Experiment: Glycerol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04700 and PGA1_c04710 are separated by 67 nucleotides PGA1_c04710 and PGA1_c04720 are separated by 205 nucleotides PGA1_c04720 and PGA1_c04730 are separated by 11 nucleotides
PGA1_c04700: PGA1_c04700 - hypothetical protein, at 462,069 to 463,013
_c04700
PGA1_c04710: PGA1_c04710 - (dimethylallyl)adenosine tRNA methylthiotransferase MiaB, at 463,081 to 464,403
_c04710
PGA1_c04720: PGA1_c04720 - outer membrane protein OmpA-domain containing protein, at 464,609 to 465,160
_c04720
PGA1_c04730: PGA1_c04730 - hypothetical protein, at 465,172 to 465,474
_c04730
Position (kb)
463
464
465 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 462.085 kb on + strand at 462.085 kb on + strand at 462.093 kb on - strand at 462.139 kb on + strand at 462.164 kb on - strand, within PGA1_c04700 at 462.197 kb on + strand, within PGA1_c04700 at 462.197 kb on + strand, within PGA1_c04700 at 462.213 kb on - strand, within PGA1_c04700 at 462.237 kb on - strand, within PGA1_c04700 at 462.252 kb on + strand, within PGA1_c04700 at 462.260 kb on - strand, within PGA1_c04700 at 462.260 kb on - strand, within PGA1_c04700 at 462.261 kb on + strand, within PGA1_c04700 at 462.267 kb on - strand, within PGA1_c04700 at 462.269 kb on - strand, within PGA1_c04700 at 462.272 kb on - strand, within PGA1_c04700 at 462.272 kb on - strand, within PGA1_c04700 at 462.274 kb on - strand, within PGA1_c04700 at 462.274 kb on - strand, within PGA1_c04700 at 462.275 kb on + strand, within PGA1_c04700 at 462.305 kb on + strand, within PGA1_c04700 at 462.481 kb on - strand, within PGA1_c04700 at 462.543 kb on + strand, within PGA1_c04700 at 462.551 kb on - strand, within PGA1_c04700 at 462.561 kb on + strand, within PGA1_c04700 at 462.603 kb on - strand, within PGA1_c04700 at 462.605 kb on - strand, within PGA1_c04700 at 462.651 kb on - strand, within PGA1_c04700 at 462.651 kb on - strand, within PGA1_c04700 at 462.651 kb on - strand, within PGA1_c04700 at 462.653 kb on + strand, within PGA1_c04700 at 462.763 kb on - strand, within PGA1_c04700 at 462.789 kb on - strand, within PGA1_c04700 at 462.789 kb on - strand, within PGA1_c04700 at 462.812 kb on + strand, within PGA1_c04700 at 462.815 kb on - strand, within PGA1_c04700 at 462.824 kb on + strand, within PGA1_c04700 at 462.824 kb on + strand, within PGA1_c04700 at 462.830 kb on + strand, within PGA1_c04700 at 462.832 kb on - strand, within PGA1_c04700 at 462.843 kb on - strand, within PGA1_c04700 at 462.847 kb on + strand, within PGA1_c04700 at 462.855 kb on - strand, within PGA1_c04700 at 462.872 kb on - strand, within PGA1_c04700 at 462.920 kb on + strand at 462.941 kb on - strand at 462.969 kb on - strand at 463.000 kb on + strand at 463.027 kb on + strand at 463.075 kb on + strand at 463.110 kb on + strand at 463.118 kb on - strand at 463.146 kb on + strand at 463.168 kb on + strand at 463.176 kb on - strand at 463.218 