Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyS and glyQ are separated by 9 nucleotides glyQ and tag are separated by 117 nucleotides tag and SO0017 are separated by 86 nucleotides
SO0014: glyS - glycyl-tRNA synthetase, beta subunit (NCBI ptt file), at 15,180 to 17,246
glyS
SO0015: glyQ - glycyl-tRNA synthetase, alpha subunit (NCBI ptt file), at 17,256 to 18,161
glyQ
SO0016: tag - DNA-3-methyladenine glycosidase I (NCBI ptt file), at 18,279 to 18,854
tag
SO0017: SO0017 - hypothetical amidohydrolase (NCBI ptt file), at 18,941 to 22,090
SO0017
Position (kb) 17
18
19 Strain fitness (log2 ratio) -1
0
1
2 at 18.212 kb on - strand at 18.270 kb on - strand at 18.386 kb on + strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.397 kb on - strand, within tag at 18.399 kb on - strand, within tag at 18.411 kb on + strand, within tag at 18.415 kb on - strand, within tag at 18.419 kb on - strand, within tag at 18.488 kb on - strand, within tag at 18.508 kb on + strand, within tag at 18.511 kb on - strand, within tag at 18.518 kb on + strand, within tag at 18.560 kb on + strand, within tag at 18.587 kb on + strand, within tag at 18.602 kb on + strand, within tag at 18.686 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.775 kb on + strand, within tag at 18.775 kb on + strand, within tag at 18.893 kb on + strand at 18.901 kb on - strand at 18.901 kb on - strand at 18.939 kb on + strand at 18.956 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.971 kb on - strand at 19.016 kb on - strand at 19.016 kb on - strand at 19.034 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 18,212 - -0.1 18,270 - -0.5 18,386 + tag SO0016 0.19 +0.7 18,394 - tag SO0016 0.20 -0.1 18,394 - tag SO0016 0.20 -0.3 18,394 - tag SO0016 0.20 +2.2 18,394 - tag SO0016 0.20 -0.0 18,394 - tag SO0016 0.20 +0.0 18,397 - tag SO0016 0.20 -0.0 18,399 - tag SO0016 0.21 +0.2 18,411 + tag SO0016 0.23 -0.2 18,415 - tag SO0016 0.24 -0.1 18,419 - tag SO0016 0.24 -0.6 18,488 - tag SO0016 0.36 +0.9 18,508 + tag SO0016 0.40 -0.4 18,511 - tag SO0016 0.40 -0.7 18,518 + tag SO0016 0.41 +0.4 18,560 + tag SO0016 0.49 -0.1 18,587 + tag SO0016 0.53 +1.3 18,602 + tag SO0016 0.56 -0.2 18,686 + tag SO0016 0.71 +0.2 18,718 + tag SO0016 0.76 -1.3 18,718 + tag SO0016 0.76 +2.0 18,718 + tag SO0016 0.76 -1.2 18,775 + tag SO0016 0.86 +0.3 18,775 + tag SO0016 0.86 -0.6 18,893 + -0.9 18,901 - +1.0 18,901 - +0.3 18,939 + -1.1 18,956 + -0.3 18,963 + +0.3 18,963 + +0.6 18,963 + +1.4 18,963 + -0.2 18,963 + -0.3 18,971 - +0.3 19,016 - -0.7 19,016 - -1.0 19,034 + -0.5
Or see this region's nucleotide sequence