Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 63 nucleotides rbfA and SO_1206 overlap by 1 nucleotides SO_1206 and rpsO are separated by 127 nucleotides rpsO and SO1208 are separated by 91 nucleotides
SO1204: infB - translation initiation factor IF-2 (NCBI ptt file), at 1,247,966 to 1,250,623
infB
SO1205: rbfA - ribosome-binding factor A (NCBI ptt file), at 1,250,687 to 1,251,130
rbfA
SO_1206: SO_1206 - no description, at 1,251,130 to 1,252,083
_1206
SO1207: rpsO - ribosomal protein S15 (NCBI ptt file), at 1,252,211 to 1,252,480
rpsO
SO1208: SO1208 - GGDEF domain protein (NCBI ptt file), at 1,252,572 to 1,254,626
SO1208
Position (kb)
1251
1252
1253 Strain fitness (log2 ratio)
-2
-1
0
1 at 1251.028 kb on + strand, within rbfA at 1251.125 kb on + strand at 1251.250 kb on + strand, within SO_1206 at 1251.404 kb on + strand, within SO_1206 at 1251.656 kb on - strand, within SO_1206 at 1251.765 kb on + strand, within SO_1206 at 1251.770 kb on + strand, within SO_1206 at 1251.770 kb on + strand, within SO_1206 at 1251.770 kb on + strand, within SO_1206 at 1251.770 kb on + strand, within SO_1206 at 1251.770 kb on + strand, within SO_1206 at 1251.770 kb on + strand, within SO_1206 at 1251.778 kb on - strand, within SO_1206 at 1251.778 kb on - strand, within SO_1206 at 1251.778 kb on - strand, within SO_1206 at 1251.778 kb on - strand, within SO_1206 at 1251.778 kb on - strand, within SO_1206 at 1251.778 kb on - strand, within SO_1206 at 1251.900 kb on + strand, within SO_1206 at 1251.908 kb on - strand, within SO_1206 at 1251.983 kb on + strand, within SO_1206 at 1252.068 kb on + strand at 1252.076 kb on - strand at 1252.076 kb on - strand at 1252.629 kb on - strand at 1252.699 kb on + strand at 1252.808 kb on - strand, within SO1208 at 1252.848 kb on + strand, within SO1208 at 1252.932 kb on - strand, within SO1208 at 1252.952 kb on - strand, within SO1208 at 1253.080 kb on - strand, within SO1208
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 1,251,028 + rbfA SO1205 0.77 -0.3 1,251,125 + +0.1 1,251,250 + SO_1206 0.13 -1.6 1,251,404 + SO_1206 0.29 -1.3 1,251,656 - SO_1206 0.55 -0.5 1,251,765 + SO_1206 0.67 -1.7 1,251,770 + SO_1206 0.67 -2.6 1,251,770 + SO_1206 0.67 -0.2 1,251,770 + SO_1206 0.67 -0.7 1,251,770 + SO_1206 0.67 -1.4 1,251,770 + SO_1206 0.67 +0.1 1,251,770 + SO_1206 0.67 -1.5 1,251,778 - SO_1206 0.68 -0.9 1,251,778 - SO_1206 0.68 +0.3 1,251,778 - SO_1206 0.68 -0.4 1,251,778 - SO_1206 0.68 -0.9 1,251,778 - SO_1206 0.68 -0.6 1,251,778 - SO_1206 0.68 +0.2 1,251,900 + SO_1206 0.81 -0.9 1,251,908 - SO_1206 0.82 -0.1 1,251,983 + SO_1206 0.89 -0.8 1,252,068 + -0.9 1,252,076 - -0.4 1,252,076 - +0.8 1,252,629 - +1.0 1,252,699 + -0.7 1,252,808 - SO1208 0.11 -1.1 1,252,848 + SO1208 0.13 +0.4 1,252,932 - SO1208 0.18 -0.2 1,252,952 - SO1208 0.18 +0.1 1,253,080 - SO1208 0.25 +1.2
Or see this region's nucleotide sequence