 350.138 kb on - strand at 350.140 kb on - strand at 350.148 kb on - strand at 350.148 kb on - strand at 350.224 kb on - strand at 350.229 kb on - strand at 350.337 kb on + strand at 350.345 kb on - strand, within prpC at 350.364 kb on + strand, within prpC at 350.388 kb on + strand, within prpC at 350.398 kb on + strand, within prpC at 350.414 kb on + strand, within prpC at 350.551 kb on + strand, within prpC at 350.559 kb on - strand, within prpC at 350.559 kb on - strand, within prpC at 350.562 kb on + strand, within prpC at 350.615 kb on - strand, within prpC at 350.623 kb on + strand, within prpC at 350.695 kb on - strand, within prpC at 350.777 kb on + strand, within prpC at 350.792 kb on - strand, within prpC at 350.842 kb on + strand, within prpC at 350.842 kb on + strand, within prpC at 350.850 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.045 kb on - strand, within prpC at 351.115 kb on + strand, within prpC at 351.163 kb on + strand, within prpC at 351.199 kb on - strand, within prpC at 351.277 kb on - strand at 351.287 kb on + strand at 351.288 kb on - strand at 351.295 kb on - strand at 351.317 kb on + strand at 351.455 kb on + strand at 351.474 kb on - strand at 351.575 kb on - strand at 351.587 kb on - strand at 351.668 kb on + strand at 351.668 kb on + strand at 351.668 kb on + strand at 351.668 kb on + strand at 351.676 kb on - strand at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.713 kb on - strand, within prpB at 351.776 kb on - strand, within prpB at 351.805 kb on - strand, within prpB at 351.866 kb on + strand, within prpB at 351.869 kb on - strand, within prpB at 351.910 kb on - strand, within prpB at 351.978 kb on - strand, within prpB at 352.131 kb on + strand, within prpB at 352.343 kb on + strand, within prpB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 349,291 - acnA SO0343 0.67 -2.2 349,420 - acnA SO0343 0.72 -2.5 349,460 + acnA SO0343 0.74 -1.3 349,460 + acnA SO0343 0.74 -3.4 349,534 - acnA SO0343 0.76 -3.3 349,540 + acnA SO0343 0.77 -3.0 349,815 - acnA SO0343 0.87 -4.4 350,026 + -1.1 350,026 + -2.8 350,080 - -1.9 350,107 + -0.3 350,138 - -3.3 350,140 - -3.9 350,148 - -4.2 350,148 - -1.3 350,224 - -2.9 350,229 - -4.1 350,337 + -3.8 350,345 - prpC SO0344 0.10 -2.7 350,364 + prpC SO0344 0.12 -3.8 350,388 + prpC SO0344 0.14 -0.8 350,398 + prpC SO0344 0.15 -5.1 350,414 + prpC SO0344 0.16 -4.0 350,551 + prpC SO0344 0.29 -1.9 350,559 - prpC SO0344 0.29 -3.8 350,559 - prpC SO0344 0.29 -3.5 350,562 + prpC SO0344 0.30 -4.1 350,615 - prpC SO0344 0.34 -4.1 350,623 + prpC SO0344 0.35 -5.5 350,695 - prpC SO0344 0.41 -3.3 350,777 + prpC SO0344 0.49 -2.6 350,792 - prpC SO0344 0.50 -2.7 350,842 + prpC SO0344 0.54 -4.3 350,842 + prpC SO0344 0.54 -4.4 350,850 - prpC SO0344 0.55 -3.7 351,029 - prpC SO0344 0.71 -0.3 351,029 - prpC SO0344 0.71 -2.8 351,029 - prpC SO0344 0.71 -3.4 351,029 - prpC SO0344 0.71 -3.5 351,045 - prpC SO0344 0.72 -4.0 351,115 + prpC SO0344 0.79 -1.3 351,163 + prpC SO0344 0.83 -4.9 351,199 - prpC SO0344 0.86 -2.6 351,277 - -5.2 351,287 + -2.2 351,288 - -2.9 351,295 - -5.5 351,317 + -4.9 351,455 + -2.3 351,474 - -3.0 351,575 - -4.8 351,587 - -1.8 351,668 + -3.5 351,668 + -3.2 351,668 + -4.0 351,668 + -1.1 351,676 - -2.5 351,680 + prpB SO0345 0.10 -3.5 351,680 + prpB SO0345 0.10 -3.6 351,680 + prpB SO0345 0.10 -4.1 351,680 + prpB SO0345 0.10 -1.8 351,680 + prpB SO0345 0.10 -2.7 351,680 + prpB SO0345 0.10 -2.6 351,713 - prpB SO0345 0.14 -2.6 351,776 - prpB SO0345 0.21 -3.0 351,805 - prpB SO0345 0.24 -2.9 351,866 + prpB SO0345 0.31 -3.0 351,869 - prpB SO0345 0.32 -2.3 351,910 - prpB SO0345 0.36 -1.3 351,978 - prpB SO0345 0.44 -4.8 352,131 + prpB SO0345 0.62 -3.3 352,343 + prpB SO0345 0.86 -4.8
Or see this region's nucleotide sequence