Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0154 and SO0157 are separated by 531 nucleotides SO0157 and SO0159 are separated by 476 nucleotides SO0159 and SO0160 are separated by 164 nucleotides
SO0154: SO0154 - conserved hypothetical protein (NCBI ptt file), at 162,366 to 163,490
SO0154
SO0157: SO0157 - proton/glutamate symporter (NCBI ptt file), at 164,022 to 165,260
SO0157
SO0159: SO0159 - hypothetical protein (NCBI ptt file), at 165,737 to 166,093
SO0159
SO0160: SO0160 - transporter, putative (NCBI ptt file), at 166,258 to 167,817
SO0160
Position (kb)
164
165
166 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 163.089 kb on - strand, within SO0154 at 163.145 kb on - strand, within SO0154 at 163.287 kb on + strand, within SO0154 at 163.307 kb on + strand, within SO0154 at 163.403 kb on + strand at 163.411 kb on - strand at 163.415 kb on + strand at 163.423 kb on - strand at 163.833 kb on + strand at 163.873 kb on + strand at 163.881 kb on - strand at 163.887 kb on - strand at 163.902 kb on + strand at 163.902 kb on + strand at 163.906 kb on + strand at 163.915 kb on - strand at 164.001 kb on + strand at 164.001 kb on + strand at 164.003 kb on - strand at 164.003 kb on - strand at 164.178 kb on + strand, within SO0157 at 164.211 kb on + strand, within SO0157 at 164.272 kb on - strand, within SO0157 at 164.370 kb on - strand, within SO0157 at 164.407 kb on - strand, within SO0157 at 164.407 kb on - strand, within SO0157 at 164.407 kb on - strand, within SO0157 at 164.542 kb on - strand, within SO0157 at 164.545 kb on - strand, within SO0157 at 164.577 kb on + strand, within SO0157 at 164.650 kb on - strand, within SO0157 at 164.686 kb on - strand, within SO0157 at 164.719 kb on + strand, within SO0157 at 164.721 kb on + strand, within SO0157 at 164.729 kb on - strand, within SO0157 at 164.729 kb on - strand, within SO0157 at 164.729 kb on - strand, within SO0157 at 164.731 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.755 kb on + strand, within SO0157 at 164.761 kb on + strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.763 kb on - strand, within SO0157 at 164.766 kb on + strand, within SO0157 at 164.840 kb on + strand, within SO0157 at 164.844 kb on + strand, within SO0157 at 164.844 kb on + strand, within SO0157 at 164.858 kb on + strand, within SO0157 at 164.876 kb on - strand, within SO0157 at 164.909 kb on - strand, within SO0157 at 164.925 kb on + strand, within SO0157 at 164.939 kb on + strand, within SO0157 at 164.948 kb on - strand, within SO0157 at 164.948 kb on - strand, within SO0157 at 164.999 kb on + strand, within SO0157 at 164.999 kb on + strand, within SO0157 at 165.007 kb on - strand, within SO0157 at 165.007 kb on - strand, within SO0157 at 165.007 kb on - strand, within SO0157 at 165.035 kb on + strand, within SO0157 at 165.035 kb on + strand, within SO0157 at 165.062 kb on + strand, within SO0157 at 165.101 kb on - strand, within SO0157 at 165.346 kb on - strand at 165.346 kb on - strand at 165.462 kb on - strand at 165.512 kb on + strand at 165.576 kb on + strand at 165.576 kb on + strand at 165.578 kb on + strand at 165.578 kb on + strand at 165.584 kb on - strand at 165.584 kb on - strand at 165.586 kb on - strand at 165.586 kb on - strand at 165.586 kb on - strand at 165.586 kb on - strand at 165.618 kb on + strand at 165.626 kb on - strand at 165.627 kb on + strand at 165.635 kb on + strand at 165.635 kb on - strand at 165.669 kb on + strand at 165.670 kb on + strand at 165.670 kb on + strand at 165.670 kb on + strand at 165.670 kb on + strand at 165.670 kb on + strand at 165.678 kb on - strand at 165.678 kb on - strand