Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1103 and Dshi_1104 overlap by 4 nucleotides Dshi_1104 and Dshi_1105 are separated by 3 nucleotides Dshi_1105 and Dshi_1106 are separated by 63 nucleotides Dshi_1106 and Dshi_1107 overlap by 4 nucleotides
Dshi_1103: Dshi_1103 - crossover junction endodeoxyribonuclease RuvC (RefSeq), at 1,129,962 to 1,130,492
_1103
Dshi_1104: Dshi_1104 - Holliday junction DNA helicase RuvA (RefSeq), at 1,130,489 to 1,131,163
_1104
Dshi_1105: Dshi_1105 - Holliday junction DNA helicase RuvB (RefSeq), at 1,131,167 to 1,132,240
_1105
Dshi_1106: Dshi_1106 - hypothetical protein (RefSeq), at 1,132,304 to 1,132,930
_1106
Dshi_1107: Dshi_1107 - Pol-Pal system-associated acyl-CoA thioesterase (RefSeq), at 1,132,927 to 1,133,337
_1107
Position (kb)
1131
1132
1133 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1130.191 kb on + strand, within Dshi_1103 at 1130.413 kb on + strand, within Dshi_1103 at 1130.773 kb on + strand, within Dshi_1104 at 1130.890 kb on + strand, within Dshi_1104 at 1131.937 kb on - strand, within Dshi_1105 at 1132.198 kb on + strand at 1132.289 kb on + strand at 1132.290 kb on + strand at 1132.297 kb on - strand at 1132.298 kb on - strand at 1132.298 kb on - strand at 1132.298 kb on - strand at 1132.353 kb on + strand at 1132.374 kb on + strand at 1132.431 kb on - strand, within Dshi_1106 at 1132.594 kb on + strand, within Dshi_1106 at 1132.770 kb on + strand, within Dshi_1106 at 1132.798 kb on + strand, within Dshi_1106 at 1133.018 kb on + strand, within Dshi_1107 at 1133.038 kb on - strand, within Dshi_1107 at 1133.091 kb on - strand, within Dshi_1107 at 1133.148 kb on + strand, within Dshi_1107 at 1133.219 kb on + strand, within Dshi_1107
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 1,130,191 + Dshi_1103 0.43 +0.1 1,130,413 + Dshi_1103 0.85 -1.7 1,130,773 + Dshi_1104 0.42 -1.8 1,130,890 + Dshi_1104 0.59 +0.8 1,131,937 - Dshi_1105 0.72 +0.7 1,132,198 + -0.4 1,132,289 + +0.8 1,132,290 + +0.7 1,132,297 - -0.2 1,132,298 - +0.3 1,132,298 - -0.1 1,132,298 - +1.2 1,132,353 + -0.4 1,132,374 + +2.7 1,132,431 - Dshi_1106 0.20 +0.0 1,132,594 + Dshi_1106 0.46 -0.6 1,132,770 + Dshi_1106 0.74 -1.6 1,132,798 + Dshi_1106 0.79 -0.9 1,133,018 + Dshi_1107 0.22 -1.7 1,133,038 - Dshi_1107 0.27 -0.2 1,133,091 - Dshi_1107 0.40 +1.2 1,133,148 + Dshi_1107 0.54 +0.6 1,133,219 + Dshi_1107 0.71 +0.7
Or see this region's nucleotide sequence