Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0740 and Dshi_0741 are separated by 39 nucleotides Dshi_0741 and Dshi_0742 overlap by 4 nucleotides Dshi_0742 and Dshi_0743 are separated by 162 nucleotides
Dshi_0740: Dshi_0740 - Polyphosphate kinase (RefSeq), at 746,526 to 748,700
_0740
Dshi_0741: Dshi_0741 - chromosomal replication initiator, DnaA (RefSeq), at 748,740 to 749,429
_0741
Dshi_0742: Dshi_0742 - protein of unknown function UPF0118 (RefSeq), at 749,426 to 750,511
_0742
Dshi_0743: Dshi_0743 - MscS Mechanosensitive ion channel (RefSeq), at 750,674 to 752,056
_0743
Position (kb) 749
750
751 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 748.576 kb on - strand at 748.604 kb on + strand at 748.650 kb on - strand at 748.674 kb on - strand at 749.487 kb on + strand at 750.514 kb on - strand at 750.514 kb on - strand at 750.578 kb on - strand at 750.656 kb on - strand at 750.670 kb on + strand at 750.739 kb on + strand at 750.747 kb on - strand at 750.760 kb on - strand at 750.788 kb on - strand at 750.800 kb on + strand at 750.891 kb on + strand, within Dshi_0743 at 750.923 kb on - strand, within Dshi_0743 at 751.014 kb on + strand, within Dshi_0743 at 751.034 kb on + strand, within Dshi_0743 at 751.044 kb on + strand, within Dshi_0743 at 751.129 kb on - strand, within Dshi_0743 at 751.194 kb on - strand, within Dshi_0743 at 751.260 kb on + strand, within Dshi_0743 at 751.261 kb on + strand, within Dshi_0743 at 751.268 kb on - strand, within Dshi_0743 at 751.425 kb on - strand, within Dshi_0743 at 751.479 kb on + strand, within Dshi_0743 at 751.479 kb on + strand, within Dshi_0743 at 751.487 kb on - strand, within Dshi_0743
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 748,576 - -2.0 748,604 + -3.0 748,650 - -0.2 748,674 - -0.5 749,487 + +0.1 750,514 - +0.1 750,514 - -2.4 750,578 - -0.1 750,656 - -0.5 750,670 + +0.7 750,739 + +0.3 750,747 - +1.2 750,760 - -1.9 750,788 - +1.0 750,800 + +0.7 750,891 + Dshi_0743 0.16 -0.0 750,923 - Dshi_0743 0.18 +0.7 751,014 + Dshi_0743 0.25 -0.1 751,034 + Dshi_0743 0.26 +0.2 751,044 + Dshi_0743 0.27 -0.4 751,129 - Dshi_0743 0.33 -1.4 751,194 - Dshi_0743 0.38 +0.8 751,260 + Dshi_0743 0.42 +0.0 751,261 + Dshi_0743 0.42 +0.2 751,268 - Dshi_0743 0.43 +0.1 751,425 - Dshi_0743 0.54 +0.8 751,479 + Dshi_0743 0.58 -1.2 751,479 + Dshi_0743 0.58 -0.3 751,487 - Dshi_0743 0.59 +0.0
Or see this region's nucleotide sequence