at 165.692 kb on - strand at 165.778 kb on + strand, within SO0159 at 165.778 kb on + strand, within SO0159 at 165.778 kb on - strand, within SO0159 at 165.783 kb on + strand, within SO0159 at 165.938 kb on + strand, within SO0159 at 165.938 kb on + strand, within SO0159 at 166.027 kb on - strand, within SO0159 at 166.040 kb on - strand, within SO0159 at 166.050 kb on - strand, within SO0159 at 166.160 kb on + strand at 166.188 kb on + strand at 166.190 kb on + strand at 166.190 kb on + strand at 166.190 kb on + strand at 166.198 kb on - strand at 166.198 kb on - strand at 166.254 kb on - strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand at 166.256 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 163,089 - SO0154 0.64 -1.6 163,145 - SO0154 0.69 +0.2 163,287 + SO0154 0.82 -1.7 163,307 + SO0154 0.84 -0.2 163,403 + +1.3 163,411 - +0.4 163,415 + +1.3 163,423 - +0.2 163,833 + +0.5 163,873 + -1.2 163,881 - +1.2 163,887 - +0.4 163,902 + +0.1 163,902 + +0.2 163,906 + -0.3 163,915 - +0.2 164,001 + -0.2 164,001 + -0.4 164,003 - -1.3 164,003 - +0.9 164,178 + SO0157 0.13 -0.3 164,211 + SO0157 0.15 -1.5 164,272 - SO0157 0.20 +0.3 164,370 - SO0157 0.28 -0.1 164,407 - SO0157 0.31 -1.2 164,407 - SO0157 0.31 -0.3 164,407 - SO0157 0.31 +0.1 164,542 - SO0157 0.42 +0.1 164,545 - SO0157 0.42 +0.0 164,577 + SO0157 0.45 +1.3 164,650 - SO0157 0.51 -0.5 164,686 - SO0157 0.54 -0.5 164,719 + SO0157 0.56 -1.7 164,721 + SO0157 0.56 +0.4 164,729 - SO0157 0.57 +0.3 164,729 - SO0157 0.57 +0.8 164,729 - SO0157 0.57 -0.6 164,731 + SO0157 0.57 -0.4 164,755 + SO0157 0.59 -0.0 164,755 + SO0157 0.59 -0.5 164,755 + SO0157 0.59 -0.6 164,755 + SO0157 0.59 -0.7 164,755 + SO0157 0.59 +0.7 164,755 + SO0157 0.59 +0.6 164,755 + SO0157 0.59 -1.2 164,755 + SO0157 0.59 -2.3 164,755 + SO0157 0.59 +0.0 164,755 + SO0157 0.59 -0.7 164,755 + SO0157 0.59 +0.3 164,761 + SO0157 0.60 +0.6 164,763 - SO0157 0.60 +0.5 164,763 - SO0157 0.60 +0.2 164,763 - SO0157 0.60 -1.1 164,763 - SO0157 0.60 -0.8 164,763 - SO0157 0.60 -0.2 164,763 - SO0157 0.60 +0.5 164,763 - SO0157 0.60 +0.7 164,763 - SO0157 0.60 -0.7 164,763 - SO0157 0.60 -0.3 164,766 + SO0157 0.60 -1.1 164,840 + SO0157 0.66 +0.6 164,844 + SO0157 0.66 -0.7 164,844 + SO0157 0.66 -0.7 164,858 + SO0157 0.67 -2.7 164,876 - SO0157 0.69 -0.0 164,909 - SO0157 0.72 +0.5 164,925 + SO0157 0.73 +1.5 164,939 + SO0157 0.74 -0.5 164,948 - SO0157 0.75 +0.2 164,948 - SO0157 0.75 +0.3 164,999 + SO0157 0.79 +0.4 164,999 + SO0157 0.79 +1.0 165,007 - SO0157 0.79 +0.6 165,007 - SO0157 0.79 +0.0 165,007 - SO0157 0.79 -0.5 165,035 + SO0157 0.82 -0.9 165,035 + SO0157 0.82 -0.4 165,062 + SO0157 0.84 -1.9 165,101 - SO0157 0.87 +1.1 165,346 - -0.4 165,346 - -2.0 165,462 - -0.0 165,512 + +0.1 165,576 + +1.7 165,576 + -2.0 165,578 + +1.3 165,578 + +0.1 165,584 - +0.3 165,584 - +0.5 165,586 - +0.8 165,586 - -0.0 165,586 - -1.5 165,586 - -0.4 165,618 + +3.1 165,626 - -0.6 165,627 + -0.1 165,635 + +0.6 165,635 - +0.2 165,669 + +0.1 165,670 + -0.7 165,670 + +1.8 165,670 + +0.9 165,670 + -0.1 165,670 + -1.1 165,678 - +0.6 165,678 - -0.0 165,692 - -0.8 165,778 + SO0159 0.11 +0.2 165,778 + SO0159 0.11 +1.0 165,778 - SO0159 0.11 -0.2 165,783 + SO0159 0.13 +1.4 165,938 + SO0159 0.56 -1.3 165,938 + SO0159 0.56 +0.6 166,027 - SO0159 0.81 +1.1 166,040 - SO0159 0.85 -1.7 166,050 - SO0159 0.88 -1.5 166,160 + +0.3 166,188 + +0.9 166,190 + -0.0 166,190 + -1.9 166,190 + -0.8 166,198 - +0.6 166,198 - +0.7 166,254 - +0.3 166,256 + +0.4 166,256 + -0.2 166,256 + +3.0 166,256 + +0.1 166,256 + -2.0 166,256 + +1.0 166,256 + +0.2 166,256 + +2.1 166,256 + -0.2 166,256 + -0.2
Or see this region's nucleotide sequence