Experiment: Xylitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1926 and Dshi_1927 are separated by 77 nucleotides Dshi_1927 and Dshi_1928 are separated by 57 nucleotides Dshi_1928 and xerC are separated by 3 nucleotides xerC and Dshi_1930 are separated by 19 nucleotides
Dshi_1926: Dshi_1926 - primosomal protein N' (RefSeq), at 2,012,570 to 2,014,753
_1926
Dshi_1927: Dshi_1927 - putative transaldolase (RefSeq), at 2,014,831 to 2,015,484
_1927
Dshi_1928: Dshi_1928 - protein of unknown function DUF484 (RefSeq), at 2,015,542 to 2,016,249
_1928
Dshi_5002: xerC - hypothetical protein (NCBI), at 2,016,253 to 2,017,179
xerC
Dshi_1930: Dshi_1930 - protein of unknown function DUF6 transmembrane (RefSeq), at 2,017,199 to 2,018,038
_1930
Position (kb)
2015
2016
2017 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2014.740 kb on + strand at 2014.773 kb on + strand at 2014.773 kb on + strand at 2014.850 kb on + strand at 2014.985 kb on - strand, within Dshi_1927 at 2015.080 kb on - strand, within Dshi_1927 at 2015.088 kb on + strand, within Dshi_1927 at 2015.257 kb on - strand, within Dshi_1927 at 2015.308 kb on - strand, within Dshi_1927 at 2015.538 kb on + strand at 2015.546 kb on - strand at 2015.599 kb on + strand at 2015.695 kb on - strand, within Dshi_1928 at 2016.345 kb on + strand at 2017.121 kb on - strand at 2017.121 kb on - strand at 2017.160 kb on - strand at 2017.173 kb on + strand at 2017.181 kb on - strand at 2017.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Xylitol (C) remove 2,014,740 + +0.3 2,014,773 + -5.2 2,014,773 + -3.3 2,014,850 + -3.9 2,014,985 - Dshi_1927 0.24 -2.9 2,015,080 - Dshi_1927 0.38 -2.0 2,015,088 + Dshi_1927 0.39 -2.5 2,015,257 - Dshi_1927 0.65 -3.7 2,015,308 - Dshi_1927 0.73 -2.0 2,015,538 + -0.2 2,015,546 - +0.4 2,015,599 + -0.4 2,015,695 - Dshi_1928 0.22 -0.3 2,016,345 + -1.3 2,017,121 - +0.0 2,017,121 - -0.6 2,017,160 - +1.1 2,017,173 + +0.2 2,017,181 - -1.2 2,017,221 - +0.4
Or see this region's nucleotide sequence