Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trkA and sun are separated by 15 nucleotides sun and fmt are separated by 2 nucleotides fmt and def-1 are separated by 15 nucleotides
SO0029: trkA - potassium uptake protein TrkA (NCBI ptt file), at 33,706 to 35,115
trkA
SO0030: sun - sun protein (NCBI ptt file), at 35,131 to 36,417
sun
SO0031: fmt - methionyl-tRNA formyltransferase (NCBI ptt file), at 36,420 to 37,376
fmt
SO0032: def-1 - polypeptide deformylase (NCBI ptt file), at 37,392 to 37,898
def-1
Position (kb) 35
36
37 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 34.621 kb on + strand, within trkA at 35.212 kb on - strand at 35.242 kb on - strand at 35.242 kb on - strand at 35.243 kb on + strand at 35.286 kb on - strand, within sun at 35.301 kb on + strand, within sun at 35.663 kb on - strand, within sun at 35.730 kb on + strand, within sun at 35.740 kb on + strand, within sun at 35.764 kb on - strand, within sun at 35.843 kb on + strand, within sun at 35.843 kb on + strand, within sun at 35.915 kb on - strand, within sun at 35.922 kb on + strand, within sun at 35.937 kb on + strand, within sun at 35.937 kb on + strand, within sun at 36.046 kb on + strand, within sun at 36.068 kb on + strand, within sun at 36.120 kb on - strand, within sun at 36.142 kb on + strand, within sun at 36.190 kb on - strand, within sun at 36.227 kb on + strand, within sun at 36.276 kb on + strand, within sun at 36.286 kb on + strand, within sun at 36.286 kb on - strand, within sun at 36.303 kb on - strand at 36.359 kb on - strand at 36.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 34,621 + trkA SO0029 0.65 -0.7 35,212 - -1.0 35,242 - -3.9 35,242 - -2.7 35,243 + -3.1 35,286 - sun SO0030 0.12 -0.4 35,301 + sun SO0030 0.13 -2.1 35,663 - sun SO0030 0.41 -1.2 35,730 + sun SO0030 0.47 -2.3 35,740 + sun SO0030 0.47 -1.5 35,764 - sun SO0030 0.49 -0.5 35,843 + sun SO0030 0.55 -1.3 35,843 + sun SO0030 0.55 -1.5 35,915 - sun SO0030 0.61 -2.5 35,922 + sun SO0030 0.61 +1.3 35,937 + sun SO0030 0.63 -0.2 35,937 + sun SO0030 0.63 -3.2 36,046 + sun SO0030 0.71 -1.0 36,068 + sun SO0030 0.73 -0.3 36,120 - sun SO0030 0.77 -3.0 36,142 + sun SO0030 0.79 +1.1 36,190 - sun SO0030 0.82 -2.3 36,227 + sun SO0030 0.85 -2.3 36,276 + sun SO0030 0.89 -1.7 36,286 + sun SO0030 0.90 -2.9 36,286 - sun SO0030 0.90 +1.3 36,303 - -1.7 36,359 - -1.9 36,460 - -0.6
Or see this region's nucleotide sequence