Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purH and SO0443 are separated by 335 nucleotides SO0443 and SO0444 overlap by 1 nucleotides SO0444 and SO0445 are separated by 105 nucleotides
SO0442: purH - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI ptt file), at 469,791 to 471,422
purH
SO0443: SO0443 - transcriptional regulator, MerR family (NCBI ptt file), at 471,758 to 472,249
SO0443
SO0444: SO0444 - hypothetical protein (NCBI ptt file), at 472,249 to 473,724
SO0444
SO0445: SO0445 - hflC protein, putative (NCBI ptt file), at 473,830 to 474,720
SO0445
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 471.263 kb on + strand at 471.285 kb on + strand at 471.314 kb on + strand at 471.314 kb on + strand at 471.314 kb on + strand at 471.314 kb on + strand at 471.383 kb on - strand at 471.420 kb on + strand at 471.612 kb on + strand at 471.676 kb on + strand at 471.715 kb on + strand at 471.742 kb on + strand at 471.757 kb on - strand at 471.757 kb on - strand at 471.757 kb on - strand at 471.801 kb on - strand at 471.879 kb on - strand, within SO0443 at 471.879 kb on - strand, within SO0443 at 471.891 kb on + strand, within SO0443 at 471.993 kb on + strand, within SO0443 at 472.006 kb on + strand, within SO0443 at 472.014 kb on - strand, within SO0443 at 472.017 kb on + strand, within SO0443 at 472.286 kb on - strand at 472.383 kb on + strand at 472.391 kb on - strand at 472.456 kb on - strand, within SO0444 at 472.621 kb on + strand, within SO0444 at 472.657 kb on + strand, within SO0444 at 472.698 kb on - strand, within SO0444 at 472.701 kb on + strand, within SO0444 at 472.709 kb on - strand, within SO0444 at 472.711 kb on + strand, within SO0444 at 472.777 kb on + strand, within SO0444 at 472.785 kb on - strand, within SO0444 at 472.963 kb on + strand, within SO0444 at 472.964 kb on + strand, within SO0444 at 472.965 kb on + strand, within SO0444 at 472.983 kb on - strand, within SO0444 at 473.001 kb on - strand, within SO0444 at 473.088 kb on + strand, within SO0444 at 473.202 kb on - strand, within SO0444 at 473.207 kb on + strand, within SO0444 at 473.346 kb on - strand, within SO0444 at 473.348 kb on - strand, within SO0444 at 473.418 kb on - strand, within SO0444 at 473.490 kb on + strand, within SO0444 at 473.490 kb on + strand, within SO0444 at 473.527 kb on - strand, within SO0444 at 473.570 kb on + strand, within SO0444 at 473.578 kb on - strand at 473.602 kb on + strand at 473.653 kb on - strand at 473.653 kb on - strand at 473.793 kb on + strand at 473.923 kb on - strand, within SO0445 at 474.047 kb on + strand, within SO0445 at 474.123 kb on - strand, within SO0445 at 474.195 kb on + strand, within SO0445 at 474.195 kb on + strand, within SO0445 at 474.203 kb on - strand, within SO0445 at 474.203 kb on - strand, within SO0445 at 474.203 kb on - strand, within SO0445 at 474.203 kb on - strand, within SO0445 at 474.213 kb on - strand, within SO0445 at 474.217 kb on + strand, within SO0445 at 474.268 kb on - strand, within SO0445 at 474.279 kb on - strand, within SO0445 at 474.427 kb on - strand, within SO0445 at 474.488 kb on + strand, within SO0445 at 474.488 kb on - strand, within SO0445 at 474.488 kb on - strand, within SO0445 at 474.606 kb on + strand, within SO0445 at 474.614 kb on - strand, within SO0445 at 474.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 471,263 + -2.6 471,285 + -3.7 471,314 + -2.0 471,314 + -3.3 471,314 + -2.6 471,314 + -2.4 471,383 - -3.4 471,420 + -3.2 471,612 + +0.1 471,676 + +0.2 471,715 + +1.4 471,742 + -0.5 471,757 - +0.6 471,757 - -0.2 471,757 - -0.4 471,801 - -0.4 471,879 - SO0443 0.25 -0.0 471,879 - SO0443 0.25 -0.4 471,891 + SO0443 0.27 +0.7 471,993 + SO0443 0.48 +0.8 472,006 + SO0443 0.50 +0.0 472,014 - SO0443 0.52 -0.0 472,017 + SO0443 0.53 +0.2 472,286 - +1.1 472,383 + +1.0 472,391 - -1.0 472,456 - SO0444 0.14 -0.4 472,621 + SO0444 0.25 -0.0 472,657 + SO0444 0.28 +0.4 472,698 - SO0444 0.30 -0.6 472,701 + SO0444 0.31 +0.6 472,709 - SO0444 0.31 -0.2 472,711 + SO0444 0.31 +1.0 472,777 + SO0444 0.36 +0.5 472,785 - SO0444 0.36 -0.2 472,963 + SO0444 0.48 -0.7 472,964 + SO0444 0.48 +1.1 472,965 + SO0444 0.49 -0.3 472,983 - SO0444 0.50 +1.4 473,001 - SO0444 0.51 -0.2 473,088 + SO0444 0.57 +2.9 473,202 - SO0444 0.65 -0.8 473,207 + SO0444 0.65 -0.0 473,346 - SO0444 0.74 -0.3 473,348 - SO0444 0.74 +0.8 473,418 - SO0444 0.79 -0.1 473,490 + SO0444 0.84 -0.1 473,490 + SO0444 0.84 -0.5 473,527 - SO0444 0.87 +0.1 473,570 + SO0444 0.89 +0.1 473,578 - -0.1 473,602 + -0.4 473,653 - -0.1 473,653 - +0.4 473,793 + +0.1 473,923 - SO0445 0.10 -1.4 474,047 + SO0445 0.24 -0.3 474,123 - SO0445 0.33 -0.4 474,195 + SO0445 0.41 +0.8 474,195 + SO0445 0.41 -0.6 474,203 - SO0445 0.42 +0.2 474,203 - SO0445 0.42 +0.0 474,203 - SO0445 0.42 +0.2 474,203 - SO0445 0.42 +0.3 474,213 - SO0445 0.43 -1.1 474,217 + SO0445 0.43 -0.1 474,268 - SO0445 0.49 +1.0 474,279 - SO0445 0.50 +1.6 474,427 - SO0445 0.67 +0.6 474,488 + SO0445 0.74 +3.4 474,488 - SO0445 0.74 +0.6 474,488 - SO0445 0.74 +1.1 474,606 + SO0445 0.87 +0.6 474,614 - SO0445 0.88 +0.8 474,665 + +0.1
Or see this region's nucleotide sequence