Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepQ and SO0023 are separated by 15 nucleotides SO0023 and trkH-1 are separated by 77 nucleotides trkH-1 and SO0025 are separated by 1 nucleotides SO0025 and SO0026 are separated by 64 nucleotides
SO0022: pepQ - prolidase (NCBI ptt file), at 27,069 to 28,388
pepQ
SO0023: SO0023 - conserved hypothetical protein TIGR00257 (NCBI ptt file), at 28,404 to 29,018
SO0023
SO0024: trkH-1 - potassium uptake protein TrkH (NCBI ptt file), at 29,096 to 30,556
trkH-1
SO0025: SO0025 - hypothetical flavodoxin (NCBI ptt file), at 30,558 to 31,082
SO0025
SO0026: SO0026 - transcriptional regulator, ArsR family (NCBI ptt file), at 31,147 to 31,455
SO0026
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 28.111 kb on + strand, within pepQ at 28.137 kb on - strand, within pepQ at 28.141 kb on + strand, within pepQ at 28.212 kb on + strand, within pepQ at 28.293 kb on - strand at 28.303 kb on - strand at 28.354 kb on - strand at 28.388 kb on + strand at 28.414 kb on - strand at 28.414 kb on - strand at 28.417 kb on + strand at 28.458 kb on + strand at 28.463 kb on - strand at 28.469 kb on + strand, within SO0023 at 28.498 kb on + strand, within SO0023 at 28.538 kb on + strand, within SO0023 at 28.617 kb on - strand, within SO0023 at 28.647 kb on + strand, within SO0023 at 28.688 kb on + strand, within SO0023 at 28.698 kb on + strand, within SO0023 at 28.720 kb on + strand, within SO0023 at 28.728 kb on - strand, within SO0023 at 28.740 kb on + strand, within SO0023 at 28.759 kb on + strand, within SO0023 at 28.759 kb on + strand, within SO0023 at 28.759 kb on + strand, within SO0023 at 28.764 kb on + strand, within SO0023 at 28.778 kb on - strand, within SO0023 at 28.814 kb on - strand, within SO0023 at 28.962 kb on + strand at 28.965 kb on + strand at 28.977 kb on + strand at 28.977 kb on + strand at 28.977 kb on + strand at 28.977 kb on + strand at 28.983 kb on + strand at 28.985 kb on - strand at 28.986 kb on - strand at 28.991 kb on - strand at 29.005 kb on + strand at 29.005 kb on + strand at 29.096 kb on + strand at 29.096 kb on + strand at 29.143 kb on + strand at 29.251 kb on + strand, within trkH-1 at 29.320 kb on + strand, within trkH-1 at 29.320 kb on + strand, within trkH-1 at 29.320 kb on + strand, within trkH-1 at 29.320 kb on + strand, within trkH-1 at 29.320 kb on + strand, within trkH-1 at 29.320 kb on + strand, within trkH-1 at 29.328 kb on - strand, within trkH-1 at 29.328 kb on - strand, within trkH-1 at 29.328 kb on - strand, within trkH-1 at 29.328 kb on - strand, within trkH-1 at 29.328 kb on - strand, within trkH-1 at 29.328 kb on - strand, within trkH-1 at 29.330 kb on + strand, within trkH-1 at 29.330 kb on + strand, within trkH-1 at 29.330 kb on + strand, within trkH-1 at 29.338 kb on - strand, within trkH-1 at 29.338 kb on - strand, within trkH-1 at 29.338 kb on - strand, within trkH-1 at 29.378 kb on + strand, within trkH-1 at 29.422 kb on + strand, within trkH-1 at 29.430 kb on - strand, within trkH-1 at 29.430 kb on - strand, within trkH-1 at 29.435 kb on - strand, within trkH-1 at 29.452 kb on - strand, within trkH-1 at 29.458 kb on + strand, within trkH-1 at 29.458 kb on - strand, within trkH-1 at 29.549 kb on + strand, within