Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and Sama_1857 are separated by 1 nucleotides Sama_1857 and Sama_1858 overlap by 7 nucleotides Sama_1858 and Sama_1859 overlap by 11 nucleotides
Sama_1855: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,259,305 to 2,260,420
thiH
Sama_1856: thiG - thiazole synthase (RefSeq), at 2,260,417 to 2,261,232
thiG
Sama_1857: Sama_1857 - thiamine biosynthesis protein ThiS (RefSeq), at 2,261,234 to 2,261,437
_1857
Sama_1858: Sama_1858 - thiF protein, putative (RefSeq), at 2,261,431 to 2,262,183
_1858
Sama_1859: Sama_1859 - phosphomethylpyrimidine kinase/thiamine-phosphate diphosphorylase (RefSeq), at 2,262,173 to 2,263,750
_1859
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2259.432 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.587 kb on - strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.684 kb on + strand, within thiH at 2259.793 kb on + strand, within thiH at 2259.809 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.862 kb on - strand, within thiH at 2259.955 kb on - strand, within thiH at 2259.988 kb on + strand, within thiH at 2260.051 kb on - strand, within thiH at 2260.085 kb on - strand, within thiH at 2260.115 kb on - strand, within thiH at 2260.143 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.193 kb on - strand, within thiH at 2260.217 kb on - strand, within thiH at 2260.257 kb on + strand, within thiH at 2260.353 kb on + strand at 2260.372 kb on + strand at 2260.372 kb on + strand at 2260.372 kb on + strand at 2260.408 kb on - strand at 2260.474 kb on - strand at 2260.621 kb on - strand, within thiG at 2260.623 kb on + strand, within thiG at 2260.768 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.855 kb on + strand, within thiG at 2260.856 kb on - strand, within thiG at 2260.856 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.922 kb on + strand, within thiG at 2260.923 kb on - strand, within thiG at 2261.164 kb on - strand at 2261.281 kb on + strand, within Sama_1857 at 2261.290 kb on + strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.302 kb on + strand, within Sama_1857 at 2261.303 kb on - strand, within Sama_1857 at 2261.315 kb on + strand, within Sama_1857 at 2261.315 kb on + strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.369 kb on - strand, within Sama_1857 at 2261.428 kb on + strand at 2261.623 kb on - strand, within Sama_1858 at 2261.623 kb on - strand, within Sama_1858 at 2261.748 kb on + strand, within Sama_1858 at 2261.749 kb on - strand, within Sama_1858 at 2261.775 kb on - strand, within Sama_1858 at 2261.784 kb on + strand, within Sama_1858 at 2261.848 kb on + strand, within Sama_1858 at 2261.849 kb on - strand, within Sama_1858 at 2261.862 kb on + strand, within Sama_1858 at 2261.863 kb on - strand, within Sama_1858 at 2261.874 kb on - strand, within Sama_1858 at 2261.888 kb on - strand, within Sama_1858 at 2261.888 kb on - strand, within Sama_1858 at 2261.983 kb on - strand, within Sama_1858 at 2262.016 kb on + strand, within Sama_1858 at 2262.016 kb on + strand, within Sama_1858 at 2262.