Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2469 and SO2470 are separated by 295 nucleotides SO2470 and dapE overlap by 4 nucleotides dapE and SO2472 are separated by 5 nucleotides SO2472 and SO2473 are separated by 69 nucleotides
SO2469: SO2469 - hypothetical TonB-dependent receptor (NCBI ptt file), at 2,589,746 to 2,592,421
SO2469
SO2470: SO2470 - conserved hypothetical protein (NCBI ptt file), at 2,592,717 to 2,593,061
SO2470
SO2471: dapE - succinyl-diaminopimelate desuccinylase (NCBI ptt file), at 2,593,058 to 2,594,212
dapE
SO2472: SO2472 - D-alanyl-D-alanine carboxypeptidase-related protein (NCBI ptt file), at 2,594,218 to 2,594,976
SO2472
SO2473: SO2473 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,595,046 to 2,595,879
SO2473
Position (kb)
2593
2594
2595 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2592.061 kb on - strand, within SO2469 at 2592.068 kb on - strand, within SO2469 at 2592.078 kb on + strand, within SO2469 at 2592.157 kb on - strand at 2592.162 kb on - strand at 2592.173 kb on + strand at 2592.181 kb on - strand at 2592.217 kb on - strand at 2592.233 kb on + strand at 2592.241 kb on + strand at 2592.260 kb on + strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.279 kb on + strand at 2592.318 kb on - strand at 2592.399 kb on - strand at 2592.551 kb on + strand at 2592.615 kb on + strand at 2592.825 kb on + strand, within SO2470 at 2592.938 kb on + strand, within SO2470 at 2594.244 kb on + strand at 2594.257 kb on + strand at 2594.257 kb on + strand at 2594.342 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.970 kb on + strand at 2595.096 kb on + strand at 2595.146 kb on + strand, within SO2473 at 2595.148 kb on + strand, within SO2473
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,592,061 - SO2469 0.87 -0.1 2,592,068 - SO2469 0.87 -0.0 2,592,078 + SO2469 0.87 +1.6 2,592,157 - -0.5 2,592,162 - +1.2 2,592,173 + -0.6 2,592,181 - +0.0 2,592,217 - +1.0 2,592,233 + -0.2 2,592,241 + -0.9 2,592,260 + +0.4 2,592,268 - +0.5 2,592,268 - -0.8 2,592,268 - +0.2 2,592,268 - -0.2 2,592,268 - -0.1 2,592,279 + +0.2 2,592,318 - -0.4 2,592,399 - +0.1 2,592,551 + +0.0 2,592,615 + -2.4 2,592,825 + SO2470 0.31 -0.6 2,592,938 + SO2470 0.64 +0.9 2,594,244 + +0.3 2,594,257 + -2.2 2,594,257 + -3.7 2,594,342 + SO2472 0.16 -3.0 2,594,801 + SO2472 0.77 -2.7 2,594,801 + SO2472 0.77 -3.2 2,594,970 + +0.8 2,595,096 + -0.2 2,595,146 + SO2473 0.12 +0.1 2,595,148 + SO2473 0.12 +0.6
Or see this region's nucleotide sequence