Experiment: propionate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3527 and SO3528 are separated by 33 nucleotides SO3528 and lytB are separated by 217 nucleotides lytB and fkbP-2 are separated by 1 nucleotides fkbP-2 and lspA overlap by 1 nucleotides lspA and ileS are separated by 9 nucleotides
SO3527: SO3527 - hypothetical protein (NCBI ptt file), at 3,682,156 to 3,683,094
SO3527
SO3528: SO3528 - hypothetical protein (NCBI ptt file), at 3,683,128 to 3,683,691
SO3528
SO3529: lytB - penicillin tolerance protein LytB (NCBI ptt file), at 3,683,909 to 3,684,865
lytB
SO3530: fkbP-2 - peptidyl-prolyl cis-trans isomerase FkbP (NCBI ptt file), at 3,684,867 to 3,685,286
fkbP-2
SO3531: lspA - lipoprotein signal peptidase (NCBI ptt file), at 3,685,286 to 3,685,798
lspA
SO3532: ileS - isoleucyl-tRNA synthetase (NCBI ptt file), at 3,685,808 to 3,688,630
ileS
Position (kb)
3683
3684
3685 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3682.928 kb on + strand, within SO3527 at 3682.956 kb on - strand, within SO3527 at 3683.037 kb on + strand at 3683.239 kb on - strand, within SO3528 at 3683.354 kb on - strand, within SO3528 at 3683.392 kb on - strand, within SO3528 at 3683.412 kb on - strand, within SO3528 at 3683.527 kb on + strand, within SO3528 at 3683.534 kb on - strand, within SO3528 at 3683.578 kb on - strand, within SO3528 at 3683.634 kb on + strand, within SO3528 at 3683.653 kb on + strand at 3683.666 kb on + strand at 3683.823 kb on - strand at 3684.956 kb on - strand, within fkbP-2 at 3685.083 kb on - strand, within fkbP-2 at 3685.083 kb on - strand, within fkbP-2 at 3685.117 kb on - strand, within fkbP-2 at 3685.127 kb on - strand, within fkbP-2 at 3685.208 kb on - strand, within fkbP-2
Per-strain Table
Position Strand Gene LocusTag Fraction propionate (C) remove 3,682,928 + SO3527 0.82 -0.6 3,682,956 - SO3527 0.85 +1.8 3,683,037 + -0.1 3,683,239 - SO3528 0.20 +0.8 3,683,354 - SO3528 0.40 -0.8 3,683,392 - SO3528 0.47 +0.4 3,683,412 - SO3528 0.50 +0.1 3,683,527 + SO3528 0.71 -1.4 3,683,534 - SO3528 0.72 +1.2 3,683,578 - SO3528 0.80 -0.5 3,683,634 + SO3528 0.90 -1.2 3,683,653 + -0.9 3,683,666 + -0.6 3,683,823 - +0.7 3,684,956 - fkbP-2 SO3530 0.21 -1.4 3,685,083 - fkbP-2 SO3530 0.51 -2.6 3,685,083 - fkbP-2 SO3530 0.51 -2.5 3,685,117 - fkbP-2 SO3530 0.60 -1.4 3,685,127 - fkbP-2 SO3530 0.62 +0.5 3,685,208 - fkbP-2 SO3530 0.81 -2.0
Or see this region's nucleotide sequence