trkH-1 at 29.580 kb on - strand, within trkH-1 at 29.627 kb on - strand, within trkH-1 at 29.652 kb on - strand, within trkH-1 at 29.858 kb on - strand, within trkH-1 at 29.886 kb on + strand, within trkH-1 at 29.904 kb on + strand, within trkH-1 at 29.912 kb on - strand, within trkH-1 at 29.919 kb on + strand, within trkH-1 at 30.015 kb on + strand, within trkH-1 at 30.023 kb on - strand, within trkH-1 at 30.062 kb on + strand, within trkH-1 at 30.064 kb on + strand, within trkH-1 at 30.072 kb on - strand, within trkH-1 at 30.162 kb on + strand, within trkH-1 at 30.162 kb on + strand, within trkH-1 at 30.162 kb on + strand, within trkH-1 at 30.163 kb on + strand, within trkH-1 at 30.163 kb on + strand, within trkH-1 at 30.171 kb on - strand, within trkH-1 at 30.171 kb on - strand, within trkH-1 at 30.171 kb on - strand, within trkH-1 at 30.210 kb on + strand, within trkH-1 at 30.334 kb on + strand at 30.419 kb on + strand at 30.426 kb on + strand at 30.427 kb on - strand at 30.428 kb on - strand at 30.432 kb on - strand at 30.450 kb on + strand at 30.463 kb on + strand at 30.483 kb on + strand at 30.486 kb on - strand at 30.486 kb on - strand at 30.617 kb on + strand, within SO0025 at 30.664 kb on + strand, within SO0025 at 30.664 kb on + strand, within SO0025 at 30.664 kb on + strand, within SO0025 at 30.664 kb on + strand, within SO0025 at 30.664 kb on + strand, within SO0025 at 30.672 kb on - strand, within SO0025 at 30.677 kb on - strand, within SO0025 at 30.681 kb on + strand, within SO0025 at 30.681 kb on + strand, within SO0025 at 30.681 kb on + strand, within SO0025 at 30.681 kb on + strand, within SO0025 at 30.689 kb on + strand, within SO0025 at 30.689 kb on - strand, within SO0025 at 30.689 kb on - strand, within SO0025 at 30.689 kb on - strand, within SO0025 at 30.689 kb on - strand, within SO0025 at 30.689 kb on - strand, within SO0025 at 30.707 kb on + strand, within SO0025 at 30.735 kb on + strand, within SO0025 at 30.743 kb on - strand, within SO0025 at 30.743 kb on - strand, within SO0025 at 30.745 kb on + strand, within SO0025 at 30.748 kb on - strand, within SO0025 at 30.753 kb on - strand, within SO0025 at 30.909 kb on + strand, within SO0025 at 30.984 kb on - strand, within SO0025 at 31.029 kb on - strand, within SO0025 at 31.029 kb on - strand, within SO0025 at 31.099 kb on + strand at 31.106 kb on - strand at 31.126 kb on + strand at 31.134 kb on - strand at 31.152 kb on - strand at 31.173 kb on - strand at 31.210 kb on - strand, within SO0026 at 31.232 kb on + strand, within SO0026 at 31.269 kb on + strand, within SO0026 at 31.338 kb on - strand, within SO0026 at 31.388 kb on + strand, within SO0026 at 31.516 kb on + strand at 31.516 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 28,111 + pepQ SO0022 0.79 +0.7 28,137 - pepQ SO0022 0.81 -0.5 28,141 + pepQ SO0022 0.81 +0.2 28,212 + pepQ SO0022 0.87 -0.3 28,293 - +1.3 28,303 - -0.8 28,354 - -0.3 28,388 + +0.3 28,414 - -0.8 28,414 - +1.5 28,417 + -3.4 28,458 + +1.1 28,463 - +0.6 28,469 + SO0023 0.11 -0.8 28,498 + SO0023 0.15 -1.0 28,538 + SO0023 0.22 +0.1 28,617 - SO0023 0.35 -1.7 28,647 + SO0023 0.40 +0.6 28,688 + SO0023 0.46 +0.1 28,698 + SO0023 0.48 -1.1 28,720 + SO0023 0.51 +0.1 28,728 - SO0023 