Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0067 and SO0068 are separated by 24 nucleotides SO0068 and SO0069 are separated by 57 nucleotides SO0069 and natA are separated by 97 nucleotides natA and SO0071 are separated by 2 nucleotides
SO0067: SO0067 - penicillin-binding protein 1C, putative (NCBI ptt file), at 78,057 to 80,339
SO0067
SO0068: SO0068 - hypothetical protein (NCBI ptt file), at 80,364 to 80,609
SO0068
SO0069: SO0069 - transport protein, putative (NCBI ptt file), at 80,667 to 81,806
SO0069
SO0070: natA - ATP-binding transport protein NatA (NCBI ptt file), at 81,904 to 82,638
natA
SO0071: SO0071 - hydrolase, alpha/beta hydrolase fold family (NCBI ptt file), at 82,641 to 84,260
SO0071
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 79.755 kb on + strand, within SO0067 at 79.755 kb on + strand, within SO0067 at 79.827 kb on - strand, within SO0067 at 79.827 kb on - strand, within SO0067 at 79.840 kb on + strand, within SO0067 at 79.890 kb on + strand, within SO0067 at 79.906 kb on - strand, within SO0067 at 79.933 kb on + strand, within SO0067 at 79.967 kb on + strand, within SO0067 at 80.003 kb on + strand, within SO0067 at 80.071 kb on - strand, within SO0067 at 80.074 kb on - strand, within SO0067 at 80.164 kb on - strand at 80.214 kb on + strand at 80.227 kb on - strand at 80.243 kb on - strand at 80.244 kb on - strand at 80.314 kb on + strand at 80.341 kb on - strand at 80.348 kb on - strand at 80.348 kb on - strand at 80.348 kb on - strand at 80.348 kb on - strand at 80.356 kb on - strand at 80.362 kb on + strand at 80.369 kb on - strand at 80.560 kb on - strand, within SO0068 at 80.618 kb on + strand at 80.624 kb on - strand at 80.626 kb on - strand at 80.631 kb on - strand at 80.643 kb on + strand at 80.657 kb on + strand at 80.724 kb on + strand at 80.724 kb on + strand at 80.724 kb on + strand at 80.732 kb on - strand at 80.775 kb on + strand at 80.781 kb on + strand, within SO0069 at 80.783 kb on - strand, within SO0069 at 80.789 kb on - strand, within SO0069 at 80.832 kb on - strand, within SO0069 at 80.838 kb on + strand, within SO0069 at 80.846 kb on - strand, within SO0069 at 80.846 kb on - strand, within SO0069 at 80.880 kb on - strand, within SO0069 at 80.880 kb on - strand, within SO0069 at 80.943 kb on - strand, within SO0069 at 81.021 kb on - strand, within SO0069 at 81.021 kb on - strand, within SO0069 at 81.061 kb on + strand, within SO0069 at 81.062 kb on + strand, within SO0069 at 81.069 kb on + strand, within SO0069 at 81.069 kb on + strand, within SO0069 at 81.069 kb on + strand, within SO0069 at 81.077 kb on - strand, within SO0069 at 81.077 kb on - strand, within SO0069 at 81.170 kb on + strand, within SO0069 at 81.196 kb on - strand, within SO0069 at 81.204 kb on - strand, within SO0069 at 81.220 kb on - strand, within SO0069 at 81.237 kb on + strand, within SO0069 at 81.249 kb on + strand, within SO0069 at 81.257 kb on - strand, within SO0069 at 81.257 kb on - strand, within SO0069 at 81.257 kb on - strand, within SO0069 at 81.267 kb on - strand, within SO0069 at 81.313 kb on + strand, within SO0069 at 81.321 kb on - strand, within SO0069 at 81.406 kb on + strand, within SO0069 at 81.414 kb on - strand, within SO0069 at 81.414 kb on - strand, within SO0069 at 81.498 kb on + strand, within SO0069 at 81.521 kb on + strand, within SO0069 at 81.529 kb on - strand, within SO0069 at 81.555 kb on - strand, within SO0069 at 81.581 kb on - strand, within SO0069 at 81.586 kb on - strand, within