Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1349 and Dshi_1350 are separated by 138 nucleotides Dshi_1350 and Dshi_1351 are separated by 149 nucleotides Dshi_1351 and Dshi_1352 overlap by 4 nucleotides Dshi_1352 and Dshi_1353 are separated by 651 nucleotides
Dshi_1349: Dshi_1349 - ABC transporter related (RefSeq), at 1,400,718 to 1,402,574
_1349
Dshi_1350: Dshi_1350 - Nucleoside-diphosphate kinase (RefSeq), at 1,402,713 to 1,403,135
_1350
Dshi_1351: Dshi_1351 - hypothetical protein (RefSeq), at 1,403,285 to 1,403,677
_1351
Dshi_1352: Dshi_1352 - TfoX domain protein (RefSeq), at 1,403,674 to 1,404,003
_1352
Dshi_1353: Dshi_1353 - extracellular solute-binding protein family 1 (RefSeq), at 1,404,655 to 1,405,959
_1353
Position (kb)
1403
1404 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1402.298 kb on - strand, within Dshi_1349 at 1402.414 kb on - strand at 1402.571 kb on + strand at 1403.282 kb on + strand at 1403.485 kb on - strand, within Dshi_1351 at 1403.553 kb on + strand, within Dshi_1351 at 1403.755 kb on - strand, within Dshi_1352 at 1403.755 kb on - strand, within Dshi_1352 at 1403.937 kb on - strand, within Dshi_1352 at 1404.089 kb on + strand at 1404.089 kb on + strand at 1404.101 kb on + strand at 1404.180 kb on + strand at 1404.188 kb on - strand at 1404.471 kb on - strand at 1404.480 kb on - strand at 1404.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 1,402,298 - Dshi_1349 0.85 -3.0 1,402,414 - -0.8 1,402,571 + -1.2 1,403,282 + +1.2 1,403,485 - Dshi_1351 0.51 -0.2 1,403,553 + Dshi_1351 0.68 +1.8 1,403,755 - Dshi_1352 0.25 +0.9 1,403,755 - Dshi_1352 0.25 -1.4 1,403,937 - Dshi_1352 0.80 +0.6 1,404,089 + +0.4 1,404,089 + +0.2 1,404,101 + -0.3 1,404,180 + +0.4 1,404,188 - +0.2 1,404,471 - -1.7 1,404,480 - +0.1 1,404,595 + -1.8
Or see this region's nucleotide sequence