Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0170 and Sama_0171 are separated by 3 nucleotides Sama_0171 and Sama_0172 are separated by 37 nucleotides Sama_0172 and Sama_0173 are separated by 12 nucleotides
Sama_0170: Sama_0170 - general secretion pathway protein E (RefSeq), at 200,369 to 201,931
_0170
Sama_0171: Sama_0171 - general secretion pathway protein F (RefSeq), at 201,935 to 203,158
_0171
Sama_0172: Sama_0172 - general secretion pathway protein G (RefSeq), at 203,196 to 203,627
_0172
Sama_0173: Sama_0173 - general secretion pathway protein H (RefSeq), at 203,640 to 204,209
_0173
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 201.026 kb on - strand, within Sama_0170 at 201.119 kb on - strand, within Sama_0170 at 201.311 kb on + strand, within Sama_0170 at 201.325 kb on + strand, within Sama_0170 at 201.326 kb on - strand, within Sama_0170 at 201.327 kb on + strand, within Sama_0170 at 201.328 kb on - strand, within Sama_0170 at 201.470 kb on - strand, within Sama_0170 at 201.521 kb on - strand, within Sama_0170 at 201.521 kb on - strand, within Sama_0170 at 201.582 kb on + strand, within Sama_0170 at 201.600 kb on + strand, within Sama_0170 at 201.622 kb on + strand, within Sama_0170 at 201.790 kb on + strand at 201.791 kb on - strand at 201.907 kb on - strand at 201.907 kb on - strand at 201.929 kb on + strand at 202.283 kb on + strand, within Sama_0171 at 202.284 kb on - strand, within Sama_0171 at 202.343 kb on - strand, within Sama_0171 at 202.343 kb on - strand, within Sama_0171 at 202.571 kb on - strand, within Sama_0171 at 202.608 kb on - strand, within Sama_0171 at 202.617 kb on + strand, within Sama_0171 at 202.843 kb on - strand, within Sama_0171 at 202.946 kb on + strand, within Sama_0171 at 202.946 kb on + strand, within Sama_0171 at 202.947 kb on - strand, within Sama_0171 at 202.949 kb on - strand, within Sama_0171 at 203.101 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.160 kb on + strand at 203.160 kb on + strand at 203.160 kb on + strand at 203.178 kb on + strand at 203.178 kb on + strand at 203.178 kb on + strand at 203.179 kb on - strand at 203.419 kb on + strand, within Sama_0172 at 203.453 kb on + strand, within Sama_0172 at 203.512 kb on - strand, within Sama_0172 at 203.571 kb on - strand, within Sama_0172 at 203.594 kb on + strand at 203.620 kb on - strand at 203.625 kb on + strand at 203.625 kb on + strand at 203.625 kb on + strand at 203.626 kb on - strand at 203.665 kb on + strand at 203.687 kb on - strand at 203.849 kb on + strand, within Sama_0173 at 203.850 kb on - strand, within Sama_0173 at 203.853 kb on + strand, within Sama_0173 at 203.854 kb on - strand, within Sama_0173 at 203.859 kb on + strand, within Sama_0173 at 203.860 kb on - strand, within Sama_0173 at 203.868 kb on + strand, within Sama_0173 at 203.942 kb on + strand, within Sama_0173 at 204.015 kb on - strand, within Sama_0173 at 204.070 kb on + strand, within Sama_0173
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 201,026 - Sama_0170 0.42 -1.6 201,119 - Sama_0170 0.48 +1.0 201,311 + Sama_0170 0.60 -1.0 201,325 + Sama_0170 0.61 -0.9 201,326 - Sama_0170 0.61 -1.6 201,327 + Sama_0170 0.61 +2.0 201,328 - Sama_0170 0.61 -0.2 201,470 - Sama_0170 0.70 -1.0 201,521 - Sama_0170 0.74 -0.0 201,521 - Sama_0170 0.74 -1.2 201,582 + Sama_0170 0.78 -2.0 201,600 + Sama_0170 0.79 -1.5 201,622 + Sama_0170 0.80 -1.0 201,790 + -2.1 201,791 - -0.0 201,907 - -1.8 201,907 - +0.4 201,929 + +0.6 202,283 + Sama_0171 0.28 -2.1 202,284 - Sama_0171 0.29 -2.1 202,343 - Sama_0171 0.33 -1.4 202,343 - Sama_0171 0.33 -0.9 202,571 - Sama_0171 0.52 -1.2 202,608 - Sama_0171 0.55 -0.1 202,617 + Sama_0171 0.56 -0.1 202,843 - Sama_0171 0.74 -1.5 202,946 + Sama_0171 0.83 +0.4 202,946 + Sama_0171 0.83 -1.1 202,947 - Sama_0171 0.83 -0.6 202,949 - Sama_0171 0.83 -1.4 203,101 + -1.1 203,156 + -1.2 203,156 + -3.2 203,156 + +1.3 203,156 + -1.3 203,156 + -0.6 203,156 + -1.1 203,160 + +1.4 203,160 + -0.1 203,160 + -0.3 203,178 + -0.3 203,178 + -0.4 203,178 + +1.4 203,179 - -0.4 203,419 + Sama_0172 0.52 -0.6 203,453 + Sama_0172 0.59 -0.4 203,512 - Sama_0172 0.73 -0.9 203,571 - Sama_0172 0.87 -1.4 203,594 + -2.0 203,620 - -0.4 203,625 + +0.2 203,625 + -1.5 203,625 + +0.1 203,626 - -1.2 203,665 + -0.2 203,687 - -0.1 203,849 + Sama_0173 0.37 +2.9 203,850 - Sama_0173 0.37 -1.6 203,853 + Sama_0173 0.37 +3.2 203,854 - Sama_0173 0.38 -0.6 203,859 + Sama_0173 0.38 -0.9 203,860 - Sama_0173 0.39 -0.5 203,868 + Sama_0173 0.40 -0.6 203,942 + Sama_0173 0.53 -0.6 204,015 - Sama_0173 0.66 -0.6 204,070 + Sama_0173 0.75 -0.4
Or see this region's nucleotide sequence