Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0319 and SO0320 are separated by 31 nucleotides SO0320 and SO0321 are separated by 96 nucleotides SO0321 and SO0322 are separated by 99 nucleotides
SO0319: SO0319 - conserved domain protein (NCBI ptt file), at 325,226 to 326,563
SO0319
SO0320: SO0320 - conserved domain protein (NCBI ptt file), at 326,595 to 327,602
SO0320
SO0321: SO0321 - hypothetical protein (NCBI ptt file), at 327,699 to 328,301
SO0321
SO0322: SO0322 - hypothetical protein (NCBI ptt file), at 328,401 to 328,982
SO0322
Position (kb) 326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 325.646 kb on + strand, within SO0319 at 325.646 kb on + strand, within SO0319 at 325.659 kb on - strand, within SO0319 at 325.754 kb on - strand, within SO0319 at 325.829 kb on + strand, within SO0319 at 325.847 kb on - strand, within SO0319 at 325.904 kb on - strand, within SO0319 at 325.916 kb on + strand, within SO0319 at 325.981 kb on - strand, within SO0319 at 326.004 kb on - strand, within SO0319 at 326.027 kb on - strand, within SO0319 at 326.061 kb on - strand, within SO0319 at 326.131 kb on + strand, within SO0319 at 326.194 kb on + strand, within SO0319 at 326.202 kb on - strand, within SO0319 at 326.243 kb on - strand, within SO0319 at 326.298 kb on - strand, within SO0319 at 326.314 kb on + strand, within SO0319 at 326.331 kb on + strand, within SO0319 at 326.331 kb on + strand, within SO0319 at 326.334 kb on - strand, within SO0319 at 326.446 kb on - strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.473 kb on - strand at 326.473 kb on - strand at 326.533 kb on + strand at 326.540 kb on + strand at 326.541 kb on - strand at 326.645 kb on - strand at 326.647 kb on - strand at 326.823 kb on + strand, within SO0320 at 326.823 kb on + strand, within SO0320 at 326.846 kb on - strand, within SO0320 at 326.846 kb on - strand, within SO0320 at 326.848 kb on + strand, within SO0320 at 326.870 kb on + strand, within SO0320 at 326.883 kb on - strand, within SO0320 at 326.918 kb on + strand, within SO0320 at 326.935 kb on + strand, within SO0320 at 326.935 kb on + strand, within SO0320 at 327.042 kb on - strand, within SO0320 at 327.053 kb on + strand, within SO0320 at 327.079 kb on + strand, within SO0320 at 327.082 kb on - strand, within SO0320 at 327.087 kb on - strand, within SO0320 at 327.088 kb on + strand, within SO0320 at 327.106 kb on - strand, within SO0320 at 327.109 kb on - strand, within SO0320 at 327.109 kb on - strand, within SO0320 at 327.221 kb on + strand, within SO0320 at 327.221 kb on + strand, within SO0320 at 327.226 kb on + strand, within SO0320 at 327.226 kb on + strand, within SO0320 at 327.226 kb on + strand, within SO0320 at 327.229 kb on - strand, within SO0320 at 327.229 kb on - strand, within SO0320 at 327.229 kb on - strand, within SO0320 at 327.229 kb on - strand, within SO0320 at 327.231 kb on + strand, within SO0320 at 327.361 kb on + strand, within SO0320 at 327.431 kb on + strand, within SO0320 at 327.431 kb on + strand, within SO0320 at 327.431 kb on + strand, within SO0320 at 327.431 kb