Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0121 and SO0122 overlap by 4 nucleotides SO0122 and SO0123 are separated by 26 nucleotides SO0123 and SO0125 are separated by 211 nucleotides SO0125 and SO0126 are separated by 21 nucleotides
SO0121: SO0121 - hypothetical Small-conductance mechanosensitive channel (NCBI ptt file), at 130,527 to 131,705
SO0121
SO0122: SO0122 - transporter, LysE family (NCBI ptt file), at 131,702 to 132,325
SO0122
SO0123: SO0123 - acyltransferase family protein (NCBI ptt file), at 132,352 to 132,912
SO0123
SO0125: SO0125 - conserved hypothetical protein (NCBI ptt file), at 133,124 to 133,489
SO0125
SO0126: SO0126 - conserved hypothetical protein (NCBI ptt file), at 133,511 to 134,311
SO0126
Position (kb) 132
133 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 131.423 kb on + strand, within SO0121 at 131.484 kb on + strand, within SO0121 at 131.499 kb on - strand, within SO0121 at 131.506 kb on + strand, within SO0121 at 131.534 kb on - strand, within SO0121 at 131.644 kb on + strand at 131.701 kb on - strand at 131.707 kb on - strand at 131.714 kb on + strand at 131.766 kb on - strand, within SO0122 at 131.830 kb on + strand, within SO0122 at 131.837 kb on + strand, within SO0122 at 131.845 kb on - strand, within SO0122 at 131.866 kb on - strand, within SO0122 at 131.871 kb on - strand, within SO0122 at 131.918 kb on + strand, within SO0122 at 131.942 kb on + strand, within SO0122 at 131.942 kb on + strand, within SO0122 at 131.950 kb on - strand, within SO0122 at 132.022 kb on + strand, within SO0122 at 132.030 kb on - strand, within SO0122 at 132.121 kb on - strand, within SO0122 at 132.173 kb on - strand, within SO0122 at 132.194 kb on - strand, within SO0122 at 132.234 kb on - strand, within SO0122 at 132.402 kb on + strand at 132.410 kb on - strand, within SO0123 at 132.410 kb on - strand, within SO0123 at 132.410 kb on - strand, within SO0123 at 132.410 kb on - strand, within SO0123 at 132.460 kb on + strand, within SO0123 at 132.468 kb on - strand, within SO0123 at 132.518 kb on + strand, within SO0123 at 132.526 kb on - strand, within SO0123 at 132.746 kb on - strand, within SO0123 at 132.805 kb on + strand, within SO0123 at 132.895 kb on + strand at 132.903 kb on - strand at 132.929 kb on - strand at 132.986 kb on + strand at 132.995 kb on - strand at 133.002 kb on + strand at 133.010 kb on - strand at 133.010 kb on - strand at 133.035 kb on + strand at 133.043 kb on - strand at 133.114 kb on + strand at 133.114 kb on + strand at 133.122 kb on - strand at 133.122 kb on - strand at 133.122 kb on - strand at 133.225 kb on + strand, within SO0125 at 133.233 kb on - strand, within SO0125 at 133.233 kb on - strand, within SO0125 at 133.242 kb on - strand, within SO0125 at 133.265 kb on + strand, within SO0125 at 133.265 kb on + strand, within SO0125 at 133.273 kb on - strand, within SO0125 at 133.317 kb on - strand, within SO0125 at 133.410 kb on + strand, within SO0125 at 133.410 kb on + strand, within SO0125 at 133.421 kb on + strand, within SO0125 at 133.467 kb on + strand at 133.487 kb on - strand at 133.587 kb on + strand at 133.592 kb on + strand, within SO0126 at 133.732 kb on - strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.805 kb on - strand, within SO0126 at 133.805 kb on - strand, within SO0126 at 133.846 kb on - strand, within SO0126
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 131,423 + SO0121 0.76 -1.0 131,484 + SO0121 0.81 +0.1 131,499 - SO0121 0.82 -0.4 131,506 + SO0121 0.83 -0.7 131,534 - SO0121 0.85 -0.3 131,644 + +1.3 131,701 - +0.5 131,707 - -1.5 131,714 + -1.3 131,766 - SO0122 0.10 +3.1 131,830 + SO0122 0.21 +1.3 131,837 + SO0122 0.22 -0.7 131,845 - SO0122 0.23 -0.4 131,866 - SO0122 0.26 +2.1 131,871 - SO0122 0.27 -0.1 131,918 + SO0122 0.35 -2.1 131,942 + SO0122 0.38 -0.7 131,942 + SO0122 0.38 +0.7 131,950 - SO0122 0.40 +0.9 132,022 + SO0122 0.51 +0.1 132,030 - SO0122 0.53 +1.1 132,121 - SO0122 0.67 -2.5 132,173 - SO0122 0.75 +0.5 132,194 - SO0122 0.79 -0.3 132,234 - SO0122 0.85 -0.6 132,402 + -0.1 132,410 - SO0123 0.10 -5.0 132,410 - SO0123 0.10 +0.7 132,410 - SO0123 0.10 -0.5 132,410 - SO0123 0.10 +0.4 132,460 + SO0123 0.19 -0.5 132,468 - SO0123 0.21 -1.7 132,518 + SO0123 0.30 -1.7 132,526 - SO0123 0.31 -1.8 132,746 - SO0123 0.70 -0.7 132,805 + SO0123 0.81 +0.1 132,895 + +0.3 132,903 - -0.2 132,929 - -2.3 132,986 + -0.5 132,995 - +0.7 133,002 + -0.2 133,010 - +0.3 133,010 - -0.1 133,035 + +0.8 133,043 - +0.8 133,114 + -1.9 133,114 + +0.3 133,122 - -0.6 133,122 - +0.2 133,122 - -0.7 133,225 + SO0125 0.28 +0.3 133,233 - SO0125 0.30 +1.9 133,233 - SO0125 0.30 +1.0 133,242 - SO0125 0.32 +0.8 133,265 + SO0125 0.39 -1.3 133,265 + SO0125 0.39 +0.8 133,273 - SO0125 0.41 -0.1 133,317 - SO0125 0.53 +0.5 133,410 + SO0125 0.78 -0.9 133,410 + SO0125 0.78 -0.4 133,421 + SO0125 0.81 -1.9 133,467 + -0.5 133,487 - -1.0 133,587 + -0.2 133,592 + SO0126 0.10 +0.3 133,732 - SO0126 0.28 +0.7 133,797 + SO0126 0.36 +0.7 133,797 + SO0126 0.36 +0.2 133,797 + SO0126 0.36 +0.2 133,797 + SO0126 0.36 +0.8 133,797 + SO0126 0.36 -1.4 133,805 - SO0126 0.37 +0.2 133,805 - SO0126 0.37 +0.2 133,846 - SO0126 0.42 +0.8
Or see this region's nucleotide sequence