kb on + strand, within PGA1_c04710 at 463.226 kb on + strand, within PGA1_c04710 at 463.227 kb on - strand, within PGA1_c04710 at 463.270 kb on - strand, within PGA1_c04710 at 463.270 kb on - strand, within PGA1_c04710 at 463.275 kb on - strand, within PGA1_c04710 at 463.293 kb on - strand, within PGA1_c04710 at 463.331 kb on + strand, within PGA1_c04710 at 463.331 kb on + strand, within PGA1_c04710 at 463.445 kb on + strand, within PGA1_c04710 at 463.452 kb on - strand, within PGA1_c04710 at 463.453 kb on + strand, within PGA1_c04710 at 463.453 kb on - strand, within PGA1_c04710 at 463.453 kb on - strand, within PGA1_c04710 at 463.453 kb on - strand, within PGA1_c04710 at 463.453 kb on - strand, within PGA1_c04710 at 463.453 kb on - strand, within PGA1_c04710 at 463.520 kb on - strand, within PGA1_c04710 at 463.520 kb on - strand, within PGA1_c04710 at 463.523 kb on + strand, within PGA1_c04710 at 463.529 kb on - strand, within PGA1_c04710 at 463.584 kb on + strand, within PGA1_c04710 at 463.592 kb on - strand, within PGA1_c04710 at 463.639 kb on + strand, within PGA1_c04710 at 463.639 kb on + strand, within PGA1_c04710 at 463.655 kb on + strand, within PGA1_c04710 at 463.655 kb on + strand, within PGA1_c04710 at 463.733 kb on + strand, within PGA1_c04710 at 463.780 kb on - strand, within PGA1_c04710 at 463.837 kb on + strand, within PGA1_c04710 at 463.872 kb on - strand, within PGA1_c04710 at 463.874 kb on + strand, within PGA1_c04710 at 463.884 kb on + strand, within PGA1_c04710 at 463.892 kb on - strand, within PGA1_c04710 at 463.916 kb on + strand, within PGA1_c04710 at 463.917 kb on - strand, within PGA1_c04710 at 464.045 kb on + strand, within PGA1_c04710 at 464.052 kb on - strand, within PGA1_c04710 at 464.053 kb on - strand, within PGA1_c04710 at 464.053 kb on - strand, within PGA1_c04710 at 464.107 kb on - strand, within PGA1_c04710 at 464.206 kb on + strand, within PGA1_c04710 at 464.214 kb on - strand, within PGA1_c04710 at 464.214 kb on - strand, within PGA1_c04710 at 464.216 kb on + strand, within PGA1_c04710 at 464.224 kb on - strand, within PGA1_c04710 at 464.286 kb on - strand at 464.294 kb on + strand at 464.355 kb on + strand at 464.530 kb on - strand at 464.530 kb on - strand at 464.530 kb on - strand at 464.533 kb on + strand at 464.533 kb on + strand at 464.541 kb on - strand at 464.541 kb on - strand at 464.541 kb on - strand at 464.556 kb on + strand at 464.577 kb on + strand at 464.577 kb on + strand at 464.579 kb on - strand at 464.584 kb on - strand at 464.599 kb on + strand at 464.607 kb on - strand at 464.630 kb on + strand at 464.630 kb on + strand at 464.630 kb on + strand at 464.630 kb on + strand at 464.638 kb on - strand at 464.638 kb on - strand at 464.638 kb on - strand at 464.666 kb on - strand, within PGA1_c04720 at 464.666 kb on - strand, within PGA1_c04720 at 464.688 kb on + strand, within PGA1_c04720 at 464.696 kb on - strand, within PGA1_c04720 at 