0.53 -1.0 28,740 + SO0023 0.55 +0.5 28,759 + SO0023 0.58 +0.3 28,759 + SO0023 0.58 -0.6 28,759 + SO0023 0.58 -0.6 28,764 + SO0023 0.59 +0.4 28,778 - SO0023 0.61 -0.3 28,814 - SO0023 0.67 -1.8 28,962 + +1.0 28,965 + +0.8 28,977 + -0.1 28,977 + +0.1 28,977 + +0.3 28,977 + +0.4 28,983 + +0.9 28,985 - -0.8 28,986 - +0.4 28,991 - +0.5 29,005 + +0.1 29,005 + -2.4 29,096 + +0.6 29,096 + -0.5 29,143 + -2.9 29,251 + trkH-1 SO0024 0.11 -1.9 29,320 + trkH-1 SO0024 0.15 -2.3 29,320 + trkH-1 SO0024 0.15 -0.8 29,320 + trkH-1 SO0024 0.15 -0.9 29,320 + trkH-1 SO0024 0.15 -1.1 29,320 + trkH-1 SO0024 0.15 -1.2 29,320 + trkH-1 SO0024 0.15 -0.6 29,328 - trkH-1 SO0024 0.16 -1.1 29,328 - trkH-1 SO0024 0.16 -1.3 29,328 - trkH-1 SO0024 0.16 -1.3 29,328 - trkH-1 SO0024 0.16 -0.7 29,328 - trkH-1 SO0024 0.16 -2.1 29,328 - trkH-1 SO0024 0.16 -1.9 29,330 + trkH-1 SO0024 0.16 -0.3 29,330 + trkH-1 SO0024 0.16 -2.0 29,330 + trkH-1 SO0024 0.16 -1.7 29,338 - trkH-1 SO0024 0.17 -0.4 29,338 - trkH-1 SO0024 0.17 -1.6 29,338 - trkH-1 SO0024 0.17 -1.3 29,378 + trkH-1 SO0024 0.19 -1.4 29,422 + trkH-1 SO0024 0.22 -1.2 29,430 - trkH-1 SO0024 0.23 -1.9 29,430 - trkH-1 SO0024 0.23 -1.7 29,435 - trkH-1 SO0024 0.23 -0.7 29,452 - trkH-1 SO0024 0.24 -0.5 29,458 + trkH-1 SO0024 0.25 -1.2 29,458 - trkH-1 SO0024 0.25 -1.7 29,549 + trkH-1 SO0024 0.31 -2.1 29,580 - trkH-1 SO0024 0.33 -1.7 29,627 - trkH-1 SO0024 0.36 -0.3 29,652 - trkH-1 SO0024 0.38 -2.2 29,858 - trkH-1 SO0024 0.52 -2.3 29,886 + trkH-1 SO0024 0.54 -1.8 29,904 + trkH-1 SO0024 0.55 -2.0 29,912 - trkH-1 SO0024 0.56 +0.1 29,919 + trkH-1 SO0024 0.56 -2.3 30,015 + trkH-1 SO0024 0.63 -2.7 30,023 - trkH-1 SO0024 0.63 -0.9 30,062 + trkH-1 SO0024 0.66 -0.1 30,064 + trkH-1 SO0024 0.66 -2.0 30,072 - trkH-1 SO0024 0.67 -0.3 30,162 + trkH-1 SO0024 0.73 -2.8 30,162 + trkH-1 SO0024 0.73 -3.3 30,162 + trkH-1 SO0024 0.73 -0.9 30,163 + trkH-1 SO0024 0.73 -1.5 30,163 + trkH-1 SO0024 0.73 -2.5 30,171 - trkH-1 SO0024 0.74 -3.1 30,171 - trkH-1 SO0024 0.74 +0.9 30,171 - trkH-1 SO0024 0.74 -1.4 30,210 + trkH-1 SO0024 0.76 +0.5 30,334 + +0.3 30,419 + +0.5 30,426 + -1.0 30,427 - -0.1 30,428 - -2.8 30,432 - -1.4 30,450 + +0.9 30,463 + -1.0 30,483 + -1.5 30,486 - -2.3 30,486 - -1.3 30,617 + SO0025 0.11 -1.3 30,664 + SO0025 0.20 -0.5 30,664 + SO0025 0.20 +0.1 30,664 + SO0025 0.20 +0.3 30,664 + SO0025 0.20 -0.2 30,664 + SO0025 0.20 +0.6 30,672 - SO0025 0.22 -0.5 30,677 - SO0025 0.23 +0.8 30,681 + SO0025 0.23 -0.5 30,681 + SO0025 0.23 +1.0 30,681 + SO0025 0.23 +1.4 30,681 + SO0025 0.23 +0.2 30,689 + SO0025 0.25 +1.1 30,689 - SO0025 0.25 -0.8 30,689 - SO0025 0.25 +0.7 30,689 - SO0025 0.25 +2.3 30,689 - SO0025 0.25 +0.8 30,689 - SO0025 0.25 +1.1 30,707 + SO0025 0.28 -1.7 30,735 + SO0025 0.34 -2.3 30,743 - SO0025 0.35 +1.3 30,743 - SO0025 0.35 -0.3 30,745 + SO0025 0.36 +0.1 30,748 - SO0025 0.36 -0.7 30,753 - SO0025 0.37 +0.1 30,909 + SO0025 0.67 -0.8 30,984 - SO0025 0.81 -0.5 31,029 - SO0025 0.90 +1.6 31,029 - SO0025 0.90 -1.9 31,099 + +0.1 31,106 - +0.1 31,126 + -0.0 31,134 - +0.4 31,152 - +0.4 31,173 - -0.4 31,210 - SO0026 0.20 -2.7 31,232 + SO0026 0.28 -2.4 31,269 + SO0026 0.39 -1.1 31,338 - SO0026 0.62 -2.7 31,388 + SO0026 0.78 -1.3 31,516 + +0.7 31,516 + -0.3
Or see this region's nucleotide sequence