Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt suhB and SO2261 are separated by 154 nucleotides SO2261 and cysE are separated by 46 nucleotides cysE and SO2263 are separated by 353 nucleotides SO2263 and iscS are separated by 78 nucleotides
SO2260: suhB - extragenic suppressor protein SuhB (NCBI ptt file), at 2,375,506 to 2,376,309
suhB
SO2261: SO2261 - RNA methyltransferase, TrmH family, group 1 (NCBI ptt file), at 2,376,464 to 2,377,189
SO2261
SO2262: cysE - serine acetyltransferase (NCBI ptt file), at 2,377,236 to 2,378,057
cysE
SO2263: SO2263 - Rrf2 family protein (NCBI ptt file), at 2,378,411 to 2,378,872
SO2263
SO2264: iscS - cysteine desulfurase (NCBI ptt file), at 2,378,951 to 2,380,165
iscS
Position (kb)
2377
2378
2379 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2376.352 kb on + strand at 2376.490 kb on + strand at 2376.552 kb on + strand, within SO2261 at 2376.643 kb on + strand, within SO2261 at 2376.657 kb on + strand, within SO2261 at 2376.657 kb on + strand, within SO2261 at 2376.724 kb on - strand, within SO2261 at 2376.886 kb on - strand, within SO2261 at 2376.886 kb on - strand, within SO2261 at 2376.887 kb on + strand, within SO2261 at 2377.016 kb on + strand, within SO2261 at 2377.067 kb on + strand, within SO2261 at 2377.067 kb on + strand, within SO2261 at 2377.174 kb on - strand at 2377.195 kb on + strand at 2377.226 kb on + strand at 2378.046 kb on - strand at 2378.168 kb on - strand at 2378.172 kb on + strand at 2378.316 kb on + strand at 2378.657 kb on + strand, within SO2263 at 2378.657 kb on + strand, within SO2263 at 2378.657 kb on + strand, within SO2263 at 2378.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,376,352 + -0.3 2,376,490 + -1.2 2,376,552 + SO2261 0.12 -0.0 2,376,643 + SO2261 0.25 -0.4 2,376,657 + SO2261 0.27 -0.8 2,376,657 + SO2261 0.27 +0.1 2,376,724 - SO2261 0.36 -2.7 2,376,886 - SO2261 0.58 -1.4 2,376,886 - SO2261 0.58 -3.0 2,376,887 + SO2261 0.58 +0.9 2,377,016 + SO2261 0.76 +0.6 2,377,067 + SO2261 0.83 +0.1 2,377,067 + SO2261 0.83 +1.8 2,377,174 - -1.4 2,377,195 + -0.1 2,377,226 + +0.4 2,378,046 - -1.7 2,378,168 - +1.9 2,378,172 + -0.5 2,378,316 + -0.8 2,378,657 + SO2263 0.53 +0.2 2,378,657 + SO2263 0.53 +1.2 2,378,657 + SO2263 0.53 -0.2 2,378,843 + -0.3
Or see this region's nucleotide sequence