464.717 kb on - strand, within PGA1_c04720 at 464.730 kb on - strand, within PGA1_c04720 at 464.730 kb on - strand, within PGA1_c04720 at 464.757 kb on - strand, within PGA1_c04720 at 464.778 kb on + strand, within PGA1_c04720 at 464.833 kb on + strand, within PGA1_c04720 at 464.841 kb on + strand, within PGA1_c04720 at 464.868 kb on - strand, within PGA1_c04720 at 464.897 kb on + strand, within PGA1_c04720 at 464.905 kb on - strand, within PGA1_c04720 at 464.905 kb on - strand, within PGA1_c04720 at 464.905 kb on - strand, within PGA1_c04720 at 464.905 kb on - strand, within PGA1_c04720 at 464.905 kb on - strand, within PGA1_c04720 at 464.976 kb on + strand, within PGA1_c04720 at 464.984 kb on - strand, within PGA1_c04720 at 465.025 kb on + strand, within PGA1_c04720 at 465.057 kb on + strand, within PGA1_c04720 at 465.065 kb on - strand, within PGA1_c04720 at 465.070 kb on + strand, within PGA1_c04720 at 465.078 kb on - strand, within PGA1_c04720 at 465.130 kb on - strand at 465.154 kb on + strand at 465.162 kb on - strand at 465.162 kb on - strand at 465.164 kb on + strand at 465.195 kb on + strand at 465.201 kb on - strand at 465.267 kb on + strand, within PGA1_c04730 at 465.282 kb on - strand, within PGA1_c04730 at 465.287 kb on - strand, within PGA1_c04730 at 465.350 kb on + strand, within PGA1_c04730 at 465.401 kb on + strand, within PGA1_c04730
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol (C) remove 462,085 + +1.6 462,085 + -2.0 462,093 - -3.1 462,139 + +2.0 462,164 - PGA1_c04700 0.10 -1.4 462,197 + PGA1_c04700 0.14 +1.6 462,197 + PGA1_c04700 0.14 +3.2 462,213 - PGA1_c04700 0.15 -0.6 462,237 - PGA1_c04700 0.18 +0.4 462,252 + PGA1_c04700 0.19 +1.3 462,260 - PGA1_c04700 0.20 -1.0 462,260 - PGA1_c04700 0.20 -1.2 462,261 + PGA1_c04700 0.20 +0.2 462,267 - PGA1_c04700 0.21 -0.7 462,269 - PGA1_c04700 0.21 +0.1 462,272 - PGA1_c04700 0.21 -0.4 462,272 - PGA1_c04700 0.21 +2.6 462,274 - PGA1_c04700 0.22 -0.3 462,274 - PGA1_c04700 0.22 -0.1 462,275 + PGA1_c04700 0.22 -1.0 462,305 + PGA1_c04700 0.25 -0.4 462,481 - PGA1_c04700 0.44 -0.0 462,543 + PGA1_c04700 0.50 -1.1 462,551 - PGA1_c04700 0.51 +2.2 462,561 + PGA1_c04700 0.52 -0.0 462,603 - PGA1_c04700 0.57 -0.6 462,605 - PGA1_c04700 0.57 +2.2 462,651 - PGA1_c04700 0.62 -0.1 462,651 - PGA1_c04700 0.62 +1.2 462,651 - PGA1_c04700 0.62 +0.2 462,653 + PGA1_c04700 0.62 -1.9 462,763 - PGA1_c04700 0.73 +1.1 462,789 - PGA1_c04700 0.76 +0.3 462,789 - PGA1_c04700 0.76 +0.8 462,812 + PGA1_c04700 0.79 +0.6 462,815 - PGA1_c04700 0.79 +0.1 462,824 + PGA1_c04700 0.80 +0.4 462,824 + PGA1_c04700 0.80 +0.4 462,830 + PGA1_c04700 0.81 -0.0 462,832 - PGA1_c04700 0.81 +0.6 462,843 - PGA1_c04700 0.82 +1.1 462,847 + PGA1_c04700 0.82 +1.0 462,855 - PGA1_c04700 0.83 +0.4 462,872 - PGA1_c04700 0.85 +0.3 462,920 + +2.2 462,941 - +1.0 462,969 - +0.7 463,000 + -2.1 463,027 + +0.6 463,075 + -0.1 463,110 + -0.0 463,118 - +1.2 463,146 + +0.6 463,168 + -1.9 463,176 - +0.8 