on + strand, within SO0320 at 327.439 kb on - strand, within SO0320 at 327.439 kb on - strand, within SO0320 at 327.439 kb on - strand, within SO0320 at 327.483 kb on - strand, within SO0320 at 327.621 kb on + strand at 327.629 kb on - strand at 327.834 kb on + strand, within SO0321 at 327.931 kb on - strand, within SO0321 at 327.957 kb on + strand, within SO0321 at 327.976 kb on - strand, within SO0321 at 328.157 kb on - strand, within SO0321 at 328.212 kb on + strand, within SO0321 at 328.268 kb on + strand at 328.308 kb on - strand at 328.474 kb on + strand, within SO0322 at 328.505 kb on - strand, within SO0322 at 328.530 kb on + strand, within SO0322 at 328.538 kb on - strand, within SO0322 at 328.538 kb on - strand, within SO0322 at 328.540 kb on + strand, within SO0322 at 328.598 kb on + strand, within SO0322
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 325,646 + SO0319 0.31 +1.8 325,646 + SO0319 0.31 +0.5 325,659 - SO0319 0.32 +1.3 325,754 - SO0319 0.39 +0.2 325,829 + SO0319 0.45 +0.8 325,847 - SO0319 0.46 -1.2 325,904 - SO0319 0.51 +0.2 325,916 + SO0319 0.52 -1.2 325,981 - SO0319 0.56 +0.5 326,004 - SO0319 0.58 -0.1 326,027 - SO0319 0.60 -0.4 326,061 - SO0319 0.62 -0.3 326,131 + SO0319 0.68 +0.4 326,194 + SO0319 0.72 +0.6 326,202 - SO0319 0.73 -2.1 326,243 - SO0319 0.76 +1.2 326,298 - SO0319 0.80 -0.3 326,314 + SO0319 0.81 -2.8 326,331 + SO0319 0.83 -1.3 326,331 + SO0319 0.83 -0.5 326,334 - SO0319 0.83 -0.3 326,446 - +0.1 326,465 + -1.1 326,465 + -0.1 326,465 + +0.7 326,473 - +0.6 326,473 - +2.1 326,533 + +1.8 326,540 + +1.7 326,541 - +0.4 326,645 - +2.2 326,647 - +0.5 326,823 + SO0320 0.23 +1.1 326,823 + SO0320 0.23 -0.1 326,846 - SO0320 0.25 -0.8 326,846 - SO0320 0.25 +1.5 326,848 + SO0320 0.25 +1.4 326,870 + SO0320 0.27 +0.2 326,883 - SO0320 0.29 +2.8 326,918 + SO0320 0.32 +1.3 326,935 + SO0320 0.34 -0.0 326,935 + SO0320 0.34 +0.5 327,042 - SO0320 0.44 -0.7 327,053 + SO0320 0.45 +0.9 327,079 + SO0320 0.48 +1.4 327,082 - SO0320 0.48 +1.6 327,087 - SO0320 0.49 +1.8 327,088 + SO0320 0.49 -0.0 327,106 - SO0320 0.51 +2.0 327,109 - SO0320 0.51 +1.8 327,109 - SO0320 0.51 +1.2 327,221 + SO0320 0.62 +1.6 327,221 + SO0320 0.62 +0.9 327,226 + SO0320 0.63 +1.0 327,226 + SO0320 0.63 +0.5 327,226 + SO0320 0.63 -0.1 327,229 - SO0320 0.63 +1.4 327,229 - SO0320 0.63 -0.1 327,229 - SO0320 0.63 -0.8 327,229 - SO0320 0.63 +1.0 327,231 + SO0320 0.63 +0.7 327,361 + SO0320 0.76 +1.5 327,431 + SO0320 0.83 +1.1 327,431 + SO0320 0.83 +0.2 327,431 + SO0320 0.83 -0.0 327,431 + SO0320 0.83 +0.3 327,439 - SO0320 0.84 +0.7 327,439 - SO0320 0.84 +1.3 327,439 - SO0320 0.84 +2.0 327,483 - SO0320 0.88 +0.8 327,621 + +0.9 327,629 - -0.2 327,834 + SO0321 0.22 +1.5 327,931 - SO0321 0.38 +0.9 327,957 + SO0321 0.43 +0.6 327,976 - SO0321 0.46 +0.1 328,157 - SO0321 0.76 +1.9 328,212 + SO0321 0.85 +1.6 328,268 + +0.6 328,308 - -0.2 328,474 + SO0322 0.13 -1.9 328,505 - SO0322 0.18 -0.1 328,530 + SO0322 0.22 -1.3 328,538 - SO0322 0.24 -0.3 328,538 - SO0322 0.24 +2.2 328,540 + SO0322 0.24 -0.3 328,598 + SO0322 0.34 +2.1
Or see this region's nucleotide sequence