SO0069 at 81.666 kb on - strand, within SO0069 at 81.697 kb on + strand at 81.705 kb on - strand at 81.729 kb on - strand at 81.771 kb on - strand at 81.771 kb on - strand at 81.876 kb on + strand at 81.919 kb on - strand at 81.939 kb on - strand at 81.964 kb on - strand at 81.977 kb on + strand at 81.982 kb on + strand, within natA at 82.008 kb on + strand, within natA at 82.010 kb on + strand, within natA at 82.015 kb on + strand, within natA at 82.015 kb on + strand, within natA at 82.023 kb on - strand, within natA at 82.035 kb on + strand, within natA at 82.124 kb on - strand, within natA at 82.146 kb on + strand, within natA at 82.192 kb on + strand, within natA at 82.376 kb on - strand, within natA at 82.376 kb on - strand, within natA at 82.427 kb on - strand, within natA at 82.489 kb on - strand, within natA at 82.534 kb on - strand, within natA at 82.591 kb on + strand at 82.599 kb on - strand at 82.665 kb on - strand at 82.677 kb on - strand at 82.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 79,755 + SO0067 0.74 +1.3 79,755 + SO0067 0.74 -0.7 79,827 - SO0067 0.78 -0.7 79,827 - SO0067 0.78 +1.0 79,840 + SO0067 0.78 -0.6 79,890 + SO0067 0.80 -0.7 79,906 - SO0067 0.81 +0.4 79,933 + SO0067 0.82 +1.7 79,967 + SO0067 0.84 -0.3 80,003 + SO0067 0.85 -0.1 80,071 - SO0067 0.88 -1.9 80,074 - SO0067 0.88 +1.3 80,164 - -0.9 80,214 + -0.0 80,227 - +1.2 80,243 - +0.3 80,244 - -0.2 80,314 + -0.5 80,341 - -0.9 80,348 - +0.6 80,348 - +1.2 80,348 - +0.6 80,348 - +1.2 80,356 - -0.0 80,362 + +1.1 80,369 - -2.7 80,560 - SO0068 0.80 -0.9 80,618 + +0.5 80,624 - +0.6 80,626 - -0.3 80,631 - -0.4 80,643 + -0.3 80,657 + +1.7 80,724 + +0.7 80,724 + -0.4 80,724 + +0.4 80,732 - -0.0 80,775 + +0.3 80,781 + SO0069 0.10 +0.4 80,783 - SO0069 0.10 -1.3 80,789 - SO0069 0.11 +0.7 80,832 - SO0069 0.14 -0.4 80,838 + SO0069 0.15 +0.5 80,846 - SO0069 0.16 +0.4 80,846 - SO0069 0.16 +0.8 80,880 - SO0069 0.19 -1.1 80,880 - SO0069 0.19 -0.7 80,943 - SO0069 0.24 -0.1 81,021 - SO0069 0.31 -0.6 81,021 - SO0069 0.31 -0.3 81,061 + SO0069 0.35 -0.4 81,062 + SO0069 0.35 +0.2 81,069 + SO0069 0.35 -0.4 81,069 + SO0069 0.35 -0.4 81,069 + SO0069 0.35 +0.1 81,077 - SO0069 0.36 -1.0 81,077 - SO0069 0.36 -3.5 81,170 + SO0069 0.44 +0.5 81,196 - SO0069 0.46 +1.3 81,204 - SO0069 0.47 +0.3 81,220 - SO0069 0.49 +1.1 81,237 + SO0069 0.50 -0.8 81,249 + SO0069 0.51 -0.8 81,257 - SO0069 0.52 -0.4 81,257 - SO0069 0.52 -0.0 81,257 - SO0069 0.52 +0.3 81,267 - SO0069 0.53 +0.7 81,313 + SO0069 0.57 +0.5 81,321 - SO0069 0.57 +1.2 81,406 + SO0069 0.65 -0.3 81,414 - SO0069 0.66 -1.0 81,414 - SO0069 0.66 +0.2 81,498 + SO0069 0.73 -1.9 81,521 + SO0069 0.75 -0.1 81,529 - SO0069 0.76 -2.6 81,555 - SO0069 0.78 -1.6 81,581 - SO0069 0.80 -1.5 81,586 - SO0069 0.81 +0.1 81,666 - SO0069 0.88 +0.1 81,697 + +0.1 81,705 - -0.2 81,729 - +1.3 81,771 - -0.3 81,771 - -1.2 81,876 + -0.4 81,919 - -0.8 81,939 - -0.9 81,964 - -3.2 81,977 + -2.6 81,982 + natA SO0070 0.11 -0.3 82,008 + natA SO0070 0.14 +0.4 82,010 + natA SO0070 0.14 -0.0 82,015 + natA SO0070 0.15 -1.9 82,015 + natA SO0070 0.15 -0.0 82,023 - natA SO0070 0.16 -0.4 82,035 + natA SO0070 0.18 -0.2 82,124 - natA SO0070 0.30 -0.3 82,146 + natA SO0070 0.33 -0.5 82,192 + natA SO0070 0.39 -0.4 82,376 - natA SO0070 0.64 -0.5 82,376 - natA SO0070 0.64 -0.3 82,427 - natA SO0070 0.71 -0.6 82,489 - natA SO0070 0.80 +0.2 82,534 - natA SO0070 0.86 +0.9 82,591 + -0.3 82,599 - -0.7 82,665 - -1.3 82,677 - -1.4 82,711 - -1.7
Or see this region's nucleotide sequence