463,218 + PGA1_c04710 0.10 +0.7 463,226 + PGA1_c04710 0.11 -0.2 463,227 - PGA1_c04710 0.11 -0.7 463,270 - PGA1_c04710 0.14 +0.6 463,270 - PGA1_c04710 0.14 -2.2 463,275 - PGA1_c04710 0.15 +0.5 463,293 - PGA1_c04710 0.16 +0.3 463,331 + PGA1_c04710 0.19 +1.7 463,331 + PGA1_c04710 0.19 -1.4 463,445 + PGA1_c04710 0.28 +0.2 463,452 - PGA1_c04710 0.28 +0.9 463,453 + PGA1_c04710 0.28 -1.2 463,453 - PGA1_c04710 0.28 +0.1 463,453 - PGA1_c04710 0.28 +1.1 463,453 - PGA1_c04710 0.28 -3.8 463,453 - PGA1_c04710 0.28 -1.2 463,453 - PGA1_c04710 0.28 -0.0 463,520 - PGA1_c04710 0.33 +0.4 463,520 - PGA1_c04710 0.33 +2.2 463,523 + PGA1_c04710 0.33 +0.2 463,529 - PGA1_c04710 0.34 +0.6 463,584 + PGA1_c04710 0.38 +0.8 463,592 - PGA1_c04710 0.39 -0.3 463,639 + PGA1_c04710 0.42 -1.0 463,639 + PGA1_c04710 0.42 +0.1 463,655 + PGA1_c04710 0.43 +0.5 463,655 + PGA1_c04710 0.43 +1.5 463,733 + PGA1_c04710 0.49 -0.4 463,780 - PGA1_c04710 0.53 +1.1 463,837 + PGA1_c04710 0.57 -3.0 463,872 - PGA1_c04710 0.60 -3.5 463,874 + PGA1_c04710 0.60 +0.7 463,884 + PGA1_c04710 0.61 -2.0 463,892 - PGA1_c04710 0.61 +1.6 463,916 + PGA1_c04710 0.63 +1.3 463,917 - PGA1_c04710 0.63 +0.8 464,045 + PGA1_c04710 0.73 -0.0 464,052 - PGA1_c04710 0.73 -3.0 464,053 - PGA1_c04710 0.73 -1.4 464,053 - PGA1_c04710 0.73 +1.2 464,107 - PGA1_c04710 0.78 +0.9 464,206 + PGA1_c04710 0.85 -2.4 464,214 - PGA1_c04710 0.86 -0.1 464,214 - PGA1_c04710 0.86 -0.0 464,216 + PGA1_c04710 0.86 +1.1 464,224 - PGA1_c04710 0.86 -2.5 464,286 - -2.3 464,294 + -0.1 464,355 + -0.2 464,530 - -0.0 464,530 - +0.6 464,530 - +0.9 464,533 + -0.1 464,533 + -1.2 464,541 - -2.4 464,541 - -0.9 464,541 - -2.8 464,556 + -1.2 464,577 + +0.4 464,577 + -1.2 464,579 - +1.4 464,584 - -0.8 464,599 + -0.6 464,607 - +1.9 464,630 + +0.1 464,630 + +0.4 464,630 + -1.8 464,630 + +1.8 464,638 - +0.8 464,638 - +0.7 464,638 - -1.4 464,666 - PGA1_c04720 0.10 +0.4 464,666 - PGA1_c04720 0.10 +0.8 464,688 + PGA1_c04720 0.14 +0.4 464,696 - PGA1_c04720 0.16 +0.7 464,717 - PGA1_c04720 0.20 +0.2 464,730 - PGA1_c04720 0.22 -1.8 464,730 - PGA1_c04720 0.22 -0.1 464,757 - PGA1_c04720 0.27 -2.0 464,778 + PGA1_c04720 0.31 -1.3 464,833 + PGA1_c04720 0.41 +0.6 464,841 + PGA1_c04720 0.42 -2.4 464,868 - PGA1_c04720 0.47 -0.4 464,897 + PGA1_c04720 0.52 -3.5 464,905 - PGA1_c04720 0.54 +0.2 464,905 - PGA1_c04720 0.54 -1.2 464,905 - PGA1_c04720 0.54 -2.0 464,905 - PGA1_c04720 0.54 -0.8 464,905 - PGA1_c04720 0.54 -0.2 464,976 + PGA1_c04720 0.66 +0.3 464,984 - PGA1_c04720 0.68 +0.3 465,025 + PGA1_c04720 0.75 -1.1 465,057 + PGA1_c04720 0.81 +1.6 465,065 - PGA1_c04720 0.83 +1.0 465,070 + PGA1_c04720 0.84 +0.5 465,078 - PGA1_c04720 0.85 -1.1 465,130 - -2.1 465,154 + -2.8 465,162 - +1.7 465,162 - +0.4 465,164 + +1.1 465,195 + -1.5 465,201 - -0.9 465,267 + PGA1_c04730 0.31 +0.3 465,282 - PGA1_c04730 0.36 +1.2 465,287 - PGA1_c04730 0.38 -1.0 465,350 + PGA1_c04730 0.59 -0.0 465,401 + PGA1_c04730 0.76 -0.6
Or see this region's nucleotide sequence