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 2,259,432 + thiH Sama_1855 0.11 -0.4 2,259,448 + thiH Sama_1855 0.13 +0.1 2,259,448 + thiH Sama_1855 0.13 +1.3 2,259,448 + thiH Sama_1855 0.13 -0.5 2,259,448 + thiH Sama_1855 0.13 +0.1 2,259,448 + thiH Sama_1855 0.13 -0.9 2,259,449 - thiH Sama_1855 0.13 -0.4 2,259,449 - thiH Sama_1855 0.13 +2.2 2,259,449 - thiH Sama_1855 0.13 +0.1 2,259,449 - thiH Sama_1855 0.13 +0.4 2,259,449 - thiH Sama_1855 0.13 -0.9 2,259,568 + thiH Sama_1855 0.24 -1.5 2,259,568 + thiH Sama_1855 0.24 -0.4 2,259,569 - thiH Sama_1855 0.24 +0.5 2,259,569 - thiH Sama_1855 0.24 -0.8 2,259,569 - thiH Sama_1855 0.24 +0.8 2,259,569 - thiH Sama_1855 0.24 +0.2 2,259,569 - thiH Sama_1855 0.24 +2.9 2,259,587 - thiH Sama_1855 0.25 +0.4 2,259,649 + thiH Sama_1855 0.31 +0.1 2,259,649 + thiH Sama_1855 0.31 +1.7 2,259,684 + thiH Sama_1855 0.34 +1.3 2,259,793 + thiH Sama_1855 0.44 +0.7 2,259,809 - thiH Sama_1855 0.45 +1.3 2,259,847 - thiH Sama_1855 0.49 -0.2 2,259,847 - thiH Sama_1855 0.49 -0.5 2,259,847 - thiH Sama_1855 0.49 -3.1 2,259,847 - thiH Sama_1855 0.49 +0.6 2,259,862 - thiH Sama_1855 0.50 -1.1 2,259,955 - thiH Sama_1855 0.58 +0.6 2,259,988 + thiH Sama_1855 0.61 -1.1 2,260,051 - thiH Sama_1855 0.67 -0.5 2,260,085 - thiH Sama_1855 0.70 +0.2 2,260,115 - thiH Sama_1855 0.73 +0.6 2,260,143 + thiH Sama_1855 0.75 -1.8 2,260,176 + thiH Sama_1855 0.78 +0.6 2,260,176 + thiH Sama_1855 0.78 -0.5 2,260,176 + thiH Sama_1855 0.78 +0.4 2,260,177 - thiH Sama_1855 0.78 -0.3 2,260,177 - thiH Sama_1855 0.78 +0.5 2,260,188 + thiH Sama_1855 0.79 -0.1 2,260,188 + thiH Sama_1855 0.79 -0.1 2,260,188 + thiH Sama_1855 0.79 -0.9 2,260,189 - thiH Sama_1855 0.79 +0.9 2,260,189 - thiH Sama_1855 0.79 +1.8 2,260,192 + thiH Sama_1855 0.79 +0.8 2,260,192 + thiH Sama_1855 0.79 -0.1 2,260,192 + thiH Sama_1855 0.79 +1.5 2,260,193 - thiH Sama_1855 0.80 -0.5 2,260,217 - thiH Sama_1855 0.82 +3.2 2,260,257 + thiH Sama_1855 0.85 +0.8 2,260,353 + -0.7 2,260,372 + +2.2 2,260,372 + -0.6 2,260,372 + +1.8 2,260,408 - -0.4 2,260,474 - +0.8 2,260,621 - thiG Sama_1856 0.25 -2.5 2,260,623 + thiG Sama_1856 0.25 -0.9 2,260,768 + thiG Sama_1856 0.43 +0.7 2,260,847 + thiG Sama_1856 0.53 -1.2 2,260,847 + thiG Sama_1856 0.53 -0.3 2,260,847 + thiG Sama_1856 0.53 -1.6 2,260,848 - thiG Sama_1856 0.53 -0.0 2,260,848 - thiG Sama_1856 0.53 +3.1 2,260,848 - thiG Sama_1856 0.53 +0.5 2,260,848 - thiG Sama_1856 0.53 -0.6 2,260,848 - thiG Sama_1856 0.53 +0.2 2,260,848 - thiG Sama_1856 0.53 +0.1 2,260,848 - thiG Sama_1856 0.53 +2.5 2,260,848 - thiG Sama_1856 0.53 +1.9 2,260,855 + thiG Sama_1856 0.54 +0.0 2,260,856 - thiG Sama_1856 0.54 -0.2 2,260,856 - thiG Sama_1856 0.54 -0.4 2,260,861 - thiG Sama_1856 0.54 +1.5 2,260,861 - thiG Sama_1856 0.54 +0.3 2,260,861 - thiG Sama_1856 0.54 +0.2 2,260,922 + thiG Sama_1856 0.62 +0.5 2,260,923 - thiG Sama_1856 0.62 -0.3 2,261,164 - -0.1 2,261,281 + Sama_1857 0.23 +0.1 2,261,290 + Sama_1857 0.27 +0.0 2,261,291 - Sama_1857 0.28 -0.8 2,261,291 - Sama_1857 0.28 +0.9 2,261,291 - Sama_1857 0.28 +0.4 2,261,291 - Sama_1857 0.28 +0.5 2,261,302 + Sama_1857 0.33 -0.5 2,261,303 - Sama_1857 0.34 -0.4 2,261,315 + Sama_1857 0.40 +0.5 2,261,315 + Sama_1857 0.40 -0.4 2,261,316 - Sama_1857 0.40 +0.5 2,261,316 - Sama_1857 0.40 -0.0 2,261,316 - Sama_1857 0.40 +1.1 2,261,369 - Sama_1857 0.66 +0.0 2,261,428 + -0.3 2,261,623 - Sama_1858 0.25 +1.0 2,261,623 - Sama_1858 0.25 -0.5 2,261,748 + Sama_1858 0.42 -0.4 2,261,749 - Sama_1858 0.42 -2.2 2,261,775 - Sama_1858 0.46 -0.3 2,261,784 + Sama_1858 0.47 -1.2 2,261,848 + Sama_1858 0.55 -0.7 2,261,849 - Sama_1858 0.56 -0.3 2,261,862 + Sama_1858 0.57 +0.7 2,261,863 - Sama_1858 0.57 +0.1 2,261,874 - Sama_1858 0.59 -0.8 2,261,888 - Sama_1858 0.61 +1.0 2,261,888 - Sama_1858 0.61 -0.2 2,261,983 - Sama_1858 0.73 +0.4 2,262,016 + Sama_1858 0.78 -0.1 2,262,016 + Sama_1858 0.78 -0.4 2,262,163 - -0.3
Or see this region's nucleotide sequence